A5009929479- Muscle Physiology and Disorders
- Protease and Inhibitor Mechanisms
- Blood properties and coagulation
- Genetic Neurodegenerative Diseases
- Tissue Engineering and Regenerative Medicine
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Muscle metabolism and nutrition
- Peptidase Inhibition and Analysis
- CRISPR and Genetic Engineering
- Advanced Proteomics Techniques and Applications
- Venous Thromboembolism Diagnosis and Management
- Drug-Induced Hepatotoxicity and Protection
- Erythropoietin and Anemia Treatment
- Electrospun Nanofibers in Biomedical Applications
- Sirtuins and Resveratrol in Medicine
- Acute Ischemic Stroke Management
- Nanoparticle-Based Drug Delivery
- Protein Interaction Studies and Fluorescence Analysis
- Peripheral Artery Disease Management
- Nanoparticles: synthesis and applications
- Plant Virus Research Studies
- Renin-Angiotensin System Studies
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Connective tissue disorders research
University of Colorado Denver
2019-2025
University of Colorado Anschutz Medical Campus
2020-2024
Santa Clara University
2023
Denver School of Nursing
2022
The extracellular matrix is a key component of tissues, yet it underrepresented in proteomic datasets. Identification and evaluation proteins the (ECM) has proved challenging due to insolubility many ECM traditional protein extraction buffers. Here we separate decellularization steps several prominent methods for under real-world conditions. results are used optimize two-fraction method. Approximately one dozen additional parameters tested, recommendations analysis based on overall coverage...
Engineered nanomaterials (ENMs) are synthesized with a diversity of surface chemistries that mediate biochemical interactions and physiological response to the particles. In this work, silver engineered (AgENMs) used evaluate role charge in protein cellular cytotoxicity. The most abundant blood, human serum albumin (HSA), was interacted 40 nm AgENMs range surface-charged coatings: positively charged branched polyethyleneimine (bPEI), negatively citrate (CIT), circumneutral poly(ethylene...
Abstract We developed an on-slide decellularization approach to generate acellular extracellular matrix (ECM) myoscaffolds that can be repopulated with various cell types interrogate cell-ECM interactions. Using this platform, we investigated whether fibrotic ECM scarring affected human skeletal muscle progenitor (SMPC) functions are essential for myoregeneration. SMPCs exhibited robust adhesion, motility, and differentiation on healthy muscle-derived myoscaffolds. All SPMC interactions from...
Abstract Background The dystrophin-glycoprotein complex (DGC) is a critical adhesion of the muscle cell membrane, providing mechanical link between extracellular matrix (ECM) and cortical cytoskeleton that stabilizes sarcolemma during repeated contractions. One integral component DGC transmembrane protein, sarcospan (SSPN). Overexpression SSPN in skeletal mdx mice (murine model DMD) restores fiber attachment to ECM part through an associated increase utrophin integrin complexes at protecting...
Pulmonary hypertension (PH) is a progressive vascular disease characterized by remodeling, stiffening, and luminal obstruction, driven dysregulated cell proliferation, inflammation, extracellular matrix (ECM) alterations. Despite the recognized contribution of ECM dysregulation to PH pathogenesis, precise molecular alterations in matrisome remain poorly understood. In this study, we employed matrisome-focused proteomics approach map protein composition young bovine calf model acute...
The roles of factor XIIIa-specific cross-links in thrombus formation, regression, or probability for embolization are largely unknown. A molecular understanding fibrin architecture at the level these could inform development therapeutic strategies to prevent sequelae thromboembolism. Here, we present an MS-based method map native XIIIa insoluble matrix component whole-blood plasma-fibrin clots and vivo thrombi. Using a chaotrope-insoluble digestion quantitative cross-linking MS, identified...
Abstract The assembly and function of the yeast general transcription factor TFIID complex requires specific contacts between its Taf14 Taf2 subunits, however, mechanism underlying these remains unclear. Here, we determined molecular structural basis by which YEATS ET domains bind to C-terminal tail identified a unique DNA-binding activity linker region connecting two domains. We show that in absence ligands is occluded surrounding domains, therefore DNA binding autoinhibited. Binding...
BACKGROUND Sex dimorphisms in coagulation are well established, with female-specific hypercoagulability conferring a survival benefit the setting of trauma-induced coagulopathy (TIC). The mechanism behind these phenomena remains to be elucidated. We hypothesize that estradiol provokes hypercoagulable profile and alters clot proteomics fibrin crosslinking. METHODS Whole blood was collected from healthy adult volunteers (n = 30). A battery thrombelastography (TEG) assays (native, kaolin,...
ABSTRACT We developed an on-slide decellularization approach to generate acellular extracellular matrix (ECM) scaffolds that can be repopulated with various cell types interrogate cell-ECM interactions. Using this platform, we investigated whether fibrotic ECM scarring affected human skeletal muscle progenitor (SMPC) functions are essential for myoregeneration. SMPCs exhibited robust adhesion, motility, and differentiation on healthy muscle-derived myoscaffolds. All SPMC interactions...
Abstract Collagen cross-links created by the lysyl oxidase and hydroxylase families of enzymes are a significant contributing factor to biomechanical strength rigidity tissues, which in turn influence cell signaling ultimately phenotype. In clinic, proteolytically liberated N-terminal cross-linked peptide collagen I (NTX) is used as biomarker bone connective tissue turnover, altered several disease processes. Despite clinical utility these breakdown products, majority species have not been...
ABSTRACT Organisms with smaller genomes often perform multiple functions using one multi-subunit protein complex. The S. cerevisiae Silent Information Regulator complex (SIRc) carries out all of the core heterochromatin. SIR complexes first drive initiation and spreading histone deacetylation in an iterative manner. Subsequently, same are incorporated stably nucleosomes, driving compaction repression underlying chromatin domain. These two distinct SIRc have each been characterized much...
ABSTRACT The extracellular matrix is a key component of tissues, yet it under-represented in proteomic datasets. Identification and evaluation proteins the (ECM) has proved challenging due to insolubility many ECM traditional protein extraction buffers. Here we separate decellularization steps several prominent methods for under real-world conditions. results are used optimize two-fraction method. Approximately one dozen additional parameters tested recommendations analysis based on overall...
ABSTRACT Background The dystrophin-glycoprotein complex (DGC) is a critical adhesion of the muscle cell membrane, providing mechanical link between extracellular matrix (ECM) and cortical cytoskeleton that stabilizes sarcolemma during repeated contractions. One integral component DGC transmembrane protein, sarcospan (SSPN). Overexpression SSPN in skeletal mdx mice (murine model DMD) restores fiber attachment to ECM part through an associated increase utrophin integrin complexes at protecting...
Objectives: The incidence of acute limb ischemia (ALI) has increased since the emergence coronavirus disease 2019 (COVID-19) and been linked to serum level cross-linked fibrin degradation products (D-dimer). purpose this study is understand arterial thromboembolic architecture in patients with ALI how altered by COVID-19 infection. Methods: Arterial specimens were prospectively collected at a single institution, stored an IRB-approved tissue bank -80°C for preservation architecture. Liquid...