A5037785815- Aquaculture disease management and microbiota
- interferon and immune responses
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Invertebrate Immune Response Mechanisms
- Viral Infections and Vectors
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Aquaculture Nutrition and Growth
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Sperm and Testicular Function
- RNA Research and Splicing
- Toxoplasma gondii Research Studies
- Reproductive biology and impacts on aquatic species
- Agriculture, Plant Science, Crop Management
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- Fish Ecology and Management Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Turtle Biology and Conservation
- Chemokine receptors and signaling
- Cancer-related molecular mechanisms research
Jagiellonian University
2016-2025
University of Liège
2011-2022
The Farah Hospital
2017
Institut de Biologia Evolutiva
2017
Polish Academy of Sciences
2003-2014
Wageningen University & Research
2003-2008
Bipar
2008
Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a mortal and highly contagious disease in common koi carp. The skin major portal entry CyHV-3 carp after immersion water containing virus. In present study, we used vivo bioluminescence imaging to investigate effect mucus removal epidermis lesion on entry. Physical treatments inducing up complete erosion were applied defined area just before inoculation by infectious water. was drastically enhanced where removed with or without...
Both endotherms and ectotherms (e.g., fish) increase their body temperature to limit pathogen infection. Ectotherms do so by moving warmer places, hence the term "behavioral fever." We studied manifestation of behavioral fever in common carp infected cyprinid herpesvirus 3, a native pathogen. Carp maintained at 24°C died from infection, whereas those housed multi-chamber tanks encompassing 24°C–32°C gradient migrated transiently warmest compartment survived as consequence. Behavioral...
Cyprinid herpesvirus 3 (CyHV-3) is causing severe economic losses worldwide in common and koi carp industries, a safe efficacious attenuated vaccine compatible with mass vaccination needed. We produced single deleted recombinants using prokaryotic mutagenesis. When producing recombinant lacking open reading frame 134 (ORF134), we unexpectedly obtained clone additional deletion of ORF56 ORF57. This triple replicated efficiently vitro expressed an vivo safety/efficacy profile use as vaccine....
Porphyromonas gingivalis (Pg) is a keystone pathogen in periodontitis, highly prevalent disease manifested by chronic inflammation of the periodontium, alveolar bone resorption and tooth loss. During periodontitis pathobionts such as Pg can enter bloodstream growing evidence correlates with increased risk cardiovascular neurodegenerative diseases. However, mechanism which immune cells respond to challenge vivo remains elusive. produce aggressive proteolytic virulence factors termed...
In mammals, the relationship between immune system and behavior is widely studied. fish, however, knowledge concerning brain response behavioral changes during viral infection very limited. To further investigate this subject, we used model of tilapia lake virus (TiLV) zebrafish ( Danio rerio ), which was previously developed in our laboratory. We demonstrated that TiLV persists adult for at least 90 days, even when not detectable other peripheral organs. The virions were found whole brain....
Abstract Cyprinid herpesvirus 3 (CyHV-3), a member of the family Alloherpesviridae , is causative agent lethal disease in common and koi carp. CyHV-3 ORF134 encodes an interleukin-10 (IL-10) homologue. The present study was devoted to this ORF. Transcriptomic analyses revealed that expressed as spliced gene belonging early-late class. Proteomic infected cell supernatant demonstrated expression product one most abundant proteins secretome. To investigate role viral replication vitro virulence...
Cyprinid herpesvirus 3 (CyHV-3) infection in common carp Cyprinus carpio L. and its ornamental koi varieties can induce the severe systemic disease known as disease. This is characterised by a rapid replication spreading of virus through multiple organs results fast onset mortality (starting on Day 6 post infection) up to 100% infected fish. During first phase viral infections, type I interferons (IFNs) have generally been proven be essential inducing an innate immune response; however, very...
Stress may pose a serious challenge to immune homeostasis. however also prepare the system for challenges such as wounding or infection, which are likely happen during fight flight stress response. In common carp (Cyprinus carpio L.) we studied stress-induced redistribution of neutrophils into circulation, and expression genes encoding CXC chemokines known be involved in regulation neutrophil retention (CXCL12) (CXCL8), their receptors (CXCR4 CXCR1-2, respectively) blood leukocytes fish...
In vertebrate species, the innate immune system down-regulates protein translation in response to viral infection through action of double-stranded RNA (dsRNA)-activated kinase (PKR). some teleost species another kinase, Z-DNA-dependent (PKZ), plays a similar role but instead dsRNA binding domains, PKZ has Zα domains. These domains recognize left-handed conformer dsDNA and known as Z-DNA/Z-RNA. Cyprinid herpesvirus 3 infects common koi carp, which have PKZ, encodes ORF112 that itself bears...
Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of a lethal disease carp and encodes for an Il10 homolog (ORF134). Our previous studies with recombinant ORF134-deleted strain derived revertant suggested that cyprinid (CyHV-3 [cyhv3Il10]) not essential viral replication in vitro, or virulence vivo. In apparent contrast, cyhv3Il10 one most abundant proteins CyHV-3 secretome structurally very similar to also human IL10. To date, addressing biological activity on cells its natural host...
Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a highly virulent and lethal disease common carp Cyprinus carpio its ornamental koi varieties. However, specific knowledge about immune mechanisms behind infection process very limited. We aimed to evaluate effect CyHV-3 on profile 2 major components acute phase response: C-reactive protein (CRP) complement system. Common were infected with by bath immersion. Fish sampled before at 6, 12, 24, 72, 120 336 h post-infection for serum...