A5017795133- Acute Myeloid Leukemia Research
- DNA Repair Mechanisms
- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- Cancer therapeutics and mechanisms
- Pregnancy and preeclampsia studies
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Blood Coagulation and Thrombosis Mechanisms
- Scientific Computing and Data Management
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Climate variability and models
- Chromosomal and Genetic Variations
- 3D Shape Modeling and Analysis
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- Species Distribution and Climate Change
- Data Analysis with R
- Genomics and Chromatin Dynamics
- Cell Adhesion Molecules Research
- Medical History and Innovations
- Nuclear Structure and Function
- Blood disorders and treatments
- Lysosomal Storage Disorders Research
Yale University
2005-2025
Queen's University
2025
Vermont Fish & Wildlife Department
2025
University of Vermont
2025
Duke University
2023
New York University
2005
University of Pennsylvania
1999
Emory University
1996-1997
Medical University of South Carolina
1992-1996
The UAF1-USP1 complex deubiquitinates FANCD2 during execution of the Fanconi anemia DNA damage response pathway. As such, UAF1 depletion results in persistent ubiquitination and hypersensitivity. UAF1-deficient cells are also impaired for repair by homologous recombination. Herein, we show that binds forms a dimeric with RAD51AP1, an accessory factor RAD51 recombinase, trimeric through RAD51AP1. Two small ubiquitin-like modifier (SUMO)-like domains SUMO-interacting motif RAD51AP1 mediate...
Abstract Fanconi anemia (FA) is a multigenic disease of bone marrow failure and cancer susceptibility stemming from to remove DNA crosslinks other chromosomal lesions. Within the FA damage response pathway, DNA-dependent monoubiquitinaton FANCD2 licenses downstream events, while timely deubiquitination serves extinguish response. Here, we show with reconstituted biochemical systems, which developed, that efficient by USP1-UAF1 complex dependent on binding UAF1. Surprisingly, find activity...
Abstract Wildlife face a myriad of challenges due to changing climate and land use regimes that necessitate efficient monitoring methods on large geospatial scales. This is increasingly accomplished with remotely deployed trail cameras automated recording units. However, remote wildlife comes data management challenges, such as storing, organizing analysing volumes digital data. Data tools can assist biologists in managing projects reduce the barriers between collection dissemination...
Nucleic acid–mediated signaling triggers an immune response that is believed to be central the pathophysiology of autoimmunity in systemic lupus erythematosus (SLE). Here, we found a cell-penetrating, SLE-associated antiguanosine autoantibody may present therapeutic opportunities for cancer treatment. The entered cells through nucleoside salvage-linked pathway membrane transit avoids endosomes and lysosomes bound endogenous RNA live cells. In orthotopic models glioblastoma, antibody...
Several RNA interference (RNAi)-based functional genomic projects have been performed in Caenorhabditis elegans to identify genes required during embryogenesis. These studies demonstrated that the ovary is enriched for transcripts essential first cell divisions. However, comparing RNAi results suggests many involved embryogenesis yet be identified, especially those eliciting partially penetrant phenotypes. To discover additional C. embryonic development, we tested by 1123 ORFeome clones...
USP1-associated factor 1 (UAF1) is an integral component of the RAD51-associated protein (RAD51AP1)-UAF1-ubiquitin-specific peptidase (USP1) trimeric deubiquitinase complex. This complex acts on DNA-bound, monoubiquitinated Fanconi anemia complementation group D2 (FANCD2) in pathway DNA damage response. Moreover, RAD51AP1 and UAF1 cooperate to enhance homologous pairing mediated by recombinase RAD51 repair via recombination (HR) pathway. However, whereas DNA-binding activity has been shown...
Recently, high dose Ara-C (HIDAC) has been shown to induce leukemic cell death in vitro by the alternative process of programmed (PCD) or apoptosis which correlates with inhibition their clonogenic survival. Since co-treatment hemopoietic growth facts (HGFs) GM-CSF and IL-3 have demonstrated enhance metabolism cytotoxic effects HIDAC against progenitor cells, we examined effect HGFs pIXY 321 (a GM-CSF/IL3 fusion protein) G-CSF on induced PCD related gene expressions as well mediated colony...
Some antinuclear antibodies (ANAs) bind extracellular nucleic acids released into tumor environments and are pulled the nuclei of live cancer cells through nucleoside salvage pathways, independent tumor-specific surface antigens. Here we show that ANA nuclear penetration induces flux by lysosomal protease cathepsin B leverage this mechanism to design an antibody-drug conjugate (ANADC) with B-labile drug linker. The ANADC targets acid exhaust from necrotic tumors crosses membrane barriers as...
We propose a new Tipping Point Generative Adversarial Network (TIP-GAN) for better characterizing potential climate tipping points in Earth system models. describe an adversarial game to explore the parameter space of these models, detect upcoming points, and discover drivers points. In this setup, set generators learn construct model configurations that will invoke point. The discriminator learns identify which are generating each configuration whether given lead is trained using oracle (a...