A5004838638- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Drug Transport and Resistance Mechanisms
- Cancer Research and Treatments
- Protein Interaction Studies and Fluorescence Analysis
- Analytical Chemistry and Sensors
- Alzheimer's disease research and treatments
- Trace Elements in Health
- Microbial Natural Products and Biosynthesis
- Biotin and Related Studies
- Chemical Synthesis and Analysis
- Enzyme Catalysis and Immobilization
- Click Chemistry and Applications
- Lipid Membrane Structure and Behavior
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
ETH Zurich
2023-2024
Max Planck Institute for Medical Research
2020-2024
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2022-2024
Vrije Universiteit Amsterdam
2023
Heidelberg University
2022
Heidelberg (Poland)
2020
Rhodamine fluorophores are setting benchmarks in fluorescence microscopy. Herein, we report the deuterium (d12) congeners of tetramethyl(silicon)rhodamine, obtained by isotopic labelling four methyl groups, show improved photophysical parameters (
Impermeable SNAP-tag substrates allow exclusive labelling of receptors on the cell membrane for nanoscopy, SiMPull and <italic>in vivo</italic> use.
Proteins are essential biomolecules and central to biotechnological applications. In many cases, assembly into higher-order structures is a prerequisite for protein function. Under conditions relevant applications, integrity often challenged, resulting in disassembly, aggregation, loss of The stabilization quaternary structure has proven challenging, particularly trimeric complexes, given the complexity involved inter- intramolecular interaction networks. Here, we describe chemical...
The prevailing but not undisputed amyloid cascade hypothesis places the β-site of APP cleaving enzyme 1 (BACE1) center stage in Alzheimer′s Disease pathogenesis. Here, we investigated functional properties BACE1 with novel tag- and antibody-free labeling tools, which are conjugates BACE1-inhibitor IV (also referred to as C3) linked different impermeable Alexa Fluor dyes. We show that these fluorescent small molecules bind specifically BACE1, a 1:1 stoichiometry at their orthosteric site....
We describe a biosensor for proteolytic cleavage combining tailored fluidic chips with pulsed-field magnetometer integrated into printed circuit boards.
Abstract Bacteria‐based agents are emerging as promising tools for cancer therapy due to their ability actively target tumors, trigger localized inflammation, and induce tumor regression. There has been growing interest in using bacteria that responsive external cues, such magnetic fields, facilitate the formation of robust colonies tumors achieve threshold clinical efficacy. Several studies have demonstrated potential innately magnetically bacteria, known magnetotactic (MTB), steerable...
Abstract Employing self-labelling protein tags for the attachment of fluorescent dyes has become a routine and powerful technique in optical microscopy to visualize track fused proteins. However, membrane permeability associated background signals can interfere with analysis extracellular labeling sites. Here we describe novel approach improve by functionalizing SNAP-tag substrate benzyl guanine (“BG”) charged sulfonate (“SBG”). This chemical manipulation improves solubility, reduces...