A5029374349- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Horticultural and Viticultural Research
- Plant and animal studies
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Viral Infections and Immunology Research
- Plant biochemistry and biosynthesis
- Yeasts and Rust Fungi Studies
- Viral Infections and Vectors
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Enzyme Production and Characterization
- 14-3-3 protein interactions
- COVID-19 Clinical Research Studies
- Spine and Intervertebral Disc Pathology
- Cervical and Thoracic Myelopathy
- Herpesvirus Infections and Treatments
- Steroid Chemistry and Biochemistry
- Food Quality and Safety Studies
- Algal biology and biofuel production
- Atrial Fibrillation Management and Outcomes
- Cardiovascular Function and Risk Factors
- Medical Imaging and Analysis
Yangzhou University
2019-2025
Nanjing Agricultural University
2024
Beijing Anzhen Hospital
2023
Capital Medical University
2023
Key Laboratory of Guangdong Province
2020
South China Normal University
2020
Yunnan Academy of Agricultural Sciences
2014-2018
Defense Information Systems Agency
2017
Chinese Academy of Forestry
2015
Fujian Agriculture and Forestry University
2015
Mikania micrantha is one of the top 100 worst invasive species that can cause serious damage to natural ecosystems and substantial economic losses. Here, we present its 1.79 Gb chromosome-scale reference genome. Half genome composed long terminal repeat retrotransposons, 80% which have been derived from a significant expansion in past million years. We identify whole duplication event recent segmental duplications, may be responsible for rapid environmental adaptation. Additionally, show M....
To date, four species of porcine circoviruses (PCVs), including PCV1-4, have been reported to exist in the clinical cases. Fast and effective differential detection is critical monitor infection co-infection status PCVs for adopting reliable control strategies. However, currently available methods cannot simultaneously differentiate PCV strains. In this study, a quadruplex real-time PCR assay based on TaqMan probes was developed PCV1-4. The new exhibited satisfied specificity, sensitivity,...
Rosa chinensis var. spontanea, an endemic and endangered plant of China, is one the key ancestors modern roses a source for famous traditional Chinese medicines against female diseases, such as irregular menses dysmenorrhea. In this study, complete chloroplast (cp) genome R. spontanea was sequenced, analyzed, compared to congeneric species. The cp typical quadripartite circular molecule 156,590 bp in length, including large single copy (LSC) region 85,910 small (SSC) 18,762 bp, separated by...
Since we first reported porcine reproductive and respiratory syndrome virus 1 (PRRSV1) wild type strains in mainland China 2011, PRRSV1 infection has been detected more than 20 provinces China. During the routine investigation of epidemiology 2022, isolated a novel strain (SD1291) from an adult slaughter pig Linyi, Shandong Province. The SD1291 could only be with primary alveolar macrophages (PAMs), not Marc-145 cells. In addition, 2022 isolate higher vitro replication efficacy 2014 HLJB1...
Porcine parvoviruses (PPVs) are prevalent in China associating with reproductive failure swine. The coinfection of seven genotypes PPVs (PPV1-7) might have synergistic effects on PPV1 associated SMEDI syndrome.
Abstract The prevalence of porcine reproductive and respiratory syndrome virus 1 (PRRSV1) isolates has continued to increase in Chinese swine herds recent years. However, no effective control strategy is available for PRRSV1 infection China. In this study, we generated the first infectious cDNA clone (rHLJB1) a isolate subsequently used it as backbone construct an ORF2-6 chimeric (ORF2-6-CON). This contained synthesized consensus sequence gene encoding all envelope proteins. shared > 90%...
ABSTRACT Porcine reproductive and respiratory syndrome virus (PRRSV) isolates share a restricted cellular tropism. Marc-145 cells derived from African green monkey are one of the few cell lines supporting PRRSV propagation in vitro commonly used for PRRS vaccine development. However, currently prevalent display different tropism while exact determinant is not clarified yet. In this study, we identified first time that 91/97/98 amino acid (aa) substitutions GP2a play critical roles...
Porcine reproductive and respiratory syndrome virus (PRRSV) causes huge economic loss to the global swine industry. Even though several control strategies have been applied, PRRS is still not effectively controlled due continuous emergence of new variants limited cross-protection by current vaccines. During routine epidemiological investigation in 2017, two PRRSV were identified from a severe abortion farm clinically healthy farm, respectively. The viruses isolated denominated as XJ17-5...
Abstract Due to the substantial genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV), commercial PRRS vaccines fail provide sufficient cross protection. Previous studies have confirmed existence PRRSV broadly neutralizing antibodies (bnAbs). However, bnAbs are rarely induced by either natural infection or vaccination. In this study, we designed synthesized a consensus sequence PRRSV2 ORF2-6 genes (ORF2-6-CON) encoding all envelope proteins based on 30...
Porcine epidemic diarrhea virus (PEDV) and porcine reproductive respiratory syndrome (PRRSV) coinfection occurs commonly can synergistically reduce feed intake pig growth. Vaccination is an effective strategy utilized for PED PRRS control, systemic homologous neutralizing antibodies (shnAbs) in serum are used protective efficacy evaluation of vaccines.
RNA isolation with in a high quantity is basic analytical method plant genetics, molecular biology and related physiological investigations. To understand the genetic of Chinese fir, sufficient high-quality total must be obtained for cDNA library construction other downstream applications. However, extracting from fir difficult often requires modification existing protocols. tissues containing large amounts polysaccharides polyphenol compounds are one most isolation. Therefore, we developed...
Multiple commercial porcine reproductive and respiratory syndrome (PRRS) modified live vaccines are currently utilized in Chinese swine herds due to the limited cross-protection of coexistence different PRRS viruses. In this study, an infectious cDNA clone highly pathogenic (HP-PRRS) vaccine JXA1-R strain was generated. We successfully rescued virus from direct vitro DNA transfection rJXA1-R clone, which has similar growth kinetics parental Marc-145 cells. To further evaluate potential use...
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Influenza A virus (IAV) can rapidly disseminate among animals through various transmission routes, with emerging evidence suggesting the ocular surface as an important entrance. However, it remains unclear how invades respiratory tract after exposure. Here, we demonstrated that H1N1 (A/swine/Guangdong/1/2011) utilizes nasolacrimal system to spread from in porcine model. In vivo and ex , IAV could efficiently attach replicate conjunctiva epithelium, which has abundance of α ‐2,6‐linked...