A5073613815- Nuclear Structure and Function
- RNA Research and Splicing
- Muscle Physiology and Disorders
- MicroRNA in disease regulation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Polish Historical and Cultural Studies
- Congenital limb and hand anomalies
- Cancer-related molecular mechanisms research
- Central European Literary Studies
- Heat shock proteins research
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Cellular Mechanics and Interactions
- Neurogenetic and Muscular Disorders Research
- Romani and Gypsy Studies
- Muscle activation and electromyography studies
- Digestive system and related health
- Virus-based gene therapy research
- Skin and Cellular Biology Research
- Tissue Engineering and Regenerative Medicine
- Parvovirus B19 Infection Studies
- Bone and Dental Protein Studies
- Nanoparticle-Based Drug Delivery
- ATP Synthase and ATPases Research
University of Wrocław
2014-2025
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2022-2024
Google (United States)
2015
Abstract A growing body of evidence indicates an important role miRNAs in cancer; however, there is no definitive, convenient-to-use list cancer-related or miRNA genes that may serve as a reference for analyses cancer. To this end, we created 165 called the Cancer Census (CMC). The based on score, built various types functional and genetic particular cancer, e.g. miRNA–cancer associations reported databases, with cancer hallmarks, signals positive selection alterations presence...
Abstract Background Maspin, which is classified as a tumor suppressor protein, downregulated in many types of cancer. Several studies have suggested potential anti-proliferative activity maspin well sensitizing for therapeutic cytotoxic agents breast cancer tissue culture and animal models. All the experimental data gathered so far been based on with localized cytoplasmically, while cells may be located cytoplasm, nucleus or both. In this study, effect cytoplasmic nuclear location expression...
Emery-Dreifuss muscular dystrophy type 1 (EDMD1) is a rare genetic disease caused by mutations in the EMD gene, which encodes nuclear envelope protein emerin. Despite understanding basis of disease, molecular mechanism underlying muscle and cardiac pathogenesis remains elusive. Progress restricted limited availability patient-derived samples; therefore, there an urgent need for human-specific cellular models. In this study, we present generation characterization induced pluripotent stem cell...
There is a growing interest in the genetic variation of noncoding genomic elements, including miRNAs, and several mutations miRNA genes implicated human diseases, cancer, have already been detected. However, lack dedicated analytical tools severely hampers progress this area. In study, we developed whole-miRNome sequencing (WMS), which enables targeted all (n~2000) 28 biogenesis genes. Herein, by almost 600 samples, ~300 tumor/normal pairs samples from different cancer types, identified...
LMNA gene encodes for nuclear intermediate filament proteins lamin A/C. Mutations in this lead to a spectrum of genetic disorders, collectively referred as laminopathies. Lamin A/C are widely expressed most differentiated somatic cells but not early embryos and some undifferentiated cells. To investigate the role cell phenotype maintenance differentiation, which could be determinant pathogenesis laminopathies, we examined played by exogenous A its mutants lines (HeLa, NHDF)...
Gene therapy is a promising strategy to cure rare diseases. The lack of regulatory sequences ensuring specific and robust expression in skeletal cardiac muscle substantial limitation gene efficiency targeting the tissue. Here we describe novel hybrid (MH) promoter that highly active both cells. It has an easily exchangeable modular structure, including intronic module enhances driven by it. In cultured myoblasts, myotubes, cardiomyocytes, MH gives relatively stable as well higher activity...
Emerin is an essential LEM (LAP2, Emerin, MAN1) domain protein in metazoans and integral membrane associated with inner outer nuclear membranes. Mutations the human EMD gene coding for emerin result rare genetic disorder: Emery–Dreifuss muscular dystrophy type 1 (EDMD1). This disease belongs to a broader group called laminopathies—a heterogeneous of disorders affecting tissues mesodermal origin. EDMD1 phenotype characterized by progressive muscle wasting, contractures elbow Achilles tendons,...
The taxanes are commonly used in the treatment of many types cancer. disadvantages using therapy their low solubility water, toxicity or relatively poor pharmacokinetics existing formulations. Using liposomes as carriers would help overcoming these problems, however, use is limited by incorporation efficiency taxane molecules within bilayer and subsequent drug crystallization. Most published liposomal formulations natural soy phosphatidylcholine (PC) main lipid. This allows a good retention...
Abstract Xenopus LAP2β protein is the single isoform expressed in XTC cells. The localizes on heterochromatin clusters both at nuclear envelope and inside a cell nucleus. majority of XLAP2β fraction neither colocalizes with TPX2 during interphase nor can be immunoprecipitated antibody. Knockdown expression cells by synthetic siRNA plasmid encoded resulted abnormalities including changes shape nuclei, abnormal chromatin structure, loss envelope, mislocalization integral membrane proteins INM...
ABSTRACT Lamins and topoisomerases have been studied as major karyoskeletal proteins since early 80-ties. Today, lamins are thought to be responsible not only for maintenance structural support of cell nuclei but also involved, directly or indirectly, in regulation chromatin structure, distribution, gene expression, splicing, transcription transport. Topoisomerases involved chromosomal scaffold functions topology DNA, play an important role transcription. They necessary mitosis. For studying...
Emery-Dreifuss muscular dystrophy type 1 (EDMD1) is a rare genetic disease caused by mutations in the EMD gene, which encodes nuclear envelope protein emerin. Despite understanding basis of disease, molecular mechanism underlying muscle and cardiac pathogenesis remains elusive. Progress restricted limited availability patient-derived samples, therefore there an urgent need for human-specific cellular models. In this study, we present generation characterization induced pluripotent stem cell...
Emery-Dreifuss muscular dystrophy type 1 (EDMD1) is a rare genetic disease caused by mutations in the EMD gene coding for nuclear envelope protein emerin. We generated and characterized induced pluripotent stem cells (iPSCs) from two EDMD1 patients bearing mutation c.del153C one healthy donor. That leads to generation of premature STOP codon. Established iPSCs are very valuable tool pathogenesis investigation development new therapeutic methods after differentiation cardiac or muscle cells....