A5046222389- Muscle Physiology and Disorders
- Systemic Lupus Erythematosus Research
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Advanced Sensor and Energy Harvesting Materials
- ATP Synthase and ATPases Research
- Connective tissue disorders research
- Metabolism and Genetic Disorders
- Proteoglycans and glycosaminoglycans research
- Coenzyme Q10 studies and effects
- Cardiomyopathy and Myosin Studies
- Pharmacology and Obesity Treatment
- Biochemical Acid Research Studies
- Corneal Surgery and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Signaling Pathways in Disease
- Muscle activation and electromyography studies
- Therapeutic Uses of Natural Elements
- Clusterin in disease pathology
- Shoulder Injury and Treatment
- Sirtuins and Resveratrol in Medicine
- Exercise and Physiological Responses
University of California, Los Angeles
2019-2024
University of California, Irvine
2011-2015
BioClinica (United States)
2012-2014
Oklahoma Medical Research Foundation
2005
Abstract The maintenance of cellular function relies on the close regulation adenosine triphosphate (ATP) synthesis and hydrolysis. ATP hydrolysis by mitochondrial Synthase (CV) is induced loss proton motive force inhibited protein ATPase inhibitor (ATPIF1). extent CV hydrolytic activity its impact energetics remains unknown due to lack selective inhibitors CV. We find that takes place in coupled intact mitochondria increased respiratory chain defects. identified (+)‐Epicatechin as a binds...
Abstract Urodele amphibians are unique among adult vertebrates in their ability to regenerate complex body structures after traumatic injury. In salamander regeneration, the cells maintain a memory of original position and use this positional information recreate missing pattern. We used an vivo gain‐of‐function assay determine whether components extracellular matrix (ECM) have required induce formation new limb pattern during regeneration. discovered that ECM has position‐specific either...
The present single-dose study was performed to assess the effect of whole coffee fruit concentrate powder (WCFC), green caffeine (N677), grape seed extract (N31) and bean (N625) on blood levels brain-derived neurotrophic factor (BDNF). Randomly assorted groups fasted subjects consumed a single, 100 mg dose each material. Plasma samples were collected at time zero ( T 0 ) 30 min intervals afterwards, up 120 min. A total two control included: treated with silica dioxide (as placebo) or no...
Abstract Background The dystrophin-glycoprotein complex (DGC) is a critical adhesion of the muscle cell membrane, providing mechanical link between extracellular matrix (ECM) and cortical cytoskeleton that stabilizes sarcolemma during repeated contractions. One integral component DGC transmembrane protein, sarcospan (SSPN). Overexpression SSPN in skeletal mdx mice (murine model DMD) restores fiber attachment to ECM part through an associated increase utrophin integrin complexes at protecting...
Central for wound healing is the formation of granulation tissue, which largely consists collagen and whose importance stretches past healing, including being implicated in both fibrosis skin aging. Cyclophilin D (CyD) a mitochondrial protein that regulates permeability transition pore, known its role apoptosis ischemia-reperfusion. To date, CyD human generation has been unexplored. Here, we show was upregulated normal wounds venous ulcers, likely adaptive as inhibition impaired...
Knee osteoarthritis (OA) is a common degenerative joint disease which contributes significantly to the burden of physical disability.Conventional management OA mainly focuses on relief symptoms using analgesics and non-steroidal anti-inflammatory drugs (NSAIDs).Some dietary supplements have shown some potency reduce associated with conditions.Calcium Fructoborate (commercially marketed under trade name FruiteX-B®), naturally occurring borate complex as first described by Patrick Brown...
Aim: We aimed to evaluate the effect of a betalain-rich red beet concentrate (BRC) on joint discomfort and function. Methods: Individuals with self-reported knee were randomized blinded treatment either oral BRC (50 mg twice day) or placebo. Symptoms function assessed using Western Ontario McMaster Universities Arthritis Index (WOMAC) McGill Pain Questionnaire (MPQ) before after 5 10 days treatment. The general level energy was evaluated visual analog scale (VAS) at same time points....
The role of somatostatin (SRIF) on adenohypophysial hormone secretion in starved rats was reassessed by passive immunization. Because the absence pulsatile GH rats, effects injection SRIF antiserum levels can be clearly demonstrated. To determine whether starvation modifies sensitivity adenohypophysis to SRIF, we measured 125I-labelled iodo-N-Tyr-SRIF binding. There no difference dissociation constant (Kd) nor maximal binding capacity (Bmax) fed (n = 15) and animals (Kd 0.38 +/- 0.09...
Abstract: Calcium fructoborate (CFB) at a dose of 110 mg twice per day was previously reported to improve knee discomfort during the first 14 days treatment. In this study, 60 participants with self-reported were randomized into two groups receiving CFB or placebo. Initial levels evaluated by Western Ontario and McMaster Universities Arthritis Index (WOMAC) McGill Pain Questionnaire (MPQ) scores beginning study also 7 after Results showed that supplementation significantly improved in...
Mitochondrial depolarization can initiate reversal activity of ATP synthase, depleting by its hydrolysis. We have recently shown that increased hydrolysis contributes to depletion leading a maladaptation in mitochondrial disorders, where maximal hydrolytic capacity per CV content is increasing. However, despite importance, not commonly studied parameter because the limitations currently available methods. Methods measure indirectly require isolation mitochondria and involve introduction...
A pilot study by Reyes [1] previously showed that ingestion of single dose whole coffee fruit concentrate (WCFC) powder increased blood levels brain derived neurotrophic factor (BDNF) during the first 60 minutes after ingestion. In present report, we performed a dose, placebo-controlled, within-subject to confirm and further investigate this effect. Twenty healthy subjects with ages ranging from 25 35 participated in study. All fasted resting received placebo on Day 1, WCFC 2, cup freshly...
Abstract Background Duchenne muscular dystrophy (DMD) is caused by loss of sarcolemma connection to the extracellular matrix. Transgenic overexpression transmembrane protein sarcospan (SSPN) in DMD mdx mouse model significantly reduces disease pathology restoring membrane adhesion. Identifying SSPN-based therapies has potential benefit patients with and other forms dystrophies deficits muscle cell Methods Standard cloning methods were used generate C2C12 myoblasts stably transfected a...
Abstract Background Duchenne muscular dystrophy (DMD) is a degenerative muscle disease caused by mutations in the dystrophin gene. Loss of prevents formation critical connection between cell membrane and extracellular matrix. Overexpression sarcospan (SSPN) mouse model DMD restores reduces severity, making SSPN promising therapeutic target for pharmacological upregulation. Methods Using previously described cell-based promoter reporter assay gene expression (hSSPN-EGFP), we conducted...
Many people perceive bitterness when consuming broccoli. Glucosinolates and their degraded products are the main contributors to bitter taste. Production hydrolysis of glucosinolates require c gene using CRISPR-Cas9 will reduce broccoli’s This study also hypothesized that knocking out myrosinase encoding in The research involves designing a plasmid deliver system target disrupt enzyme gene. Successful completion this proposal could lead development broccoli variety is more palatable broader...
Acute clinical testing was performed on healthy human subjects to verify whether a single 150mg dose of proprietary formula marketed under the trade name "Mitochroma TM " (MCRC) can actually increase blood levels ATP.Sera and were collected immediately prior treatment at times 30, 60, 90 minutes after measure amounts ATP, lactate, ROS, pO 2 .Additionally, whole 270 expression selected cytokines chemokines.In comparison placebo group, samples from treated with MCRC showed increased total ATP...
Background: Dermaval™ is a composite formulation of various phytochemical-rich plant materials as quantified using high-performance liquid chromatography. This blend exerts inhibitory activity on human neutrophil elastase (HNE). An acute, crossover clinical study was performed to assess the effects Dermaval glucose-induced HNE in 20 healthy subjects. Methods: Participants served their own controls during this 3-day trial. On day 1, all participants were fasted and given only 300 mL water....
<h3>Purpose</h3> The Gullah population has a very low level of European admixture and higher homogeneity in terms environmental genetic influences. incidence systemic lupus erythematosus (SLE) is high the clinical manifestations SLE patients are also often severe. Therefore, such characteristics may help us identify more easily, through gene expression microarrays, genes involved pathogenesis SLE. We studied Epstein-Barr virus transformed B cell lines derived from matched unrelated controls...