A5087333213- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Traditional and Medicinal Uses of Annonaceae
- Enzyme Production and Characterization
- Analytical Chemistry and Chromatography
- Chemical Synthesis and Analysis
- Synthesis of Organic Compounds
- Biochemical and Molecular Research
- Trypanosoma species research and implications
- Chemical synthesis and alkaloids
- Phytochemical compounds biological activities
- Synthetic Organic Chemistry Methods
- Natural product bioactivities and synthesis
- Natural Antidiabetic Agents Studies
- Diet, Metabolism, and Disease
- Fluorine in Organic Chemistry
- Alkaloids: synthesis and pharmacology
- Polyamine Metabolism and Applications
- Biochemical Analysis and Sensing Techniques
- Phytochemical Studies and Bioactivities
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolites in Food Biotechnology
University of Toyama
2016-2025
Toyama University Hospital
2016-2025
Qatar University
2023
Forestry and Forest Products Research Institute
1990-2022
Toyama College
2015-2019
Kobe Pharmaceutical University
1997-2018
Nissan (Japan)
2017
University of Aveiro
2016
Université de Poitiers
2015
National Institute of Infectious Diseases
2013-2015
New polyhydroxylated alkaloids, (2R,3R,4R)-2-hydroxymethyl-3,4-dihydroxypyrrolidine-N-propionamide from the root bark of Morus alba L., and 4-O-alpha-D-galactopyranosyl-calystegine B(2) 3 beta,6 beta-dihydroxynortropane fruits, were isolated by column chromatography using a variety ion-exchange resins. Fifteen other alkaloids also isolated. 1-Deoxynojirimycin, potent alpha-glucosidase inhibitor, was concentrated 2.7-fold silkworms feeding on mulberry leaves. Some contained in leaves...
Fabry disease is a lysosomal storage disorder caused by deficient alpha-galactosidase A (alpha-Gal A) activity. Deficiency of the enzyme activity results in progressive deposition neutral glycosphingolipids with terminal alpha-galactosyl residue vascular endothelial cells. We recently proposed chemical chaperone therapy for this administration 1-deoxygalactonojirimycin, potent inhibitor enzyme, at subinhibitory intracellular concentrations [Fan, J.-Q., Ishii, S., Asano, N. and Suzuki, Y....
Matricaria chamomilla L., known as "chamomile", has been used an herbal tea or supplementary food all over the world. We investigated effects of chamomile hot water extract and its major components on prevention hyperglycemia protection improvement diabetic complications in diabetes mellitus. Hot extract, esculetin (3) quercetin (7) have found to show moderate inhibition sucrase with IC50 values 0.9 mg/mL 72 71 microM, respectively. In a sucrose-loading test, administration (50 mg/kg body...
l-Enantiomers of 1-deoxynojirimycin (DNJ), 1-deoxymannojirimycin (manno-DNJ), 1-deoxyallonojirimycin (allo-DNJ), 1-deoxyaltronojirimycin (altro-DNJ), 1-deoxygalactonojirimycin (galacto-DNJ), 1-deoxygulonojirimycin (gulo-DNJ), and 1-deoxyidonojirimycin (ido-DNJ) were prepared according to prior methods for the d-enantiomers. These enantiospecific syntheses established unambiguously absolute configuration naturally occurring DNJ, manno-DNJ, allo-DNJ, altro-DNJ, gulo-DNJ. Although d-DNJ...
A toxicokinetic study was performed using rats to investigate the possible mechanism of 18 acute deaths in Japanese patients with cancer and herpes zoster by interactions new oral antiviral drug, sorivudine (SRV), one 5-fluorouracil (5-FU) prodrugs within 40 days after approval use SRV. Tegafur, an anticancer 5-FU prodrug suggested be used most who died, SRV were orally administered simultaneously once daily. All these died 10 days, whereas given or tegafur alone under same dosage conditions...
The aqueous MeOH extract of the leaves and root Xanthocercis zambesiaca (Leguminosae) eight structurally related nitrogen-containing sugars, fagomine (1), 4-O-beta-D-glucopyranosylfagomine (2), 3-O-beta-D-glucopyranosylfagomine (3), 3-epifagomine (4), 2,5-dideoxy-2,5-imino-D-mannitol (5), castanospermine (6), alpha-homonojirimycin (7), 1-deoxynojirimycin (8) were evaluated for antihyperglycemic effects in streptozotocin (STZ)-diabetic mice. insulin-releasing 1 also investigated. blood...
Natural intoxication of livestock by the ingestion Ipomoea carnea (Convolvulaceae) sometimes occurs in tropical regions world. Polyhydroxylated alkaloids were isolated from leaves, flowers, and seeds poisonous plant characterized. Chromatographic separation leaf extract resulted isolation swainsonine (1), 2-epi-lentiginosine (2), calystegines B1 (3), B2 (4), B3 (5), C1 (6), N-methyl-trans-4-hydroxy-l-proline (7). The contents 1 fresh leaves flowers 0.0029 0.0028%, respectively, whereas 1, 3,...
It has long been thought that iminosugar antiviral activity is a function of inhibition endoplasmic reticulum-resident α-glucosidases, and on this basis, many iminosugars have investigated as therapeutic agents for treatment infection by diverse spectrum viruses, including dengue virus (DENV). However, are glycomimetics possessing nitrogen atom in place the endocyclic oxygen atom, ubiquity glycans host metabolism suggests multiple pathways can be targeted via treatment. Successful patients...
We report on the synthesis and biological evaluation of a series α-1-C-alkylated 1,4-dideoxy-1,4-imino-l-arabinitol (LAB) derivatives. The asymmetric derivatives was achieved by allylic alkylation, ring-closing metathesis, Negishi cross-coupling as key reactions. α-1-C-Butyl-LAB is potent inhibitor intestinal maltase, isomaltase, sucrase, with IC50 values 0.13, 4.7, 0.032 μM, respectively. Matrix-assisted laser desorption ionization time-of-flight mass spectrometric analysis revealed that...
The antiviral activity of UV-4 was previously demonstrated against dengue virus serotype 2 (DENV2) in multiple mouse models. Herein, step-wise minimal effective dose and therapeutic window efficacy studies UV-4B (UV-4 hydrochloride salt) were conducted an antibody-dependent enhancement (ADE) model severe DENV2 infection AG129 mice lacking types I II interferon receptors. Significant survival benefit with 10-20 mg/kg administered thrice daily (TID) for seven days initiation treatment up to 48...
Five calystegins were extracted from the roots of Physalis alkekengi var. francheti (Solanaceae) with hot water and purified to homogeneity by combination a variety ion‐exchange column chromato‐graphies. Their structures have been determined 1 H‐ 13 C‐NMR spectral data, two compounds identified as A 3 B 2 , which isolated Calys‐tegia sepium (Convolvulaceae). Two remaining three found be lα,3α,4β‐trihydroxy‐nor‐tropane 1α,2α,3α,4β‐tetrahydroxy‐nor‐tropane given trivial name 5 respectively....
Abstract Protein aggregation is a common phenomenon. The preparation of highly concentrated protein samples, typically required for biophysical measurements, often involves time consuming and tedious testing solvent conditions improving solubility. Here, in systematic analysis, we have determined the increase solubility upon addition SEP‐tags (solubility enhancement peptide tags) containing, one, three, five lysines or arginines (or six arginines) to either N C terminus our low model...
An examination of the roots Lycium chinense (Solanaceae) has resulted in discovery 14 calystegines, a cycloheptane bearing an amino group and three hydroxyl groups, two polyhydroxylated piperidine alkaloids. Calystegines A 7 B 5 addition to previously known calystegines 3 , 6 1 2 4 C N were isolated determined as 1α,2β,4α‐trihydroxy‐nortropane 1α,2β,4α,7α‐tetrahydroxy‐nortropane, respectively. L. also had polyhydroxytropanes methyl on nitrogen atom, unlike reported nortropane They...
We compared the inhibitory action of gabapentin, which is used to treat neuropathic pain, on mechanical allodynia induced by chemotherapeutic agents, paclitaxel, oxaliplatin, and vincristine, in mice. Single injections vincristine at doses corresponding clinically caused similar intensity. Oral administration gabapentin (30, 100 mg/kg) produced a dose-dependent inhibition paclitaxel but not vincristine. Intrathecal injection 100microg/site) significantly inhibited oxaliplatin Intraplantar...
External control of gene expression is crucial in synthetic biology and biotechnology research applications, commonly achieved using inducible promoter systems. The E. coli rhamnose-inducible rhaBAD has properties superior to more used systems, but marred by transient caused degradation the native inducer, l-rhamnose. To address this problem, 35 analogues l-rhamnose were screened for induction promoter, no strong inducers identified. In configuration, an inducer must bind activate two...
C-2 Fluorinated castanospermines have been synthesized from a well-protected aldehyde precursor and compared as glycosidase inhibitors with castanospermine, 1-epi-castanospermine C-1 fluorinated castanospermines. Whereas lose nearly all...