A5054667348- Adenosine and Purinergic Signaling
- Plant Gene Expression Analysis
- Biochemical and Molecular Research
- Autophagy in Disease and Therapy
- GABA and Rice Research
- Botanical Research and Chemistry
- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Sirtuins and Resveratrol in Medicine
- Plant Stress Responses and Tolerance
- Biochemical and biochemical processes
- Plant responses to water stress
- Genomics, phytochemicals, and oxidative stress
- Soybean genetics and cultivation
- Photosynthetic Processes and Mechanisms
- Calcium signaling and nucleotide metabolism
- Histone Deacetylase Inhibitors Research
- Plant Parasitism and Resistance
- Plant and fungal interactions
- Health, Environment, Cognitive Aging
- Biochemical Acid Research Studies
- Plant Genetic and Mutation Studies
- Light effects on plants
- Protein Tyrosine Phosphatases
- Piperaceae Chemical and Biological Studies
University of Life Sciences in Poznań
2011-2023
Universidad de Murcia
2014
University of Life Sciences in Lublin
2011-2013
University of Agriculture in Krakow
1999-2006
University of Agriculture Faisalabad
2005
Instytut Środowiska Rolniczego i Leśnego Polskiej Akademii Nauk
2003
Responses of barley seedlings to water deficit (WD) induced by polyethylene glycol (PEG 6000) and ultraviolet (UV-B; 280–320 nm) radiation their interaction (UV-B + WD) were examined. A decrease in dry matter yield content leaves roots was observed following application WD UV-B WD, while no changes found after treating plants with UV-B. Proline increased under conditions WD. In contrast, treatment had effect on the accumulation proline plants. Changes root showed a varied response: an...
It is known that cells under stress accumulate various dinucleoside polyphosphates, compounds suggested to function as alarmones. In plants, the phenylpropanoid pathways yield metabolites protecting these organisms against types of stress. Observations reported in this communication link two phenomena and provide an example a metabolic “addressee” for “alarm” signaled by diadenosine triphosphate (Ap 3 A) or tetraphosphate 4 A). response added Ap A A, seedlings Arabidopsis thaliana incubated...
Transglutaminases (E.C. 2.3.2.13) catalyze the post-translational modification of proteins by establishing ε-(γ-glutamyl) lysine isopeptide bonds and covalent conjugation polyamines to endo-glutamyl residues proteins. In light confirmed role transglutaminases in animal cell apoptosis only limited information on these enzymes plant senescence, we decided investigate activity chloroplast (ChlTGases) fate chloroplast-associated Hordeum vulgare L. 'Nagrad' leaves, where senescence process was...
Abstract 4-Coumarate:coenzyme A ligase (4CL) is known to activate cinnamic acid derivatives their corresponding coenzyme esters. As a new type of 4CL-catalyzed reaction, we observed the synthesis various mono- and diadenosine polyphosphates. Both native 4CL2 isoform from Arabidopsis (At4CL2 wild type) At4CL2 gain function mutant M293P/K320L, which exhibits capacity use broader range phenolic substrates, catalyzed adenosine 5′-tetraphosphate (p4A) 5′-pentaphosphate when incubated with MgATP−2...
We show here that Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly adenylylsulfatases and nucleoside phosphoramidases, liberating 5'-monophosphates from such natural metabolites adenosine 5'-phosphosulfate 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, 5'-O-phosphorofluoridate 5'-O-(gamma-fluorotriphosphate), release AMP them. With respect the former, behave like a phosphodiesterase I concomitant with cleavage of P-F...
Under nutrient deficiency or starvation conditions, the mobilization of storage compounds during seed germination is enhanced to primarily supply respiratory substrates and hence increase potential cell survival. Nevertheless, we found that, under sugar conditions in isolated embryonic axes white lupin (Lupinus albus L.) Andean mutabilis Sweet) cultured vitro for 96 h, disruption lipid breakdown occurs, as was reflected higher content sugar-starved (-S) than sucrose-fed (+S) axes. We...
The research was conducted on embryo axes of yellow lupin ( Lupinus luteus L.), white albus L.) and Andean mutabilis Sweet), which were isolated from imbibed seeds cultured for 96 h in vitro under different conditions carbon nitrogen nutrition. Isolated fed with 60 mM sucrose or sugar-starved. effect 35 asparagine (a central amino acid the metabolism germinating seeds) nitrate (used as an inorganic kind nitrogen) growth, storage lipid breakdown autophagy investigated. sugar-starved contained...
Adenosine 5′‐polyphosphates have been identified in vitro , as products of certain enzymatic reactions, and vivo . Although the biological role these compounds is not known, there exist highly specific hydrolases that degrade nucleoside into corresponding 5′‐triphosphates. One approach to understanding mechanism function enzymes through use specifically designed phosphonate analogues. We synthesized novel nucleotides: α,β‐methylene‐adenosine 5′‐tetraphosphate (pppCH 2 pA),...
A new methodology for synthesis of biologically important nucleoside tri- and tetraphosphates containing a bisphosphonate moiety instead the terminal pyrophosphate bond is described. The series consists tetraphosphate analogs adenosine, guanosine 7-methylguanosine (characteristic mRNA cap). We have adopted two-step procedure that allowed us to insert methylene bridge into phosphate chain. Nucleoside mono- or diphosphates were first activated (as imidazole derivatives) then used in coupling...
Dinucleoside polyphosphates (Np(n)N's; where N and N' are nucleosides n = 3-6 phosphate residues) naturally occurring compounds that may act as signaling molecules. One of the most successful approaches to understand their biological functions has been through use Np(n)N' analogs. Here, we present results studies using novel diadenosine polyphosphate analogs, with an oxymethylene group replacing one or two bridging oxygen(s) in chain. These have tested potential substrates and/or inhibitors...
Autophagy is a fundamental process for plants that plays crucial role in maintaining cellular homeostasis and promoting survival response to various environmental stresses. One of the lesser-known stages plant autophagy degradation autophagic bodies vacuoles. To this day, no vacuolar enzyme has been confirmed be involved process. On other hand, several enzymes have described yeast (Saccharomyces cerevisiae), including Atg15, possess lipolytic activity. In preliminary study, which was...