A5057961068- Cystic Fibrosis Research Advances
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Dysphagia Assessment and Management
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Pediatric health and respiratory diseases
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Cell Image Analysis Techniques
- Clostridium difficile and Clostridium perfringens research
- Hernia repair and management
- Vibrio bacteria research studies
- Heme Oxygenase-1 and Carbon Monoxide
- Infant Health and Development
- Parasites and Host Interactions
- Geographic Information Systems Studies
- Infant Nutrition and Health
- Research on Leishmaniasis Studies
- Colorectal Cancer Surgical Treatments
- Gene Regulatory Network Analysis
- Toxoplasma gondii Research Studies
- CRISPR and Genetic Engineering
- Semantic Web and Ontologies
Dartmouth College
2016-2025
Division of Parasitic Diseases and Malaria
2015
Centers for Disease Control and Prevention
2014-2015
Toxoplasma gondii is a ubiquitous parasite that can cause neurologic and ocular disease. We tested sera from 7,072 people ≥ 6 years of age in the 2009–2010 National Health Nutrition Examination Survey (NHANES) for immunoglobulin G antibodies compared these results with two previous NHANES studies. The overall T. antibody seroprevalence among persons was 13.2% (95% confidence limit [CL] 11.8%, 14.5%) age-adjusted 12.4% CL 11.1%, 13.7%); women 15–44 9.1% 7.2%, 11.1%). In U.S. born 12–49 age,...
Cross-experiment comparisons in public data compendia are challenged by unmatched conditions and technical noise. The ADAGE method, which performs unsupervised integration with denoising autoencoder neural networks, can identify biological patterns, but because models, like many over-parameterized, different models perform equally well. To enhance model robustness better build signatures consistent pathways, we developed an ensemble (eADAGE) that integrated stable across models. We applied...
The composition of the gut microbiome in persons with CF is correlated lung health outcomes, a phenomenon referred to as gut-lung axis. Here, we demonstrate that intestinal microbe Bacteroides decreases inflammation through production short-chain fatty acid propionate. Supplementing levels an animal model associated reduced systemic and reduction relative abundance opportunistically pathogenic group Escherichia / Shigella gut. Taken together, these data key role for microbially produced...
There is growing evidence for a link between gastrointestinal bacterial communities and airway disease progression in CF. We demonstrate that infants with CF ≤1 year of age show distinct stool microbiota versus control comparable age. detected associations the gut microbiome exacerbation events cohort CF, vitro studies provided one possible mechanism this observation. These data clarify current therapeutics do not establish like healthy infants, we suggest interventions direct closer to...
Strongyloides stercoralis is a widely distributed parasite that infects 30 to 100 million people worldwide. In the United States strongyloidiasis recognized as an important infection in immigrants and refugees. Public health commercial reference laboratories need simple reliable method for diagnosis of identify treat cases prevent transmission. The laboratory test choice detection disease specific antibodies, most commonly using crude extract IgG antibodies. Recently, luciferase tagged...
Cystic fibrosis (CF) patients chronically infected with both Pseudomonas aeruginosa and Staphylococcus aureus have worse health outcomes than who are monoinfected either P. or S. We showed previously that mucoid strains of can coexist aureusin vitro due to the transcriptional downregulation several toxic exoproducts typically produced by aeruginosa, including siderophores, rhamnolipids, HQNO (2-heptyl-4-hydroxyquinoline N-oxide). Here, we demonstrate exogenous alginate protects from in...
ABSTRACT Cystic fibrosis (CF) is a heritable disease that causes altered physiology at mucosal sites; these changes result in chronic infections the lung, significant gastrointestinal complications as well dysbiosis of gut microbiome, although latter has been less explored. Here, we describe longitudinal development microbiome cohort children with CF (cwCF) from birth through early childhood (0–4 years age) using 16S rRNA gene amplicon sequencing stool samples surrogate for microbiota....
ABSTRACT The healthy human infant gut microbiome undergoes stereotypical changes in taxonomic composition between birth and maturation to an adult-like stable state. During this time, extensive communication microbiota the host immune system contributes health status later life. Although there are many reported associations compositional alterations disease adults, less is known about how development altered pediatric diseases. One linked cystic fibrosis (CF), a multi-organ genetic involving...
ABSTRACT CRISPR (clustered regularly interspaced short palindromic repeat)-Cas (CRISPR-associated protein) systems are diverse and found in many archaea bacteria. These have mainly been characterized as adaptive immune able to protect against invading mobile genetic elements, including viruses. The first step this protection is acquisition of spacer sequences from the invader DNA incorporation those into array, termed adaptation. Progress understanding mechanisms requirements adaptation has...
ABSTRACT The healthy human infant gut microbiome undergoes stereotypical changes in taxonomic composition between birth and maturation to an adult-like stable state. During this time, extensive communication microbiota the host immune system contributes health status later life. Although there are many reported associations compositional alterations disease adults, less is known about how development altered pediatric diseases. One linked cystic fibrosis (CF), a multi-organ genetic involving...
ABSTRACT Persons with cystic fibrosis, starting in early life, have intestinal microbiome dysbiosis characterized part by a decreased relative abundance of the genus Bacteroides . is major producer short chain fatty acid (SCFA) propionate. We demonstrate here that CFTR-/- Caco-2 epithelial cells are responsive to anti-inflammatory effects Furthermore, isolates inhibit IL-1β-induced inflammatory response and do so propionate-dependent manner. also produce low levels butyrate; this SCFA...
Abstract Cross experiment comparisons in public data compendia are challenged by unmatched conditions and technical noise. The ADAGE method, which performs unsupervised integration with neural networks, can effectively identify biological patterns, but because models, like many over-parameterized, different models perform equally well. To enhance model robustness better build signatures consistent pathways, we developed an ensemble (eADAGE) that integrated stable across models. We applied...
Currently, no dressings are utilized after removal of polyps during a colonoscopy rendering these tissue sites susceptible to bleeding, sepsis, and perfusion. We report the design specifications, synthesis, ex vivo evaluation in situ polymerized hydrogels as colon wound post polypectomy. The exhibited varied properties include moduli between 100 16 000 Pa, dissolution times 4 h 7 days or longer, swelling up 200%, adhesion from 0.1 0.4 N/cm2. displayed minimal cytotoxicity, prevented...
ABSTRACT Cystic Fibrosis is a heritable disease that causes altered physiology at mucosal sites; these changes result in chronic infection the lung, significant gastrointestinal complications as well dysbiosis of gut microbiome, although latter has been less explored. Here, we describe longitudinal development microbiome cohort children with cystic fibrosis (cwCF) from birth through early childhood (0-4 years age) using 16S rRNA gene amplicon sequencing stool samples surrogate for...
ABSTRACT Cystic fibrosis (CF) patients chronically infected with both Pseudomonas aeruginosa and Staphylococcus aureus have worse health outcomes than who are mono-infected either P. or S. . We showed previously that mucoid strains of can co-exist in vitro due to transcriptional downregulation several toxic exoproducts typically produced by , including siderophores, rhamnolipids, HQNO (2-heptyl-4-hydroxyquinoline N-oxide). Here we demonstrate exogenous alginate protects from planktonic...
Abstract The gut-lung axis describes the interaction between microbiota in gut and health status of airway, whereby there is a bidirectional relationship facilitated by systemic transport microbially-derived metabolites immune factors. Cystic fibrosis (CF) genetic disease that associated with dysbiosis microbiota. Recent literature has shown microbial CF can alter hosts’ inflammatory are distinct compositions children who have low versus high intestinal inflammation. These profiles be used...