A5015556753- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Memory and Neural Mechanisms
- Light effects on plants
- Neural dynamics and brain function
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac electrophysiology and arrhythmias
- Supramolecular Self-Assembly in Materials
- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- Chemical Synthesis and Analysis
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- Axon Guidance and Neuronal Signaling
- Advanced Fluorescence Microscopy Techniques
- Neurogenesis and neuroplasticity mechanisms
- Treatment of Major Depression
- Nicotinic Acetylcholine Receptors Study
- Plant Molecular Biology Research
- Plant and animal studies
- Plant and Biological Electrophysiology Studies
- Neurotransmitter Receptor Influence on Behavior
University of California, Irvine
2016-2025
University of Vermont Medical Center
2022
Varian Medical Systems (United States)
2009-2021
University of California System
2017-2019
Massachusetts Institute of Technology
1993-2018
New York University
1998-2018
Columbia University
2001
Harvard University
2000
McLean Hospital
2000
Brandeis University
1996-1997
The task group (TG) for quality assurance of medical accelerators was constituted by the American Association Physicists in Medicine's Science Council under direction Radiation Therapy Committee and Quality Assurance Outcome Improvement Subcommittee. (TG‐142) had two main charges. First to update, as needed, recommendations Table II AAPM TG‐40 report on second, add asymmetric jaws, multileaf collimation (MLC), dynamic/virtual wedges. TG accomplished update TG‐40, specifying new test...
A 16-residue peptide [(Ala-Glu-Ala-Glu-Ala-Lys-Ala-Lys)2] has a characteristic beta-sheet circular dichroism spectrum in water. Upon the addition of salt, spontaneously assembles to form macroscopic membrane. The membrane does not dissolve heat or acidic alkaline solutions, nor it upon guanidine hydrochloride, SDS/urea, variety proteolytic enzymes. Scanning EM reveals network interwoven filaments approximately 10-20 nm diameter. An important component stability is probably due formation...
A new type of s elf- a ssembling peptide (sapeptide) scaffolds that serve as substrates for neurite outgrowth and synapse formation is described. These peptide-based are amenable to molecular design by using chemical or biotechnological syntheses. They can be tailored variety applications. The sapeptide formed through the spontaneous assembly ionic self-complementary β-sheet oligopeptides under physiological conditions, producing hydrogel material. support neuronal cell attachment...
The charges on this task group (TG) were as follows: (a) provide specific procedural guidelines for performing the tests recommended in TG 142; (b) estimate of range time, appropriate personnel, and qualifications necessary to complete (c) sample daily, weekly, monthly, or annual quality assurance (QA) forms. Many report are drawn from literature included references. When was not available, test methods reflect experiences members (e.g., a method door interlock is self-evident with no...
Coupling of autonomous cellular oscillators is an essential aspect circadian clock function but little known about its circuit requirements. Functional ablation the pigment-dispersing factor-expressing lateral ventral subset (LN V ) Drosophila neurons abolishes rhythms locomotor activity. The hypothesis that LN s synchronize oscillations in downstream was tested by rendering hyperexcitable via transgenic expression a low activation threshold voltage-gated sodium channel. When are made...
The human Kv1.5 potassium channel (hKv1.5) contains proline-rich sequences identical to those that bind Src homology 3 (SH3) domains. Direct association of the tyrosine kinase with cloned hKv1.5 and native in myocardium was observed. This interaction mediated by motif SH3 domain Src. Furthermore, phosphorylated, current suppressed, cells coexpressing v-Src. These results provide direct biochemical evidence for a signaling complex composed protein kinase.
Light-responsive neural activity in central brain neurons is generally conveyed through opsin-based signaling from external photoreceptors. Large lateral ventral arousal (lLNvs) Drosophila melanogaster increase action potential firing within seconds response to light the absence of all Light-evoked changes membrane resting occur about 100 milliseconds. The selective for blue wavelengths corresponding spectral sensitivity CRYPTOCHROME (CRY). cry-null lines are light-unresponsive, but restored...
A subset of Drosophila neurons that expresses crustacean cardioactive peptide (CCAP) has been shown previously to make the hormone bursicon, which is required for cuticle tanning and wing expansion after eclosion. Here we present evidence CCAP-expressing (NCCAP) consist two functionally distinct groups, one releases bursicon into hemolymph other regulates its release. The first group, call NCCAP-c929, includes 14 bursicon-expressing abdominal ganglion lie within expression pattern...
The ventral lateral neurons (LNvs) of adult Drosophila brain express oscillating clock proteins and regulate circadian behavior. Whole cell current-clamp recordings large LNvs in freshly dissected whole preparations reveal two spontaneous activity patterns that correlate with underlying membrane potential: tonic burst firing sodium-dependent action potentials. Resting potential are rapidly reversibly regulated by acute changes light intensity. LNv electrophysiological response is attenuated,...
Circadian clocks regulate much of behavior and physiology, but the mechanisms by which they do so remain poorly understood. While cyclic gene expression is thought to underlie metabolic rhythms, little known about cycles in cellular physiology. We found that Drosophila insulin-producing cells (IPCs), are located pars intercerebralis lack an autonomous circadian clock, functionally connected central clock circuit via DN1 neurons. Insulin mediates output regulating rhythmic a (sxe2) fat body....
Microglia have crucial roles in sculpting synapses and maintaining neural circuits during development. To test the hypothesis that microglia continue to regulate circuit connectivity adult brain, we investigated effects of chronic microglial depletion, via CSF1R inhibition, on synaptic visual cortex mice both sexes. We find absence dramatically increases excitatory inhibitory connections cortical neurons assessed with functional mapping experiments acutely prepared brain slices. depletion...
Significance CRYPTOCHROMES (CRYs) are blue light photoreceptors that mediate phototransduction in brain arousal neurons, as well circadian entrainment Drosophila fruit flies. We describe how light-activated CRY couples to membrane depolarization and increased action potential firing rate large ventral lateral neurons. Pharmacological treatments specifically disrupt the redox-sensitive flavin chromophore or block voltage-gated K + channels abolish response. Correspondingly, we find Kvβ...
Psychedelic drugs have reemerged as tools to treat several brain disorders. Cultural attitudes toward them are changing, and scientists once again investigating the neural mechanisms through which these impact function. The significance of this research direction is reflected by recent work, including work presented authors at 2022 meeting Society for Neuroscience. As 2022, there were hundreds clinical trials recruiting participants testing therapeutic effects psychedelics. Emerging evidence...
Kv1.3, a voltage-dependent potassium channel cloned from mammalian brain and T lymphocytes, contains multiple tyrosine residues that are putative targets for kinases. We have examined the phosphorylation of expressed transiently in human embryonic kidney (or HEK) 293 cells, by endogenous coexpressed Tyrosine is measured strategy immunoprecipitation followed by. Western blot analysis, using antibodies specifically recognize Kv1.3 phosphotyrosine. Coexpression constitutively active kinase...
The ionotropic glutamate receptor (iGluR) gene family has been widely studied in animals and is determined to be important excitatory neurotransmission other neuronal processes. We have previously identified receptor–like genes (GLRs) Arabidopsis thaliana, an organism that lacks a nervous system. Upon the completion of genome sequencing project, large GLR uncovered. A preliminary phylogenetic analysis divides AtGLR into three clades used as basis for recently established nomenclature family....
<i>Drosophila melanogaster</i> is a leading genetic model system in nervous development and disease research. Using the power of fly genetics traumatic axonal injury research will significantly speed up characterization molecular processes that control regeneration CNS. We developed versatile physiologically robust preparation for long-term culture whole <i>Drosophila</i> brain. use this method to develop novel CNS regeneration. first show that, similar mammalian axons, injured adult...