A5048576308- Electron Spin Resonance Studies
- Hydraulic Fracturing and Reservoir Analysis
- Rock Mechanics and Modeling
- Soil and Unsaturated Flow
- ATP Synthase and ATPases Research
- Vehicle emissions and performance
- Advanced Combustion Engine Technologies
- Lipid Membrane Structure and Behavior
- Advanced NMR Techniques and Applications
- CO2 Sequestration and Geologic Interactions
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Groundwater flow and contamination studies
- Spectroscopy and Quantum Chemical Studies
- Landslides and related hazards
- Drilling and Well Engineering
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Electrokinetic Soil Remediation Techniques
- Aerodynamics and Fluid Dynamics Research
- RNA and protein synthesis mechanisms
- Nanotechnology research and applications
- Biotin and Related Studies
- Geothermal Energy Systems and Applications
- Drug Transport and Resistance Mechanisms
University of Manchester
2023-2025
Czech Academy of Sciences, Institute of Biotechnology
2024
Manchester Academic Health Science Centre
2023-2024
University of Leeds
2019-2023
North China University of Technology
2018
Chiba University
2011-2013
Abstract The β‐barrel assembly machinery (BAM complex) is essential for outer membrane protein (OMP) folding in Gram‐negative bacteria, and represents a promising antimicrobial target. Several conformational states of BAM have been reported, but all obtained under conditions which lack the unique features complexity (OM). Here, we use Pulsed Electron‐Electron Double Resonance (PELDOR, or DEER) spectroscopy distance measurements to interrogate ensemble complex E. coli cells. We show that...
Membrane forces shift the equilibria of mechanosensitive channels enabling them to convert mechanical cues into electrical signals. Molecular tools stabilize and methods capture their highly dynamic states are lacking. Cyclodextrins can mimic tension through sequestering lipids from membranes. Here we probe conformational ensemble MscS by EPR spectroscopy, lipid environment with NMR, function electrophysiology under cyclodextrin-induced tension. We show extent activation depends on...
<title>Abstract</title> SbmA is a membrane transporter from <italic>Escherichia coli</italic> that imports antimicrobial peptides. Although the protein secondary energized by proton gradient, it structurally related to transmembrane domain (TMD) of ATP-binding cassette (ABC) transporters. therefore bridges structural divide between primary and However, remained unclear, if also shares mechanism alternating access with ABC transporters, because only single (outward-open) state has been...
The mechanosensitive channel of large conductance (MscL) from Mycobacterium tuberculosis has been used as a structural model for rationalizing functional observations in multiple MscL orthologs. Although these orthologs adopt similar architectures, they reportedly present significant differences. Subtle discrepancies on nanopockets are known to affect mechanical gating and may be linked variability tension sensitivity among membrane channels. Here, we modify the nanopocket regions...
Solvent accessibilities of and distances between protein residues measured by pulsed-EPR approaches provide high-resolution information on dynamic motions. We describe protocols for the purification site-directed spin labeling integral membrane proteins. In our protocol, peptide-level HDX-MS is used as a precursor to guide single-residue resolution ESEEM accessibility measurements strategies EPR applications. Exploiting pentameric MscL channel model, we discuss use cwEPR, DEER/PELDOR,...
Electron paramagnetic resonance (EPR) spectroscopy is a powerful technique for the study of proteins in solution and under native conditions. Paramagnetic spin centres are usually introduced by site-directed labelling (SDSL) engineered cysteine residues. However, many (membrane) protein classes, engineering not possible without affecting their structural functional integrity. Here, we report development xanthine-based aminoxyl probes that allow non-covalent human TRPC5 ion channels. The...
Membrane-bound pyrophosphatases (mPPases) are homodimeric proteins that hydrolyse pyrophosphate and pump H + /Na across membranes. They crucial for the virulence of protist pathogens, making them attractive drug targets. In this study, we investigate inhibitory effects seven distinct bisphosphonates against Thermotoga maritima mPPase to explore their mode action assist in future small molecule inhibitor development. We solved two structures bound inhibitors enzyme active sites probed...
Modeling of fluids in deformable geoformation media has gained much attention the past decades due to significant applications such as groundwater prediction, and shale gas carbon capture storage. However, considerable research been focused on porous concept, dual network (fracture pores) multiphysics coupled modeling remained a challenge lack systemic theory bridge physical deformation (e.g., rocks) interaction water flow pores fractures. This paper adopts nonequilibrium...
Rock, soil and many porous-like materials are often fractured or structured media, which can exhibit dual-porosity behaviour. Studies on solute transport in deformable media remain challenging due to the multi-physics coupled effects complex interaction between fractures (or macropores) porous matrix. Although several studies exist constitutive modelling of behaviour previously developed models not systematic thermodynamical frameworks. This paper proposes a mixture coupling theory approach...
Abstract The interactions of gas migration, water transport and mechanical deformation rocks are significant for geoenergy industry (e.g. Carbon Capture Storage, radioactive waste disposal); however, the hydro-mechanical-gas coupled model remains a challenge due to gap between multiple disciplines Geomechanics Geoenergy). This work presents novel hydro-mechanical framework fully two-phase fluid in deformable porous media through extending mixture coupling theory which is based on...
Summary Coupled thermo‐hydro‐mechanical‐chemical modelling has attracted attention in past decades due to many contemporary geotechnical engineering applications (e.g., waste disposal, carbon capture and storage). However, molecular‐scale interactions within geomaterials swelling dissolution/precipitation) have a significant influence on the mechanical behaviour, yet are rarely incorporated into existing Thermal‐Hydro‐Mechanical‐Chemical (THMC) frameworks. This paper presents new coupled...
The β-barrel assembly machinery (BAM complex) is essential for outer membrane protein (OMP) folding in Gram-negative bacteria, and represents a promising antimicrobial target. Several conformational states of BAM have been reported, but all obtained under conditions which lack the unique features complexity (OM). Here, we use Pulsed Electron-Electron Double Resonance (PELDOR, or DEER) spectroscopy distance measurements to interrogate ensemble complex
Membrane-bound pyrophosphatases (mPPases) are homodimeric proteins that hydrolyse pyrophosphate and pump H + /Na across membranes. They crucial for the virulence of protist pathogens, making them attractive drug targets. In this study, we investigate inhibitory effects seven distinct bisphosphonates against Thermotoga maritima mPPase to explore their mode action assist in future small molecule inhibitor development. We solved two structures bound inhibitors enzyme active sites probed...
The present study takes the ratio of matric suction to net vertical stress and mean as new unsaturated levels <math xmlns="http://www.w3.org/1998/Math/MathML" id="M1"><mi>f</mi></math> id="M2"><mi>F</mi></math> , respectively. Based on laboratory tests theoretical derivation, modified one-dimensional Mesri creep model three-dimensional were established, which level into account. Then, characteristics viscous subsoil an airport under different analyzed. following conclusions could be drawn:...