A5064883065- Zebrafish Biomedical Research Applications
- Genetic and Kidney Cyst Diseases
- Aquaculture disease management and microbiota
- Viral Infections and Vectors
- Protist diversity and phylogeny
- interferon and immune responses
- Pneumonia and Respiratory Infections
- Erythrocyte Function and Pathophysiology
- Fetal and Pediatric Neurological Disorders
- RNA Research and Splicing
- Hereditary Neurological Disorders
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Retinal Development and Disorders
- Nerve injury and regeneration
- Microtubule and mitosis dynamics
- Phagocytosis and Immune Regulation
- Developmental Biology and Gene Regulation
- Neurogenetic and Muscular Disorders Research
- Ubiquitin and proteasome pathways
- Mechanical Circulatory Support Devices
- Photoreceptor and optogenetics research
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
Jagiellonian University
2020-2025
University of Sheffield
2016-2022
In mammals, the relationship between immune system and behavior is widely studied. fish, however, knowledge concerning brain response behavioral changes during viral infection very limited. To further investigate this subject, we used model of tilapia lake virus (TiLV) zebrafish ( Danio rerio ), which was previously developed in our laboratory. We demonstrated that TiLV persists adult for at least 90 days, even when not detectable other peripheral organs. The virions were found whole brain....
Streptococcus pneumoniae is an opportunistic pathogen responsible for life-threatening diseases including pneumonia and meningitis. The host defense against pneumococci relies heavily on macrophages, which can effectively internalize degrade bacteria. Recent studies have implicated both canonical non-canonical autophagy-related processes in bacterial clearance, but the precise pathways mediating S. remain unknown. Here, we utilize a well-established zebrafish larval infection model to...
Abstract Transport of proteins in the ciliary shaft is driven by microtubule-dependent motors, kinesins. Prior studies suggested that heterotrimeric kinesin may be dispensable for certain aspects transport specialized cilia vertebrate photoreceptor cells. To test this possibility further, we analyzed mutant phenotype zebrafish kif3a gene, which encodes common motor subunit Cilia are absent all organs examined, leading to conclusion indispensable ciliogenesis cells, including photoreceptors....
Tilapia lake virus (TiLV; genus: Tilapinevirus, family: Amnoonviridae) is a recently characterised enveloped with linear, negative-sense single-stranded RNA genome, which causes high mortality in tilapia species. In the present study, we demonstrated that zebrafish (Danio rerio) larvae are susceptible to TiLV infection upon systemic injection. replicated and caused their (of about 70%). Histopathological examination revealed pathological abnormalities were well visible within brain....
Cilia are evolutionarily highly conserved organelles with important functions in many organs. The extracellular component of the cilium protruding from plasma membrane comprises an axoneme composed microtubule doublets, arranged a 9 + 0 conformation primary cilia or 2 motile cilia. These microtubules facilitate transport intraflagellar cargoes along axoneme. They also provide structural stability to cilium, which may play role sensory cilia, where signals received movement fluid....
Abstract Charcot Marie Tooth Disease (CMT) is a group of inherited progressive conditions affecting distal motor and sensory neurons, leading to muscle weakness, pain loss sensation in limbs. There are currently no treatments for this debilitating disease. To investigate disease mechanisms facilitate treatment discovery, here we developed an vitro model CMT type 2A by introducing the patient-specific MFN2 R94Q mutation into human embryonic stem cells (hESCs). Isogenic mutant wild-type hESCs...
Abstract Monocyte chemoattractant protein-induced protein 1 (MCPIP1), also called Regnase-1, is an RNase that has been described as a key negative modulator of inflammation. MCPIP1 controls numerous tumor-related processes, such proliferation, apoptosis and differentiation. In this study, we utilized zebrafish model to investigate the role Mcpip1 during embryogenic development. Our results demonstrated embryogenesis, expression zc3h12a gene encoding undergoes dynamic changes. Its transcript...
Streptococcus pneumoniae (the pneumococcus) is a major human pathogen causing invasive disease, including community-acquired bacteraemia, and remains leading cause of global mortality. Understanding the role phagocytes in killing bacteria still limited, especially vivo. In this study, we established zebrafish model to study interaction between intravenously administered pneumococci professional such as macrophages neutrophils, unravel bacterial mechanisms employed by these immune cells. Our...