A5101579170- Meat and Animal Product Quality
- Bacterial Genetics and Biotechnology
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Legume Nitrogen Fixing Symbiosis
- Bacterial biofilms and quorum sensing
- Entomopathogenic Microorganisms in Pest Control
- Food Quality and Safety Studies
- Insect Resistance and Genetics
- Advanced Photocatalysis Techniques
- Postharvest Quality and Shelf Life Management
- Gas Sensing Nanomaterials and Sensors
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- Animal Nutrition and Physiology
- Insect-Plant Interactions and Control
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Plant Physiology and Cultivation Studies
- Enzyme Structure and Function
- Bee Products Chemical Analysis
- Metabolomics and Mass Spectrometry Studies
- Radiation Effects and Dosimetry
- Insect and Pesticide Research
South China Agricultural University
2015-2024
Changzhou University
2023-2024
Beijing Academy of Agricultural and Forestry Sciences
2011-2023
Inner Mongolia University of Technology
2023
Animal Science Research Institute
2022
Anyang Institute of Technology
2022
Shanghai Academy of Agricultural Sciences
2008-2021
Key Laboratory of Guangdong Province
2021
Shanghai Jiao Tong University
2019-2021
Chinese Academy of Agricultural Sciences
2011-2021
Triclosan, a very widely used biocide, specifically inhibits fatty acid synthesis by inhibition of enoyl-acyl carrier protein (ACP) reductase. Escherichia coli FabI is the prototypical triclosan-sensitive enoyl-ACP reductase, and E. extremely sensitive to biocide. However, other bacteria are resistant triclosan, because they encode triclosan-resistant reductase isozymes. In contrast, triclosan resistance Pseudomonas aeruginosa PAO1 has been attributed active efflux compound (R. Chuanchuen,...
Mikania micrantha is one of the top 100 worst invasive species that can cause serious damage to natural ecosystems and substantial economic losses. Here, we present its 1.79 Gb chromosome-scale reference genome. Half genome composed long terminal repeat retrotransposons, 80% which have been derived from a significant expansion in past million years. We identify whole duplication event recent segmental duplications, may be responsible for rapid environmental adaptation. Additionally, show M....
The plasmid-encoded colistin resistance gene mcr-1 challenges the use of polymyxins and poses a threat to public health. Although IncI2-type plasmids are most common vector for spreading gene, mechanisms by which these adapt host bacteria maintain genes remain unclear. Herein, we investigated regulatory mechanism controlling fitness cost an IncI2 plasmid carrying mcr-1. A putative ProQ/FinO family protein encoded plasmid, designated as PcnR (plasmid copy number repressor), balances...
Abstract Members of the diffusible signal factor (DSF) family are a novel class quorum sensing (QS) signals in diverse Gram-negative bacteria. Although previous studies have identified RpfF as key enzyme for biosynthesis DSF signals, many questions their remain to be addressed. In this study with phytopathogen Xanthomonas campestris pv. ( Xcc) , we show that Xcc produces four DSF-family (DSF, BDSF, CDSF and IDSF) during cell culture IDSF is new functional characterized cis...
Signal molecules of the diffusible signal factor (DSF) family have been shown recently to be involved in regulation pathogenesis and biofilm formation diverse Gram-negative bacteria. DSF signals are reported active not only on their cognate bacteria, but also unrelated bacteria pathogenic yeast, Candida albicans. DSFs monounsaturated fatty acids medium chain length containing an unusual cis-2 double bond. Although genetic analyses had identified genes synthesis, pathway synthesis was...
Summary In X anthomonas campestris pv . ( Xcc ), the proteins encoded by rpf (regulator of pathogenicity factor) gene cluster produce and sense a fatty acid signal molecule called diffusible signalling factor DSF , 2( Z )‐11‐methyldodecenoic acid). RpfB was reported to be involved in processing predicted encode an acyl‐ CoA ligase. We report that activates wide range acids their esters vitro Moreover, can functionally replace paradigm bacterial ligase, E scherichia coli FadD ß‐oxidation...
Enoyl-acyl carrier protein (enoyl-ACP) reductase catalyzes the last step of elongation cycle in synthesis bacterial fatty acids. The Enterococcus faecalis genome contains two genes annotated as enoyl-ACP reductases, a FabI-type and FabK-type reductase. We report that expression either proteins restores growth an Escherichia coli fabI temperature-sensitive mutant strain under nonpermissive conditions. In vitro assays demonstrated both support acid are active with substrates all chain lengths....
With the characteristic of flexible and precise, unmanned aerial vehicles (UAVs) for low volume applications are increasing substantially quickly around globe. However, little attention has been paid to study wheat herbicides with UAV, especially research on spray droplet size herbicide sprayed by UAVs. The objectives this were compare deposition from a typical commercial UAV under four different volumes 7.5 L/hm2, 15.0 22.5 30.0 L/hm2 three median diameter (VMD) 150 μm, 200 300 μm during...
Abstract BACKGROUND Effective utilization of plant protection UAVs in peanut cultivation management necessitates a comprehensive grasp how application volume rates and pesticides influence leaf spot rust control. This study aimed to compare the effects on droplet deposition, disease, retention rate yield. A T20 unmanned aerial vehicle (UAV) sprayer was used apply four various pesticide doses. In comparison, knapsack spray with an 450 L ha −1 . RESULTS The results showed significant...
Pseudomonas aeruginosa is extremely resistant to triclosan. Previous studies have shown that P. encodes a triclosan-resistant enoyl-acyl-carrier protein reductase (ENR), FabV, and deletion of fabV causes be sensitive In this report, we complemented strain with several ENR encoding genes, including Vibrio cholerae fabV, Bacillus subtilis fabL Enterococcus faecalis fabK. All strains restored triclosan resistance the level wild-type strain, which confirmed allows Moreover, exhibits pleiotropic...
Mikania micrantha is a noxious invasive plant causing enormous economic losses and ecological damage. Soil microbiome plays an important role in the invasion process of M. micrantha, while little known about its rhizosphere composition function. In this study, we identified distinct microbial communities by comparing them with those two coexisting native plants (Polygonum chinense Paederia scandens) bulk soils, using metagenomics data from field sampling pot experiment. As result, enrichment...
Thigh meat from Cobb 500 broiler (Cobb) and Beijing-You chicken (BYC) at marketable age were compared to explore the impact of growth rate on quality. BYC had a higher water capacity, as well lower shear forces IMF content than Cobb, indicating that has significant influence Additionally, 144 upregulated 44 downregulated differential metabolites identified using gas chromatography mass spectroscopy (GC-MS) liquid spectrometer (LC-MS). Moreover, we found difference between dominant expression...
n bacteria, the metabolism of branched-chain amino acids (BCAAs) is tightly associated with fatty acid (BCFA) synthetic pathways. Although previous studies have reported on BCFA biosynthesis, more detailed associations between BCAA and biosynthesis remain to be addressed. In this study, we deleted ilvC gene, which encodes ketol-acid reductoisomerase in pathway, from Xanthomonas campestris pv. (Xcc) genome. We characterized gene functions production diffusible signal factor (DSF) family...
The increasing prevalence of multidrug-resistant Helicobacter pylori strains has created an urgent need for alternative therapeutic regimens that complement the current antibiotic treatment strategies H. eradication; however, this is greatly hampered due to a lack “druggable” targets. Although CFAs are present in cytoplasmic membranes at high levels, their physiological role not been established. In report, deletion CFA synthase CfaS was shown attenuate acid and drug resistance, immune...
The complement system acts as an integral part of the innate immune response, which primarily to remove pathogens and injured cells. Emerging evidence has shown activation regulatory function complements in tumor microenvironment (TME). We revealed expression levels various human cancers their role prognosis infiltration.The differential was explored via Tumor Immune Estimation Resource (TIMER) site Oncomine database. To investigate whether these differentially expressed have correlation...
The condensation of acetyl-CoA with malonyl-acyl carrier protein (ACP) by β-ketoacyl-ACP synthase III (KAS III, FabH) and decarboxylation malonyl-ACP decarboxylase are the two pathways that initiate bacterial fatty acid synthesis (FAS) in Escherichia coli. In addition to these routes, we report Pseudomonas putida F1 I (FabB), playing a key role elongation, also initiates FAS vivo. We although P. fabH genes (PpfabH1 PpfabH2) both encode functional KAS enzymes, neither is essential for growth....