A5026536004- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neonatal and fetal brain pathology
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic and Kidney Cyst Diseases
- Barrier Structure and Function Studies
- RNA regulation and disease
- Cancer, Hypoxia, and Metabolism
- Vestibular and auditory disorders
- Renal and related cancers
- Circular RNAs in diseases
- Head and Neck Anomalies
- Nanoplatforms for cancer theranostics
- Anesthesia and Neurotoxicity Research
- Hearing, Cochlea, Tinnitus, Genetics
- MicroRNA in disease regulation
University of Sheffield
2015-2021
ABSTRACT The central nervous system (CNS) has specific barriers that protect the brain from potential threats and tightly regulate molecular transport. Despite critical functions of CNS barriers, mechanisms underlying their development function are not well understood, there very limited experimental models for study. Claudin 5 is a tight junction protein required blood barrier (BBB) and, probably, choroid plexus (CP) structure in vertebrates. Here, we show gene claudin 5a zebrafish...
Abstract Background This study aimed to explore the biological activities of miR-330-3p in dextan sulphate sodium (DSS)-induced ulcerative colitis and apoptosis direct target this process. HT-29 cells male C57BL/6 mice were used examine function vitro vivo, respectively. Expression miRNA mRNA was measured using quantitative real time PCR (qRT-PCR). Western blotting measure change protein expression. Flow cytometry determine cell luciferase assay confirm miR-330-3p. Results expression...
Significance We found that genes associated with human ciliopathies modulate apicobasal polarity of secretion. In addition, we showed this is essential for the function Cochlin, protein DFNA9, a nonsyndromic deafness vestibular dysfunction. Cochlin secreted by group specialized epithelial cells both apically and basally in ear. proper otolith formation. way, offer plausible molecular explanation as to why DFNA9 patients suffer from balance disorders. The involvement cilia polarized secretion...
ABSTRACT The central nervous system (CNS) has specific barriers that protect the brain from potential threats and tightly regulate molecular transport. Despite critical functions of CNS barriers, mechanisms underlying their development function are not well understood, there very limited experimental models for study. Claudin 5 is a tight junction protein required blood barrier (BBB) choroid plexus (CP) structure in humans. Here, we show gene claudin 5a zebrafish orthologue with high...
In vertebrate photoreceptors, opsins are highly concentrated in a morphologically distinct ciliary compartment known as the outer segment (OS). Opsin is synthesized cell body and transported to OS at remarkable rate of 100 1000 molecules per second. transport defects contribute photoreceptor loss blindness human ciliopathies. Previous studies revealed that rhodopsin C-terminal tail, 44 amino acids, sufficient mediate targeting Xenopus photoreceptors. Here, we show that, although C-terminus...
Vision begins as photons are captured by photoreceptor cilia and light is converted into electrical signals that then sent to the brain. As cilium not able make its own proteins, all polypeptides needed for converting synthetized in cell body. How these molecules move from body still unclear. Opsin one of best-characterized transmembrane proteins. Our goal understand mechanism opsin transport cilia. In this project, we use a combination genetic proteomic approaches zebrafish model. first...
Objective To investigate the influence of in vitro artificial CO2 cavity on matrix metalloproteinase 2(MMP-2), adhesion molecule vascular cell 1(VCAM-1), and intercellular 1(ICAM-1)expression MDA-MB-231 cell. Methods An model was established. cells were exposed to under pressure 7 mmHg for 1, 2 4 hours, respectively. MMP-2 concentration measured by enzyme linked immunosorbent assay(ELISA). VCAM-1 ICAM-1 expression flow cytometry 0, 24, 48 72 hours after CO2-insufflation. Hypoxia group...