A5086608151- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Liver Diseases and Immunity
- Malaria Research and Control
- Liver Disease Diagnosis and Treatment
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Pediatric Hepatobiliary Diseases and Treatments
- Antibiotic Resistance in Bacteria
- Liver Disease and Transplantation
- Vector-borne infectious diseases
- Insect Resistance and Genetics
- Bacterial biofilms and quorum sensing
- Inflammatory Bowel Disease
- Gut microbiota and health
- Vibrio bacteria research studies
- Sarcoma Diagnosis and Treatment
- Diet and metabolism studies
- Computational Drug Discovery Methods
- Bacteriophages and microbial interactions
- Insect Pest Control Strategies
- Ophthalmology and Visual Health Research
- Agricultural risk and resilience
- Eosinophilic Disorders and Syndromes
- Birth, Development, and Health
Safdarjang Hospital
2016-2025
National Institute of Pathology
2016-2025
Indian Council of Medical Research
2006-2025
University of North Carolina at Charlotte
2025
Children's Hospital of Philadelphia
2025
Institute of Child Health
2025
Bharati Vidyapeeth Deemed University
2024
Cincinnati Children's Hospital Medical Center
2019-2023
University of Cincinnati
2023
Jawaharlal Nehru Medical College
2023
Lipopolysaccharide (LPS), a potent endotoxin present in the outer membrane of Gram-negative bacteria, causes chronic immune responses associated with inflammation. In study, association between LPS and dysbiosis bacteria gut microbiome was determined patients type 2 diabetes mellitus (T2DM) kidney disease (T2DM-CKD; stages 4 5, not on dialysis) compared healthy individuals. Microbiome diversity analyzed T2DM-CKD controls by sequencing hypervariable sub-regions 16S ribosomal RNA gene from...
Trimethylamine-N-oxide (TMAO) is a product of dietary, gut microbiome, and tissues metabolism. Elevated blood TMAO levels are associated with heart attack, stroke chronic kidney disease (CKD). The purpose our study was to investigate the microbiota trimethylamine (TMA) production, precursor TMAO, serum inflammatory biomarkers type 2 diabetes mellitus (T2DM) CKD. Twenty adults T2DM advanced CKD 20 healthy participated in study. Analyses included anthropometric metabolic parameters,...
Background Miltefosine (MIL) is an oral antileishmanial drug used for treatment of visceral leishmaniasis (VL) in the Indian subcontinent. Recent reports indicate a significant decline its efficacy with high rate relapse VL as well post kala-azar dermal (PKDL). We investigated parasitic factors apparently involved miltefosine unresponsiveness clinical isolates Leishmania donovani. Methodology L. donovani isolated from patients and PKDL at pretreatment stage (LdPreTx, n = 9), that relapsed...
Saquinavir (SQV) is a US-FDA approved HIV protease inhibitor (HPI) for cure. The purpose of the present investigation was to develop and characterize anticancer potential SQV-loaded folic acid (FA) conjugated PEGylated non-PEGylated poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles (NPs) (SQV-Fol-PEG-PLGA SQV-Fol-PLGA) employing PC-3 (human prostate) MCF-7 breast) cancer cell lines.Developed NPs were characterized by IR, NMR, DSC, XRD, size, charge further tested drug loading cellular...
Background and Aims Many children with primary sclerosing cholangitis (PSC) receive oral vancomycin therapy (OVT) or ursodeoxycholic acid (UDCA). There is a paucity of data on whether these medications improve outcomes. Approach Results We analyzed retrospective from the Pediatric PSC Consortium. Children treated OVT were matched 1:1:1 to those UDCA managed observation (no treatment) based closest propensity score, ensuring similar baseline characteristics. Two hundred sixty‐four patients...
An otherwise healthy 20-year-old woman in Goa, India, received antibiotics after a diagnosis of upper respiratory tract infection. One week later, vivax malaria was diagnosed at health center, but the patient developed distress and lost consciousness. She arrived emergency department shock, breathless, comatose. died within minutes. Two independent laboratories later confirmed Plasmodium by microscopy (140,000/μL) nested real-time polymerase chain reaction methods. Post-mortem examination...
Background Recent studies have shown significant decline in the final cure rate after miltefosine treatment visceral leishmaniasis. This study evaluates efficacy of post kala-azar dermal leishmaniasis (PKDL) patients recruited over a period 5 years with 18 months follow-up. Methodology In this 86 confirmed cases PKDL were treated two different dosage regimens (Regimen I- 50mg twice daily for 90 days and Regimen II- 50 mg thrice 60 days) clinical outcome assessed monthly. Cure/relapse was...
Background: The resistance to colistin and carbapenems in Klebsiella pneumoniae infections have been associated with increased morbidity mortality worldwide. A retrospective observational study was conducted determine the prevalence molecular events contributing resistance. Methods: Clinical samples were screened for underlying mechanisms studied by PCR-based amplification sequence analysis of genes two-component regulatory system ( phoPQ pmrAB ), transmembrane protein-coding mgrB ,...
Indian Leishmania donovani isolates (n = 19) from regional zones representing various levels of antimony resistance displayed significantly (P < 0.01) correlated results with respect to in vitro susceptibility the antileishmanial drugs sodium gluconate, amphotericin B, and Miltefosine, raising possibility cross-resistance mechanisms operating field isolates. The gene expression analysis LdMT LdRos3 were suggestive alternate Miltefosine
Patients with Post kala-azar dermal leishmaniasis (PKDL) are considered a reservoir of Leishmania donovani. It is imperative to identify and treat them early for control visceral (VL), current priority in the Indian subcontinent. We explored trends clinico-epidemiological features PKDL cases over last two decades, improving management disease.Clinically suspected were diagnosed rK39 strip test followed by parasitological confirmation microscopy and/or PCR/qPCR skin tissue/slit aspirates....
Leishmaniasis is a spectrum of diseases with great relevance to public health. Conventional diagnostic methods are time consuming, needing trained personnel. A robust, rapid and cost effective test warranted for on-time diagnosis field application. We have developed loop mediated isothermal amplification (LAMP) assay primers (n = 6) based on Leishmania donovani kDNA detection infection, using closed tube prevent cross-contamination. The was used detect infection in biological samples...
Plasmodium falciparum is an obligate human parasite of the phylum Apicomplexa and causative agent most lethal form malaria. Although N6-methyladenosine modification thought to be one major post-transcriptional regulatory mechanisms for stage-specific gene expression in apicomplexan parasites, precise base position m6A mRNAs or noncoding RNAs these parasites remains unknown. Here, we report global nucleotide-resolution mapping residues P. using DART-seq technology, which quantitatively...
In the current scenario, treatment of Post Kala Azar Dermal leishmaniasis (PKDL) is intravenous liposomal amphotericin B or oral miltefosine. Over years, there has been a gradual decline in efficacy miltefosine and rise permanent loss vision. Moreover, non-endemic states, not freely available. view this, we attempted two step regimen itraconazole to treat patients PKDL diagnosed on histopathology as well PCR. We found this be highly effective with complete recovery.
Severe acute kidney injury (AKI) portends poor outcomes in pediatric sepsis. We evaluated the trajectory and prognostic utility of AKI biomarkers septic shock using a subset participants ongoing Pragmatic Pediatric Trial Balanced vs. Normal Saline Fluid Sepsis (PRoMPT BOLUS) trial, NCT04102371. tested whether fluid volume is associated with persistent elevation urine neutrophil gelatinase-associated lipocalin (Ur-NGAL). Prospective, non-prespecified cohort study within PRoMPT BOLUS trial....
Artemisinin-based combination therapy (ACT) is the treatment of choice for uncomplicated falciparum malaria. Artemether-lumefantrine (AL), a fixed dose co-formulation, has recently been approved marketing in India, although it not included National Drug Policy Efficacy short course regimen (4 × 4 tablets 20 mg artemether plus 120 lumefantrine over 48 h) was demonstrated India year 2000. However, low cure rates Thailand and better plasma concentration profile with six-dose three days, led to...