A5034248641- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Tuberculosis Research and Epidemiology
- Evolution and Genetic Dynamics
- Mycobacterium research and diagnosis
- Cancer therapeutics and mechanisms
- Lipid Membrane Structure and Behavior
- Immune Response and Inflammation
- Pharmaceutical and Antibiotic Environmental Impacts
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Antibiotic Use and Resistance
- Vibrio bacteria research studies
- Microbial Metabolic Engineering and Bioproduction
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
Sun Yat-sen University
2020-2024
Fifth Affiliated Hospital of Sun Yat-sen University
2022-2024
The First Affiliated Hospital, Sun Yat-sen University
2022-2024
Sun Yat-sen University Cancer Center
2024
South China Agricultural University
2021
The global dissemination of the mobilized colistin resistance gene, mcr-1, threatens human health. Recent studies by our group and others have shown that withdrawal as a feed additive dramatically reduced prevalence mcr-1. Although it is accepted rapid reduction in mcr-1 may resulted, to some extent, from toxic effects MCR-1, detailed mechanism remains unclear. Here, we found MCR-1 damaged outer membrane (OM) permeability Escherichia coli Klebsiella pneumonia this event was associated with...
The emerging and global spread of a novel plasmid-mediated colistin resistance gene, mcr-1 , threatens human health. Expression the MCR-1 protein affects bacterial fitness this cost correlates with lipid A perturbation. However, exact molecular mechanism remains unclear. Here, we identified M6 variant carrying two-point mutations that conferred co-resistance to β-lactam antibiotics. Compared wild-type (WT) MCR-1, caused severe disturbance in A, resulting up-regulation L, D-transpeptidases...
There is growing evidence that GreA aids adaptation to stressful environments in various bacteria. However, the functions of among mycobacteria remain obscure. Here, we report on cellular consequences following deletion greA gene Mycobacterium spp. The mutant strain (ΔgreA) was generated smegmatis, tuberculosis H37Ra, and H37Rv. Deletion results growth retardation poor survival response adverse stress, besides rendering M. more susceptible vancomycin rifampicin. By using RNA-seq, observe...
A sharp increase in multidrug-resistant tuberculosis (MDR-TB) threatens human health. Spontaneous mutation essential gene confers an ability of Mycobacterium resistance to anti-TB drugs. However, conventional laboratory strategies for identification and prediction the mutations this slowly growing species remain challenging. Here, by combining XCas9 nickase error-prone DNA polymerase from M. tuberculosis, we constructed a CRISPR-guided system, CAMPER, effective site-directed mutagenesis...
Type I and type II CRISPR-Cas systems are employed to evade host immunity by targeting interference of bacteria's own genes. Although Mycobacterium tuberculosis (M. tuberculosis), the causative agent tuberculosis, possesses integrated III-A system, its role in mycobacteria remains obscure. Here, we observed that seven cas genes (csm2∼5, cas10, cas6) were upregulated bovis BCG under oxidative stress treatment, indicating system stress. To explore functional TCC (Type including cas6, csm2-6)...
The Pseudomonas putida F1 genome contains five genes annotated as encoding 3-ketoacyl-acyl carrier protein (ACP) synthases. Four are FabF (3-ketoacyl-ACP synthase II) proteins, and the fifth is a FabB I) protein. Expression of one FabF2, cryptic in native host becomes physiologically important only when repressor controlling fabF2 transcription inactivated. When derepressed, FabF2 can functionally replace FabB, expressed from foreign promoter, had weak activity. Complementation Escherichia...
The antibiotic resistance crisis continues to threaten human health. Better predictions of the evolution genes could contribute design more sustainable treatment strategies. However, comprehensive prediction gene via laboratory approaches remains challenging. By combining site-specific integration and high-throughput sequencing, we quantified relative growth under respective selection cefotaxime or ceftazidime in ∼23,000 Escherichia coli MG1655 strains that each carried a unique, single-copy...
The rapid emergence of multidrug-resistant/extensively drug-resistant tuberculosis (TB) is responsible for treatment failure in patients with TB and significantly endangers global public health. Recently, bioenergetics has become a new paradigm anti-TB drug discovery based on the link between bacterial ATP levels efficacy. A better understanding role fluctuations during antibiotic may provide insight into antibiotic-mediated killing mycobacteria. Here, we employed an advanced...
Abstract The emergence and global discovery of the mobilized colistin resistance gene, mcr-1 , limits clinical effectiveness as last-resort antibiotic against multiple-drug resistant bacteria. Although MCR-1 exhibits low levels in E. coli it is currently unknown whether may potentially evolve a high level future. Here, we generated mutant library that included 27,965 variants, covering 94.44% single-nucleotide mutations. We quantified relative growth these variant strains under exposure....
Rifampicin is the most powerful first-line antibiotic for tuberculosis, which caused by Mycobacterium tuberculosis. Although accumulating evidence from sequencing data of clinical M. tuberculosis isolates suggested that mutations in rifampicin-resistance-determining region (RRDR) are strongly associated with rifampicin resistance, comprehensive characterization RRDR polymorphisms confer this resistance remains challenging. By incorporating I-SceI sites I-SceI-based integrant removal and...
Colistin (CT) is the last-resort of antibiotic against multidrug-resistance (MDR) Acinetobacter baumannii ( A. ) infection. However, colistin resistance increasingly reported in isolates partially due to global emergence and dissemination plasmid-borne mobile mcr gene a threat human health. Thus, available treatment strategies urgently required fight colistin-resistant . Here, we showed that confers damaged outer membrane (OM) permeability , which could compromise viability Consistently,...
Abstract The emerging and global spread of a novel plasmid-mediated colistin resistance gene, mcr-1 , threatens human health. It is accepted that MCR-1 affects bacterial fitness, this fitness cost correlates with membrane lipid A perturbation. However, the detailed molecular mechanism remains unclear. Here, we screened out variant, named M6, two-point mutation rendered low level co-resistance to β-lactam antibiotics. Compared wild-type (WT) MCR-1, variant caused severe perturbation resulting...