The hypofunction of osteoblasts induced by glucocorticoids (GCs) has been identified as a major contributing factor for GC-induced osteoporosis (GIO). However, the biological mechanism underlying effect GC in are not fully elucidated. Recent studies implicated an important role phosphoinositide 3-kinase (PI3K)/protein kinase B(Akt) signaling pathway regulation bone growth. We propose that PI3K/Akt may be process osteogenic inhibition osteoblasts. In this study, primary were used vitro and...

The epithelial-mesenchymal transition (EMT) induced by EGF promotes cervical cancer progression; however, the mechanisms underlying EGF-induced EMT remain unclear. In this study, we reported that miR155 overexpression suppressed EMT, decreased migration/invasion capacities, inhibited cell proliferation and increased chemo-sensitivity to DDP in human Caski cells. Further, of TP53 expression but reduced SMAD2, CCND1 levels. These data suggest negatively regulates EMT. We conclude does not act...

Teleost fish anterior kidney (AK) is an important hematopoietic organ with multifarious immune cells, which have functions comparable to mammalian bone marrow. Myeloid and lymphoid cells locate in the AK, but lack of useful specific gene markers antibody-based reagents for cell subsets makes identification different types difficult. Single-cell transcriptome sequencing enables single-cell capture individual library construction, making study on heterogeneity teleost AK possible. In this...

Even though nuclear factor-erythroid 2-related factor 2 (Nrf2) signaling has been associated with the pathogenesis of multiple heart conditions, data on roles Nrf2 within atrial fibrillation (AF) still remain scant. The present investigation had aim analyzing Nrf2-overexpressing role/s upon bone mesenchymal stem cell- (BMSC-) derived exosomes in rats AF.Exosomes were collected from control or lentivirus-transduced BMSCs and then injected into AF through tail vein. duration was observed using...

Background Stroke is the second leading cause of death and third disability worldwide, with ischemic stroke (IS) being most prevalent. A substantial number irreversible brain cell occur in short term, to impairment or IS. Limiting loss cells primary therapy target a significant clinical issue for IS therapy. Our study aims establish gender specificity pattern from immune infiltration four kinds cell-death perspectives improve diagnosis Methods Combining standardizing two datasets (GSE16561...

Abstract BACKGROUND Effective utilization of plant protection UAVs in peanut cultivation management necessitates a comprehensive grasp how application volume rates and pesticides influence leaf spot rust control. This study aimed to compare the effects on droplet deposition, disease, retention rate yield. A T20 unmanned aerial vehicle (UAV) sprayer was used apply four various pesticide doses. In comparison, knapsack spray with an 450 L ha −1 . RESULTS The results showed significant...

Abstract Bladder cancer is one of the most common genitourinary tract cancers. Non-muscle-invasive bladder (NMIBC) represents type. Intravesical immunotherapy with Bacillus Calmette-Guerin has been used to treat patients high-risk NMIBC after surgery. However, 50-70% experience disease recurrence and progression. Therefore, identifying novel therapeutic strategies for needed. Nectin cell adhesion molecule 4 (NECTIN4) an immunoglobulin-like transmembrane protein which involved in formation...

This study aimed to identify circular RNAs differentially expressed in the islets of type 2 diabetes (T2DM) models and clarify their roles control β-cell functions. Circular dysregulated diabetic db/db mice were identified by high-throughput RNA sequencing. Then, expression level selected circ-Tulp4 was confirmed real-time PCR Min6 cells. MTS, EdU, western blot, flow cytometric analysis, luciferase assay performed investigate impact on thousands mouse pancreatic islets. The significantly...

Flavor is an important quality of mature tomato fruits. Compared with heirloom tomatoes, modern commercial cultivars are considerably less flavorful. This study aimed to compare the flavor 71 accessions (8 pink cherry, PC; 11 red RC; 15 large-fruited, PL; and 37 RL) using hedonism scores odor activity values. Taste compounds were detected high-performance liquid chromatography. Volatiles gas chromatography–olfactometry–mass spectrometry. The can be evaluated DTOPSIS analysis method....

Angiogenesis enhances cancer metastasis and progression, however, the roles of transcription regulation in angiogenesis are not fully defined. ZNF322A is an oncogenic zinc-finger factor. Here, we demonstrate a new mechanism Kras mutation-driven transcriptional activation elucidate interplay between its upstream regulators downstream targets promoting neo-angiogenesis. Methods: Luciferase activity, RT-qPCR ChIP-qPCR assays were used to examine cell models. In vitro vivo conducted....

The epidermal growth factor receptor (EGF-R) signaling pathway is thought to have an important role in the development and progression of several carcinomas, as it associated with cell proliferation, differentiation migration. Activation EGF-R regulates epithelial-mesenchymal transition (EMT)-associated invasion migration normal malignant epithelial cells. However, specific mechanisms not yet been fully elucidated. present study utilized wound healing assays, western blotting, flow cytometry...

Abstract Increasing evidence indicates that long noncoding RNAs (lncRNAs) have crucial roles in various biological processes. However, the contribution of lncRNAs to β-cell dysfunction and their diabetes therapeutics remain poorly understood. The aim this study was identify dysregulated diabetic islets explore involved function as potential therapeutic targets. By using RNA sequencing real-time PCR, we identified thousands db/db mice db/m littermate mice. Among differentially expressed...

(TF) stem accounts for about 10% of the whole mushroom, and most them are dumped without utilization. To effectively utilize waste, effects fine grinding superfine technology on processing characteristics physicochemical properties

Anticoagulant therapy can significantly reduce the incidence of stroke and peripheral embolism events in patients with atrial fibrillation (AF). Although warfarin is widely used as an anticoagulant drug, a wrong dose lead to increased risks bleeding or blood clots. The aim this study was assess whether nuclear factor-erythroid-2-related factor 2 (Nrf2) improve efficacy through regulation cytochrome P450 family subfamily C member 9 (CYP2C9) using rat model AF. Results showed that AF reduced...

Background Liver fibrosis was viewed as a reversible process. The activation of hepatic stellate cells (HSCs) is key event in the process liver fibrosis. induction senescence HSCs would accelerate clearance activated HSCs. Previously, we demonstrated that soluble egg antigens (SEA) Schistosoma japonicum promoted via STAT3/P53/P21 pathway. In this paper, our study aimed to explore whether there are other signaling pathways SEA-induced aging and underlying effect SKP2/P27 signal on senescent...

Abstract miR‐125a is a microRNA that frequently diminished in various human malignancies. However, the mechanism by which impaired promotes cancer growth remains undefined. In this study, we investigated role of proliferation and apoptosis multiple myeloma (MM). To do this, used MM tissue samples (from 40 anonymous patients), normal matched control samples, five MM‐derived cell lines. We also established mouse model xenograft to explore effect overexpression on vivo. Quantitative real‐time...

Essential hypertension (EH) is a common and multifactorial disorder that likely to be influenced by multiple genes. The methylenetetrahydrofolate reductase (

Abstract Toxoplasma gondii is an opportunistic intracellular parasite and considered important aetiological factor in the process of abortion, especially as occurs early gestation. Chinese 1 strain T. a dominant genotype prevalent China. Although it known that foetal resorption triggered by RH attributable to immune mechanisms rather than its direct effect uterus, underlying mechanism abortion caused remains unclear. This study was designed investigate excreted–secreted antigens (ESA) on...

Background: Increasing evidences have revealed that solasodine, isolated from Solanum sisymbriifolium fruits, has multiple functions such as anti-oxidant, anti-tumor and antiinfection.However, its role in pancreatic cancer not been well studied.Methods: To explore the of solasodine cancer, human cell lines including SW1990 PANC1 were treated with different concentrations for 48 h, viability was evaluated by MTT assay, invasion migration Transwell assay.The effect on apoptosis cells detected...