A5090465046- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Reproductive Biology
- Adenosine and Purinergic Signaling
- Plant nutrient uptake and metabolism
- S100 Proteins and Annexins
- Calcium signaling and nucleotide metabolism
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Seed Germination and Physiology
- Fern and Epiphyte Biology
- Plant and Biological Electrophysiology Studies
- Magnetic and Electromagnetic Effects
- Spaceflight effects on biology
- Phytase and its Applications
- Neonatal Respiratory Health Research
- Legume Nitrogen Fixing Symbiosis
- Algal biology and biofuel production
- Nuclear Structure and Function
- Carbon and Quantum Dots Applications
- Polysaccharides and Plant Cell Walls
- Plant and animal studies
- Transgenic Plants and Applications
- Antimicrobial Peptides and Activities
The University of Texas at Austin
2014-2023
University of Minnesota
2012
Hebei Normal University
2011-2012
Wake Forest University
2012
TU Dresden
2007-2011
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2009
Missouri University of Science and Technology
2007
Purdue University West Lafayette
2007
RIKEN
1996
Bial (Portugal)
1984
Abstract Extracellular ATP can serve as a signaling agent in animal cells, and, suggested by recent reports, may also do so plant cells. In cells it induces the production of reactive oxygen species through mediation NADPH oxidase. Similarly, here we report that leaves Arabidopsis (Arabidopsis thaliana), applied ATP, but not AMP or phosphate, accumulation superoxide (O2 −) biphasic, dose-dependent manner, with threshold at 500 nm ATP. This effect did require hydrolysis for was mimicked...
Recently, selective and systemically active antagonists for the metabotropic glutamate 5 receptor (mGlu(5)) were discovered, most potent derivative was found to be MPEP (2-methyl-6-(phenylethynyl)pyridine). Given high expression of mGlu(5) receptors in limbic forebrain regions, it decided evaluate anxiolytic potential MPEP. After an acute oral administration, attenuated anxiety-dependent variable a variety well established anxiety test paradigms. In rats, (10, 30, 100 mg/kg) increased...
Abstract Annexins act as targets of calcium signals in eukaryotic cells, and recent results suggest that they play an important role plant stress responses. We found Arabidopsis (Arabidopsis thaliana), AnnAt1 (for annexin 1) mRNA levels were up-regulated leaves by most the treatments applied. Plants overexpressing protein more drought tolerant knockout plants sensitive than ecotype Columbia plants. also observed hydrogen peroxide accumulation guard cells was reduced increased both before...
Extracellular ATP is a known receptor agonist in animals and was previously shown to alter plant growth, so we investigated whether derivatives could function outside cells as signaling agents. Signaling responses induced by exogenous nucleotides animal typically include increases free cytoplasmic calcium concentration ([Ca2+]cyt). We have evaluated the ability of exogenously applied adenosine 5′-[γ-thio]triphosphate (ATPγS), 5′-[β-thio]diphosphate (ADPβS), 5′-O-thiomonophosphate [Ca2+]cyt...
Abstract Expression of two Arabidopsis (Arabidopsis thaliana) apyrase (nucleoside triphosphate-diphosphohydrolase) genes with high similarity, APY1 and APY2, was analyzed during seedling development under different light treatments using β-glucuronidase fusion constructs the promoters both genes. As evaluated by staining independently confirmed other methods, highest expression apyrases in rapidly growing tissues and/or that accumulate auxin levels. Red-light treatment etiolated seedlings...
Both scientists and the public would benefit from improved communication of basic scientific research integrating into education outreach, but opportunities to support these efforts are limited. We have developed two low-cost programs—"Present Your PhD Thesis a 12-Year-Old" "Shadow Scientist"—that combine training in science with outreach area middle schools. assessed outcomes programs found 2-fold benefit: improve their skills by explaining general audience, students' enthusiasm for...
Xenobiotic resistance in animals, plants, yeast, and bacteria is known to involve ATP binding cassette transporters that efflux invading toxins. We present data from yeast a higher plant indicating xenobiotic also involves extracellular degradation. Transgenic upregulation of ecto-ATPase alone confers organisms have had no previous exposure Similarly, cells are deficient ATPase activity more sensitive xenobiotics. On the basis these other supporting data, we hypothesize hydrolysis by...
Abstract Although in most plant species no more than two annexin genes have been reported to date, seven homologs identified Arabidopsis, Annexin Arabidopsis 1–7 (AnnAt1–AnnAt7). This establishes that annexins can be a diverse, multigene protein family single species. Here we compare and analyze these gene sequences present the situ RNA localization patterns of genes, AnnAt1 andAnnAt2, during different stages development. Sequence analysis AnnAt1–AnnAt7 reveals they contain characteristic...
Abstract In Arabidopsis, we previously identified two highly similar apyrases, AtAPY1 and AtAPY2. Here, T-DNA knockout (KO) mutations of each gene were isolated in a reverse genetic approach. The single KO mutants lacked discernible phenotype. double mutants, however, exhibited complete inhibition pollen germination, this correlated with positive β-glucuronidase staining the apyrase promoter:β-glucuronidase fusion transgenic lines. vast majority grains these identical to wild type size,...
Abstract Raising the level of extracellular ATP to mmconcentrations similar those found inside cells can block gravitropism Arabidopsis roots. When plants are grown in Murashige and Skoog medium supplied with 1 mm ATP, their roots grow horizontally instead growing straight down. Medium 2 induces root curling, 3 stimulates lateral growth. transferred containing exogenous gravity response is reduced or some cases completely blocked by ATP. Equivalent concentrations ADP inorganic phosphate have...
A protein identifiable as calmodulin has been isolated from oat (Avena sativa, var Garry) tissues. This is relatively heat stable, binds to hydrophobic gels, and phenothiazines in a calcium-dependent fashion, antibody rat testes calmodulin. Based on its migration sodium dodecyl sulfate-polyacrylamide ultraviolet absorption spectrum, amino acid composition, essentially identical other higher plants. Radioimmunoassays indicate that associated with protoplasts, mitochondria, etioplasts, nuclei...
Plant and animal cells release or secrete ATP by various mechanisms, this activity allows extracellular to serve as a signalling molecule. Recent reports suggest that induces plant responses ranging from increased cytosolic calcium changes in auxin transport, xenobiotic resistance, pollen germination, growth. Although has been identified secondary messenger for the signal, other parts of signal transduction chain remain unknown. Increasing concentration ATPgammaS, poorly-hydrolysable...
Abstract This study investigates the role of extracellular nucleotides and apyrase enzymes in regulating stomatal aperture. Prior data indicate that expression two apyrases Arabidopsis (Arabidopsis thaliana), APY1 APY2, is strongly correlated with cell growth secretory activity. Both are expressed guard protoplasts, as determined by reverse transcription-polymerase chain reaction immunoblot analyses. Promoter activity assays for APY2 show both correlates conditions favor opening....
Abstract Plant cells release ATP into their extracellular matrix as they grow, and (eATP) can modulate the rate of cell growth in diverse tissues. Two closely related apyrases (APYs) Arabidopsis (Arabidopsis thaliana), APY1 APY2, function, part, to control concentration eATP. The expression APY1/APY2 be inhibited by RNA interference, this suppression leads an increase eATP medium severely reduces growth. To clarify how APY2 is linked inhibition, gene changes that occur seedlings when apyrase...
Abstract ATP, which is present in the extracellular matrix of multicellular organisms and fluid unicellular organisms, has been shown to function as a signaling molecule animals. The concentration ATP (xATP) known be functionally modulated part by ectoapyrases, membrane-associated proteins that cleave γ- β-phosphates on xATP. We data showing previously unreported (to our knowledge) linkage between apyrase phosphate transport. An from pea (Pisum sativum) complements yeast (Saccharomyces...
The structure, function, and mechanism of action plant calmodulins have been the subject intense study for scientists during past several years. While precedents in animal biochemistry physiology suggested logical starting points studies how calmodulin is involved higher algal cell recent biochemical demonstrated unique structural characteristics this highly conserved protein. Enzym‐ological analyses novel functional properties provided limited insight into molecular mechanisms action. This...