A5100378838- Electrocatalysts for Energy Conversion
- Advanced battery technologies research
- Plant nutrient uptake and metabolism
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Animal Virus Infections Studies
- Electrochemical Analysis and Applications
- Animal Disease Management and Epidemiology
- Plant Molecular Biology Research
- Virus-based gene therapy research
- Polysaccharides and Plant Cell Walls
- Insect symbiosis and bacterial influences
- Soybean genetics and cultivation
- Plant Micronutrient Interactions and Effects
- Cocoa and Sweet Potato Agronomy
- Plant-Microbe Interactions and Immunity
- Iron oxide chemistry and applications
- Chalcogenide Semiconductor Thin Films
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Antimicrobial Peptides and Activities
- Viral gastroenteritis research and epidemiology
- Plant Stress Responses and Tolerance
- Antibiotic Resistance in Bacteria
- Architecture and Computational Design
Jilin University
2021-2024
Sanya University
2024
Hainan University
2024
Shihezi University
2020-2024
Nanjing Agricultural University
2020-2023
Sun Yat-sen University
2023
Dalian Polytechnic University
2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Zhejiang University
2022
Zhejiang Ocean University
2022
Xylella fastidiosa is a xylem-limited nonflagellated bacterium that causes economically important diseases of plants by developing biofilms block xylem sap flow. How the translocated downward in host plant's vascular system against direction transpiration stream has long been puzzling phenomenon. Using microfabricated chambers designed to mimic some features vessels, we discovered X. migrates via type IV-pilus-mediated twitching motility at speeds up 5 mum min(-1) rapidly flowing medium...
Xylella fastidiosa, an important phytopathogenic bacterium, causes serious plant diseases including Pierce's disease of grapevine. It is reported here that type I and IV pili X. fastidiosa play different roles in twitching motility, biofilm formation cell–cell aggregation. Type are particularly for aggregation, whereas essential also function formation. Thirty twitching-defective mutants were generated with EZ : TN transposome system, several type-IV-pilus-associated genes identified, fimT,...
Xylella fastidiosa, a bacterium responsible for Pierce's disease in grapevines, possesses both type I and IV pili at the same cell pole. Type facilitate twitching motility, are involved biofilm development. The adhesiveness of bacteria roles two types attachment to glass substratum were evaluated using microfluidic flow chamber conjunction with pilus-defective mutants. average adhesion force necessary detach wild-type X. fastidiosa cells was 147 +/- 11 pN. Mutant possessing only required 204...
In plants, male sterility is an important agronomic trait, especially in hybrid crop production. Many factors are known to affect sterility, but it remains unclear whether Suc transporters (SUTs) participate directly this process. Here, we identified and functionally characterized the cucumber (Cucumis sativus) CsSUT1, a typical plasma membrane-localized energy-dependent high-affinity Suc-H+ symporter. CsSUT1 expressed flowers encodes protein that localized primarily tapetum, pollen,...
Phloem loading, as the first step of transporting photoassimilates from mesophyll cells to sieve element-companion cell complex, creates a driving force for long-distance nutrient transport. Three loading strategies have been proposed: passive symplastic apoplastic and transfer followed by polymer-trapping stachyose raffinose. Although individual species are generally referred using single phloem mechanism, it has suggested that some plants may use more than one, i.e. 'mixed loading'. Here,...
Abstract The devise and fabrication of highly efficient electrocatalysts have momentous practical significance for the development future hydrogen energy systems. However, their potential uses in support construction are not well studied. In this work, a electrocatalyst is developed evolution reaction by anchoring single Pt atoms clusters into functional amorphous pentlandite Fe 5 Ni 4 S 8 (or FNS) to yield Pt–FNS composites. Amorphous FNS still provides great conductivity composites,...
In the fleshy fruit of cucumbers (Cucumis sativus L.), phloem flow is unloaded via an apoplasmic pathway, which requires protein carriers to export sugars derived from stachyose and raffinose into apoplasm. However, transporter(s) involved in this process remain unidentified. Here, we report that a hexose transporter, CsSWEET7a (Sugar Will Eventually be Exported Transporter 7a), was highly expressed cucumber sink tissues localized plasma membrane companion cells phloem. Its expression level...
Sugars are necessary for plant growth and fruit development. Cucumber (Cucumis sativus L.) transports sugars, mainly raffinose family oligosaccharides (RFOs), in the vascular bundle. As dominant sugars cucumber fruit, glucose fructose derived from sucrose, which is product of RFO hydrolysis by α-galactosidase (α-Gal). Here, we characterized alkaline 2 (CsAGA2) gene found that CsAGA2 has undergone human selection during domestication. Further experiments showed expression increases gradually...
The emerging and global spread of a novel plasmid-mediated colistin resistance gene, mcr-1 , threatens human health. Expression the MCR-1 protein affects bacterial fitness this cost correlates with lipid A perturbation. However, exact molecular mechanism remains unclear. Here, we identified M6 variant carrying two-point mutations that conferred co-resistance to β-lactam antibiotics. Compared wild-type (WT) MCR-1, caused severe disturbance in A, resulting up-regulation L, D-transpeptidases...
Xylella fastidiosa is an important phytopathogenic bacterium that causes many serious plant diseases, including Pierce's disease of grapevines. Disease manifestation by X. associated with the expression several factors, type IV pili are required for twitching motility. We provide evidence operon, named Pil-Chp, genes homologous to those found in chemotaxis systems, regulates Transposon insertion into pilL gene operon resulted loss motility (pilL cheA encoding kinases). The mutant maintained...
The functions of SWEET (Sugar Will Eventually be Exported Transporter) proteins have been studied in a number crops, but little is known about their roles cucumber (Cucumis sativus L.), model plant for studying stachyose metabolism and phloem function. Here, we identified 17 genes (CsSWEETs), located on chromosomes 1-6, classified them into four clades. Two from each clade were selected spatiotemporal expression, subcellular localization, substrate specificity analyses. Clade I II all hexose...
A mutation in the Xylella fastidiosa tonB1 gene resulted loss of twitching motility and significantly less biofilm formation as compared with a wild type. The altered phenotypes were restored by complementation functional copy gene. affected virulence measured Pierce's disease symptoms on grapevines. role TonB1 appears to be independent characteristic iron-uptake function this protein. This is first report demonstrating for tonB homolog X. fastidiosa.
Abstract Pythium and Phytopythium spp. cause seed decay, damping off, root rot in soybean, wheat, many other crops. However, their diversity importance as pathogens, particularly different crop rotation systems, are largely unknown. A survey was conducted the Huang‐Huai region, one of main areas soybean–wheat farming China. In 2016–2018, we collected 300 soybean seedlings 150 field soil samples from several representative locations, identified 26 6 212 isolates, based on internal transcribed...
Heterogenous catalysis is important for future clean and sustainable energy systems. However, an urgent need to promote the development of efficient stable hydrogen evolution catalysts still exists. In this study, ruthenium nanoparticles (Ru NPs) are in situ grown on Fe5 Ni4 S8 support (Ru/FNS) by replacement growth strategy. An Ru/FNS electrocatalyst with enhanced interfacial effect then developed successfully applied pH-universal reaction (HER). The Fe vacancies formed FNS during...
Abstract Background Bovine viral diarrhea (BVD) is an acute febrile infectious disease caused by the bovine virus (BVDV), which has brought huge economic losses to world's cattle industry. At present, commercial inactivated BVDV vaccines may cause some adverse reactions during use. This study aims develop a safer and more efficient vaccine. Methods Here, we described generation preclinical efficacy of hydrogen peroxide (H 2 O ) type 1 vaccine in mice, administered it separately with...