A5051158502- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- Hepatitis C virus research
- Liver Disease and Transplantation
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Liver Diseases and Immunity
- Virology and Viral Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Bacillus and Francisella bacterial research
- Animal Disease Management and Epidemiology
- Toxin Mechanisms and Immunotoxins
- interferon and immune responses
- Interprofessional Education and Collaboration
- Macrophage Migration Inhibitory Factor
- Drug-Induced Adverse Reactions
- Pharmacy and Medical Practices
- Plant Virus Research Studies
University of North Carolina at Chapel Hill
2020-2023
National Institute of Infectious Diseases
2009-2022
Foundation for Biomedical Research and Innovation
2022
Hyogo Brain and Heart Center
2017
The University of Tokyo
2007-2009
National Institutes of Health
2008
Monkeypox virus is divided into Congo Basin and West African strains. The virulence pathophysiology of two strains, Zr-599 (a monkeypox virus) Liberia virus), were evaluated in non-human primates. Four monkeys infected by the subcutaneous (SC) intranasal (IN) inoculation routes for at a dose 10(6) p.f.u. One monkey Liberia/SC group was demonstrated to be co-infected with Gram-positive cocci excluded from analyses. Infections three four Zr-599/SC one fatal. Virus genome levels blood...
Monkeypox virus (MPXV) causes a smallpox-like disease in non-human primates and humans. This infection is endemic to central western Africa. MPXV divided into two genetically different groups, Congo Basin West African MPXV, with the former being more virulent. A real-time quantitative genome amplification system was developed for diagnosis of infections using loop-mediated isothermal (LAMP) technology. Primers used (C-LAMP), (W-LAMP), both (COM-LAMP) by LAMP were designed according...
Although the C-terminal 52 amino acids (C52aa) of hepatitis E virus (HEV) capsid are not essential for morphology, C52aa-encoding region is required replication. Transfection a C52aa knockdown mutant showed transient growth, and earliest population included majority noninfectious (possibly empty) particles minority infectious with degradation. Finally, complete revertant was generated reproducibly. viral life cycle, promoting accurate encapsidation stabilizing encapsidated particles.
Despite excellent vaccines, resurgent outbreaks of hepatitis A have caused thousands hospitalizations and hundreds deaths within the United States in recent years. There is no effective antiviral therapy for A, many aspects virus (HAV) replication cycle remain to be elucidated. Replication requires zinc finger protein ZCCHC14 noncanonical TENT4 poly(A) polymerases with which it associates, but underlying mechanism unknown. Here, we show that TENT4A/B are required viral RNA synthesis...
Norovirus, which belongs to the family Caliciviridae, is one of major causes nonbacterial acute gastroenteritis in world. The main human noroviruses are genogroup I (GI) and II (GII), were subdivided further into at least 15 18 genotypes (GI/1 GI/15 GII/1 GII/18), respectively. development immunological diagnosis for norovirus had been hindered by antigen specificity polyclonal antibody. Therefore, several laboratories have produced broadly reactive monoclonal antibodies, recognize linear GI...
Hepatitis E virus (HEV) is a causative agent of acute hepatitis throughout the world. HEV genotypes 1 through 4 infect humans, whereas 3 and (Gt3 Gt4) also other animals. In developed countries, main infection route by foodborne transmission, resulting from consumption undercooked meat. It important to know criteria for control in daily cooking. this study, we assessed heat conditions required inactivate Gt3 Gt4 culture supernatants spiked minced pork inactivation was determined measuring...
Hepatitis E virus (HEV) causes acute and fulminant hepatitis worldwide. Although enveloped (e) non-enveloped (ne) forms of HEV have been discovered, host factors involved in infection, including receptors, remain to be elucidated. Here, we identified integrin α3 (encoded by ITGA3), a protein that binds responds the extracellular matrix, as an essential factor for infection. Integrin expression was lower four HEV-non-permissive cell subclones than HEV-permissive subclone. ITGA3 knockout cells...
PLC/PRF/5 cells show limited permissiveness, meaning that almost all subclones are permissive; however, some do not exhibit permissiveness for hepatitis E virus (HEV) infection. In this study, the single-cell cloning of was performed and heterogeneous characterized. Notably, efficiency intracellular replication did correlate with HEV However, as well binding permissive subclones, virus-like particles bound non-permissive on various levels, suggesting these have deficiencies in attachment...
Virus-induced changes in host lipid metabolism are an important but poorly understood aspect of viral pathogenesis. By combining nontargeted lipidomics analyses infected cells and purified extracellular quasi-enveloped virions with high-throughput RNA sequencing genetic depletion studies, we show that hepatitis A virus, hepatotropic picornavirus, broadly manipulates the cell environment, enhancing synthesis ceramides other sphingolipids transcriptionally activating acyl–coenzyme synthetases...
To understand the hepatitis E virus (HEV)-pollution status in environment Philippines, a total of 12 water samples were collected from rivers Manila City for detection HEV RNA. Three positive RNA indicating that is circulating Philippines. Phylogenetic analysis classified all sequences into genotype 3.
Abstract Norovirus (NoV) capsid proteins were expressed as virus‐like particles (VLPs) by using recombinant baculovirus in insect cells, which had 5 genotypes genogroup I and 11 II, the VLPs used immunogens. Polyclonal antibody against VLP of GII/3 genotype showed broad‐range cross‐reactivity, reacting not only with intra‐genogroup strains, but also inter‐genogroup antibody‐ELISA 16 kinds VLPs. Furthermore, antigen‐ELISA was conducted sandwich enzyme‐linked immunosorbent assay (ELISA)...
Mechanisms underlying the cellular release of quasi-enveloped hepatoviruses are only partially understood, yet play a crucial role in pathogenesis this common agent viral hepatitis. Multiple NEDD4 family E3 ubiquitin ligases, including ITCH, have been reported to promote budding conventional enveloped viruses but not known function HAV or other picornaviruses from infected cells.
Iminosugar compounds are monosaccharide mimetics with broad but generally weak antiviral activities related to inhibition of enzymes involved in glycobiology. Miglustat (N-butyl-1-deoxynojirimycin), which is approved for the treatment lipid storage diseases humans, and UV-4 [N-(9-methoxynonyl)-1-deoxynojirimycin] inhibit replication hepatitis A virus (HAV) cell culture (50% inhibitory concentrations [IC50s] 32.13 μM 8.05 μM, respectively) by blocking synthesis gangliosides essential HAV...
Hepatitis A virus (HAV) is a common infectious etiology of acute hepatitis worldwide. The Philippines remains highly endemic for A, but there still lack information about HAV in the country. To evaluate contamination environmental water Philippines, we conducted detection and genetic analyses RNA samples from river water. Twelve were collected at 6 sampling sites 3 rivers Metro Manila, both dry wet seasons 2012 2013. was detected all season, one sample season. Phylogenetic analysis confirmed...
Hepatitis E virus (HEV) is the causative agent of viral hepatitis E. In Japan, HEV genotype 3 (G3) and G4 are predominantly detected, while G1, mainly imported from countries in continental Asia, rare. present study, we detected a G1 strain patient who visited Japan India. When PLC/PRF/5 cells (subclone 4-21) were inoculated with stool suspension this patient, accumulation RNA was observed spent culture medium, indicating that had been successfully isolated specimen. A nearly complete genome...
Although hepatitis A virus (HAV) is associated only with acute in humans, HAV RNA persists within the liver for months following resolution of inflammation and cessation fecal shedding chimpanzees murine models A. Here, we confirm striking differences kinetics clearance from versus serum feces infected Ifnar1-/- mice investigate nature viral persisting normalization alanine aminotransferase (ALT) levels. Fecal produced hepatocytes declined >3,000-fold between its peak at day 14 126, whereas...
Herpes simplex virus type 1 (HSV-1) infections in a child with congenital immunodeficiency syndrome were observed over 10-year period. The suffered from recurrent and severe HSV-1 mucocutaneous infections. He frequently acyclovir (ACV)-resistant (ACVr) infection the later phase of his life, especially after episode ACVr infection. Virological analyses on isolates recovered this patient revealed that all thymidine kinase (TK)-negative (TK-) due to single cytosine (C) deletion within 4-C...
Emerging viruses continue to surprise us, providing many newly recognized diseases study and try control.Early discovery of infectious is necessary prevent potential outbreaks.Virus has been aided by the development sequenceindependent methods for obtaining genomic data.The most prominent these include representational difference analysis (RDA) sequence-independent single primer amplification (SISPA) with several variations [1][2][3]5,7,[10][11].However, sensitivity RDA method inadequate...
Introduction: Monkeypox virus (MPXV), a member of the genus Orthopoxvirus, causes smallpox-like disease, human monkeypox. This infection is endemic to central and western Africa. A sporadic outbreak monkeypox occurred in USA 2003, indicating necessity development diagnostics for even countries free from this infection. MPXV divided into two genetically different groups, Congo Basin West African groups. real-time quantitative genome amplification system, which differentiates was developed...