A5045904954- Animal Genetics and Reproduction
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Liver physiology and pathology
- RNA and protein synthesis mechanisms
- Renal cell carcinoma treatment
- Xenotransplantation and immune response
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Reproductive Biology and Fertility
- Renal and related cancers
- Immune Response and Inflammation
- Ferroptosis and cancer prognosis
- Dermatology and Skin Diseases
- Endoplasmic Reticulum Stress and Disease
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Liver Diseases and Immunity
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Cell death mechanisms and regulation
- Genetically Modified Organisms Research
Jagiellonian University
2016-2025
National Research Institute of Animal Production
1994-2018
General Department of Preventive Medicine
2013
Zakład Doświadczalny Instytutu Zootechniki
1987-2006
Polish Academy of Sciences
2000
In human monocyte-derived macrophages, the MCPIP gene (monocyte chemoattractant protein-induced protein) is strongly activated by interleukin-1beta (IL-1beta). Using bioinformatics, a PIN domain was identified, spanning amino acids 130-280; such domains are known to possess structural features of RNases. Recently, RNase properties were confirmed on transcripts coding for interleukins IL-6 and IL-12p40. Here we present evidence that siRNA-mediated inhibition expression increases level...
A novel gene ZC3H12A , encoding MCP‐1‐induced protein 1 (MCPIP), was recently identified in human peripheral blood monocytes treated with monocyte chemotactic (MCP‐1) and monocyte‐derived macrophages stimulated interleukin (IL)‐1β. These experiments revealed that the undergoes rapid potent transcription induction upon stimulation proinflammatory molecules, such as MCP‐1, IL‐1β, tumour necrosis factor α lipopolysaccharide. Here we show of by IL‐1β is predominantly NF‐κB‐dependent because...
Monocyte Chemoattractant protein-induced protein 1 (MCPIP1), also known as Regnase-1, is encoded by the ZC3H12a gene, and it mediates inflammatory processes regulating stability of transcripts coding for proinflammatory cytokines controlling activity transcription factors, such NF-κB AP1. We found that MCPIP1 transcript levels are strongly downregulated in clear cell renal carcinoma (ccRCC) samples, which were derived from patients surgically treated cancer compared to surrounded normal...
Abstract Clear cell renal carcinoma (ccRCC) is the most common type of kidney cancer and it forms highly vascularized tumors. The monocyte endoribonuclease MCPIP1 negatively regulates inflammation by degrading mRNA encoding proinflammatory cytokines, such as IL6, IL1, IL12. also a negative regulator NFκB AP1 activity influences broad range miRNA activities. Here we report that protein levels are decreased during progression. In patient-derived tumors xenografts established in NOD-SCID or...
MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The expression gene coding for - ZC3H12A poorly explored. Here we report that proinflammatory cytokine IL-1β rapidly induces synthesis primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through MAP kinase pathway following activation transcription factor Elk-1. Using reporter construct have shown promoter region, stretching from -76 to +60, mediates...
Abstract: The platinum (Pt)-group elements (PGEs) represent a new kind of environmental pollutant and hazard for human health. Since their introduction as vehicle-exhaust catalysts, emissions into the environment have grown considerably compared with low natural concentration in earth crust. PGE from vehicle catalysts can be also form nanometer-sized particles (Pt nanoparticles [PtNPs]). These elements, both metallic or ions solubilized biological media, are now recognized potent allergens...
Background & AimsHepatic fibrosis is characterized by enhanced deposition of extracellular matrix (ECM), which results from the wound healing response to chronic, repeated injury any etiology. Upon injury, hepatic stellate cells (HSCs) activate and secrete ECM proteins, forming scar tissue, leads liver dysfunction. Monocyte-chemoattractant protein-induced protein 1 (MCPIP1) possesses anti-inflammatory activity, its overexpression reduces in septic mice. In addition, mice with liver-specific...
Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape type of carrier, AgNPs demonstrate different physical chemical properties. strong antimicrobial, antiviral antifungal activity, thus they are used extensively a range medical settings, particularly wound dressings but also cosmetics. This study was undertaken to examine potential toxic effects 15 nm polyvinylpyrrolidone-coated primary normal human...
Pattern recognition receptors are critical for the detection of invading microorganisms. They activate multiple pathways that lead to induction proinflammatory responses and pathogen clearance. The intensity duration this immune reaction must be tightly controlled spatially temporally in every tissue by different negative regulators. We hypothesized monocyte chemoattractant protein-1-induced protein-1 (MCPIP-1) might play a role maintaining homeostasis epithelium both under physiological...
Abstract Tyrosine kinase inhibitors (TKIs) are the most commonly used targeted therapeutics in clear-cell renal cell carcinoma (ccRCC); however, drug resistance limits their utility and can lead to tumor “flare-up” progression. In this study, we show that RCC sunitinib sorafenib involves different mechanisms leads increased malignancy. Sunitinib decreased growth motility along with E-cadherin expression secretion of proangiogenic cytokines IL6 IL8, which activated senescence ccRCC cells led...
Abstract The RNase activity of MCPIP1 is essential for regulating cellular homeostasis, proliferation, and tumorigenesis. Our study elucidates the effects downregulation expression an RNase-inactivating mutation (D141N) on normal epithelial kidney cells, indicating that a key factor suppresses neoplastic transformation. We observed either or significantly increased its clonogenicity altered cancer stem cell (CSC) markers factors involved in epithelial-to-mesenchymal transition (EMT). In vivo...
There is a growing interest in the genetic variation of noncoding genomic elements, including miRNAs, and several mutations miRNA genes implicated human diseases, cancer, have already been detected. However, lack dedicated analytical tools severely hampers progress this area. In study, we developed whole-miRNome sequencing (WMS), which enables targeted all (n~2000) 28 biogenesis genes. Herein, by almost 600 samples, ~300 tumor/normal pairs samples from different cancer types, identified...
Abstract Background Monocyte chemoattractant protein-induced protein 3 (MCPIP3), also called Regnase-3 and encoded by the ZC3H12C gene, is a member of MCPIP family RNases. Previous studies showed that MCPIP1 in keratinocytes plays pivotal role maintenance skin integrity immunological function. Given expression MCPIP3, similar to MCPIP1, increased psoriatic lesions compared with uninvolved skin, MCPIP3 regulation keratinocyte epidermal biology was hypothesized. Methods This study aimed...
MCPIP1, also known as Regnase-1, is a ribonuclease crucial for regulation of stability transcripts related to inflammatory processes. Here, we report that MCPIP1 acts an endonuclease by degrading several stem-loop RNA structures and single-stranded RNAs. Our studies revealed cleavage sites present in the stem-loops derived from 3' untranslated region interleukin-6 transcript. Furthermore, induced at loop motif structures. Additionally, observed could cleave fragments. However, substrates...
ZC3H12B is the most enigmatic member of ZC3H12 protein family. The founding this family, Regnase-1/MCPIP1/ZC3H12A, a well-known modulator inflammation and involved in degradation inflammatory mRNAs. In study, for first time, we characterized properties protein. We show that biological role depends on an intact NYN/PIN RNase domain. Using RNA immunoprecipitation, experiments utilizing actinomycin D ELISA, binds interleukin-6 ( IL-6 ) mRNA vivo, regulates its turnover, results reduced...
Abstract Epithelial-mesenchymal transition (EMT) refers to the acquisition of mesenchymal properties in cells participating tumor progression. One hallmark EMT is increased level active β-catenin, which can trigger transcription Wnt-specific genes responsible for control cell fate. We investigated how Monocyte Chemotactic Protein-1-Induced Protein-1 (MCPIP1), a negative regulator inflammatory processes, affects clear renal carcinoma (ccRCC) line, patient tissues and xenotransplant model....
The recently discovered MCPIP1 (monocyte chemoattractant protein-induced protein 1), a multidomain encoded by the (ZC3H12A) gene, has been described as new differentiation factor, ribonuclease, and deubiquitination-supporting factor. However, its role in cancer is poorly recognized. Our recent analysis of microarrays data showed lack expression transcript primary neuroblastoma, most common extracranial solid tumor children. Additionally, enforced gene BE(2)-C cells caused significant...