A5017641214- Aquaculture disease management and microbiota
- 3D Printing in Biomedical Research
- Zebrafish Biomedical Research Applications
- Orthopaedic implants and arthroplasty
- Cellular Mechanics and Interactions
- Microbial infections and disease research
- Bone Tissue Engineering Materials
- Immune cells in cancer
- Myxozoan Parasites in Aquatic Species
- Immune Cell Function and Interaction
- Physiological and biochemical adaptations
- Rabies epidemiology and control
- Viral Infections and Outbreaks Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Toxoplasma gondii Research Studies
- Invertebrate Immune Response Mechanisms
- Advanced Materials and Mechanics
- Aquaculture Nutrition and Growth
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Dental Implant Techniques and Outcomes
- Child Nutrition and Water Access
- Reproductive System and Pregnancy
- Additive Manufacturing and 3D Printing Technologies
- Viral Infections and Vectors
Eindhoven University of Technology
2022-2025
Wageningen University & Research
2015-2020
Bipar
2020
Macrophages play important roles in conditions ranging from host immune defense to tissue regeneration and polarize their functional phenotype accordingly. Next differences the use of L-arginine production different cytokines, inflammatory M1 macrophages anti-inflammatory M2 are also metabolically distinct. In mammals, show metabolic reprogramming towards glycolysis while rely on oxidative phosphorylation generate energy. The presence polarized phenotypes conserved mammals fish led us...
Abstract Mammalian macrophages can adopt polarization states that, depending on the exact stimuli present in their extracellular environment, lead to very different functions. Although these have been shown primarily for of humans and mice, it is likely that polarized with corresponding phenotypes exist across mammals. Evidence functional conservation from teleost fish suggests same, or at least comparable should also be teleosts. However, transcriptional profiles marker genes not reported...
Abstract Current challenges in tissue engineering include creation of extracellular environments that support and interact with cells using biochemical, mechanical, structural cues. Spatial control over these cues is currently limited due to a lack suitable fabrication techniques. This study introduces Xolography, an emerging dual‐color light‐sheet volumetric printing technology, achieve mechanical features for hydrogel‐based photoresins at micro‐ macroscale while within minutes. A...
Sphaerospora molnari is a myxozoan parasite causing skin and gill sphaerosporosis in common carp (Cyprinus carpio) central Europe. For most myxozoans, little known about the early development expansion of infection fish host, prior to spore formation. A major reason for this lack information absence laboratory model organisms, whose life-cycle stages are available throughout year.We have established proliferative based on separation intraperitoneal injection motile dividing S. isolated from...
Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of a lethal disease carp and encodes for an Il10 homolog (ORF134). Our previous studies with recombinant ORF134-deleted strain derived revertant suggested that cyprinid (CyHV-3 [cyhv3Il10]) not essential viral replication in vitro, or virulence vivo. In apparent contrast, cyhv3Il10 one most abundant proteins CyHV-3 secretome structurally very similar to also human IL10. To date, addressing biological activity on cells its natural host...
We recently reported on the functional characterization of carp Il10. showed that Il10 is able to downregulate proinflammatory activities by phagocytes and promote B cell proliferation, differentiation antibody production as well proliferation memory T cells. Taking advantage recent annotation genome, we completed sequence a second il10 paralogue, named il10b, presence which was expected owing (8 million years ago) fourth round whole genome duplication occurred in common carp. In present...
Mammalian granulocyte colony-stimulating factor (G-CSF; CSF3) is a primary cytokine that promotes the development, mobilization, and activation of neutrophils their precursors. Teleosts have been reported to possess two paralogs as likely result teleost-wide whole genome duplication (WGD) event, but functional divergence G-CSF remains poorly understood. Common carp are an allotetraploid species owing additional WGD event in lineage here, we report on genomic synteny, sequence similarity,...
Macrophages are amongst the first immune cells that encounter rabies virus (RABV) at entry sites. Activation of macrophages is essential for onset a potent response, but insights into effects RABV on macrophage activation scarce. In this study we performed high-throughput sequencing RNA extracted from were exposed to 48 hours, and compared their transcriptional profiles non-polarized (M0), polarized towards canonical M1, M2a M2c phenotypes. Our analysis revealed RABV-stimulated show high...
Human in vitro bone remodeling models, using osteoclast-osteoblast cocultures, can facilitate the investigation of human while reducing need for animal experiments. Although current cocultures have improved understanding remodeling, it is still unknown which culture conditions support both cell types. Therefore, models benefit from a thorough evaluation impact variables on turnover outcomes, with aim to reach balanced osteoclast and osteoblast activity, mimicking healthy remodeling. Using...
Abstract Current challenges in tissue engineering include creation of extracellular environments that support and interact with cells using biochemical, mechanical, structural cues. Spatial control over these cues is currently limited due to a lack suitable fabrication techniques. This study introduces Xolography, an emerging dual-color light-sheet volumetric printing technology, achieve mechanical features for hydrogel-based photoresins at micro-to macroscale while within minutes. We...
Abstract Human in vitro bone remodeling models, using osteoclast-osteoblast co-cultures, could facilitate the investigation of human healthy ( i . e ., balanced) and pathological unbalanced) while reducing need for animal experiments. Although current co-cultures have improved our understanding remodeling, they lack culture method outcome measurement standardization, which is hampering reproducibility translatability. Therefore, models benefit from a thorough evaluation impact variables on...