A5061314967- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Advanced Fluorescence Microscopy Techniques
- Genetics and Neurodevelopmental Disorders
- Advanced Memory and Neural Computing
- Photoacoustic and Ultrasonic Imaging
- Visual perception and processing mechanisms
- Cell Image Analysis Techniques
- Memory and Neural Mechanisms
- Autism Spectrum Disorder Research
- Optical Coherence Tomography Applications
- Electrochemical sensors and biosensors
- EEG and Brain-Computer Interfaces
- Advanced Electron Microscopy Techniques and Applications
- Ion channel regulation and function
- Conducting polymers and applications
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Advanced Biosensing Techniques and Applications
- Hearing, Cochlea, Tinnitus, Genetics
- 3D Printing in Biomedical Research
- Advanced X-ray Imaging Techniques
Purdue University West Lafayette
2016-2025
State Street (United States)
2018
Howard Hughes Medical Institute
2003-2013
Massachusetts Institute of Technology
2009-2013
The University of Texas Southwestern Medical Center
2003-2007
Center for Neurosciences
2005
Synaptic cell adhesion is central for synapse formation and function. Recently, the synaptic molecules neuroligin 1 (NL1) SynCAM were shown to induce presynaptic differentiation in cocultured neurons when expressed a non-neuronal cell. However, it uncertain how similar resulting artificial synapses are regular synapses. Are these isofunctional, or do all neuronal nonspecifically activate formation? To address questions, we analyzed properties of induced by NL1 SynCAM, compared actions with...
To study synapse formation by neuroligins, we co-cultured hippocampal neurons with COS cells expressing wild type and mutant neuroligins. The large size of makes it possible to test the effect neuroligins presented over an extended surface area. We found that a uniform lawn displayed on cell triggers hundreds uniformly sized, individual synaptic contacts are labeled neurexin antibodies. Electron microscopy revealed these artificial synapses contain presynaptic active zone docked vesicles...
Visual systems have evolved to recognize and extract features from complex scenes using limited sensory information. Contour perception is essential this process can occur despite breaks in the continuity of neighboring features. Such robustness animal visual system degraded or occluded shapes may also give rise an interesting phenomenon optical illusions. These illusions provide a great opportunity decipher neural computations underlying contour integration object detection. Kanizsa...
The roles played by cortical inhibitory neurons in experience-dependent plasticity are not well understood. Here we evaluate the participation of parvalbumin-expressing (PV+) GABAergic two forms modification primary visual cortex (V1) adult mice: ocular dominance (OD) resulting from monocular deprivation and stimulus-selective response potentiation (SRP) enriched experience. These triggered different events but lead to a similar increase response. Both also require NMDA class glutamate...
Neuroligins, proteins of the α/β-hydrolase fold family, are found as postsynaptic transmembrane whose extracellular domain associates with presynaptic partners, neurexin family. To characterize molecular basis neuroligin interaction neurexin-β, we expressed five soluble and exportable forms neuroligin-1 from recombinant DNA sources, by truncating protein before span near its carboxyl terminus. The functional a dimer when analyzed sedimentation equilibrium. By surface plasmon resonance,...
Patch clamp is the main technique for measuring electrical properties of individual cells. Since its discovery in 1976 by Neher and Sakmann, patch has been instrumental broadening our understanding fundamental ion channels synapses neurons. The conventional patch-clamp method requires manual, precise positioning a glass micropipette against cell membrane visually identified target neuron. Subsequently, tight "gigaseal" connection between pipette established, suction applied to establish...
Abstract Functional interfaces between electronics and biological matter are essential to diverse fields including health sciences bio-engineering. Here, we report the discovery of spontaneous (no external energy input) hydrogen transfer from glucose reactions into SmNiO 3 , an archetypal perovskite quantum material. The enzymatic oxidation is monitored down ~5 × 10 −16 M concentration via nickelate lattice. atoms donate electrons Ni d orbital induce electron localization through strong...
Abstract Single-molecule super-resolution microscopy allows pin-pointing individual molecular positions in cells with nanometer precision. However, achieving resolution through tissues is often difficult because of optical scattering and aberrations. We introduced 4Pi single-molecule nanoscopy for brain in-situ point spread function retrieval opaque tissue (4Pi-BRAINSPOT), integrating switching dynamic coherent PSF modeling, compatible clearing, light-sheet illumination, a novel quantitative...
Abstract Potassium channels regulate membrane potential and diverse physiological processes, including cell migration. However, the specific function of inwardly rectifying potassium in immune chemotaxis is unknown. Here, we identified that channel Kir7.1 (KCNJ13) maintains resting required for directional sensing during neutrophil chemotaxis. Pharmacological or genetic inhibition neutrophils impaired direction toward various chemoattractants without affecting polarization multiple models....
Familiarity of the environment changes way we perceive and encode incoming information. However, neural substrates underlying this phenomenon are poorly understood. Here describe a new form experience-dependent low-frequency oscillations in primary visual cortex (V1) awake adult male mice. The emerged visually evoked potentials single-unit activity following repeated stimulation. were sensitive to spatial frequency content stimulus required mAChRs for their induction expression. Finally,...
Glutamate, one of the main neurotransmitters in brain, plays a critical role communication between neurons, neuronal development, and various neurological disorders. Extracellular measurement such as glutamate brain is important for understanding these processes developing new generation brain–machine interfaces. Here, we demonstrate use perovskite nickelate–Nafion heterostructure promising sensor with low detection limit 16 nM response time 1.2 s via amperometric sensing. We have designed...
Abstract The inhomogeneous refractive indices of biological tissues blur and distort single-molecule emission patterns generating image artifacts decreasing the achievable resolution localization microscopy (SMLM). Conventional sensorless adaptive optics methods rely on iterative mirror changes image-quality metrics. However, these metrics result in inconsistent metric responses thus fundamentally limit their efficacy for aberration correction tissues. To bypass trial-then-evaluate...
Fragile X syndrome (FX), the most common inherited form of autism and intellectual disability, is a condition associated with visual perceptual learning deficits. We recently discovered that experience can encode familiarity via persistent low-frequency oscillations in mouse primary cortex (V1). Here, we combine this paradigm multifaceted experimental approach to identify neurophysiological impairments these FX mice. Extracellular recordings reveal shorter durations, lower power, frequencies...
The roles of bioelectric signaling in developmental patterning remain largely unknown, although recent work has implicated signals cellular processes such as proliferation and migration. Here, we report a mutation the inwardly rectifying potassium channel (kir) gene, kcnj13/kir7.1, that causes elongation fins zebrafish insertional mutant Dhi2059. A viral DNA insertion into noncoding region kcnj13 results transient activation ectopic expression somite dermomyotome, from which fin ray...
Neural circuits underlying brain functions are vulnerable to damage, including ischemic injury, leading neuronal loss and gliosis. Recent technology of direct conversion endogenous astrocytes into neurons in situ can simultaneously replenish the population reverse glial scar. However, whether these newly reprogrammed undergo normal development, integrate existing circuit, acquire functional properties specific for this circuit is not known. We investigated effect NeuroD1-mediated vivo...
Neural oscillations play critical roles in information processing, communication between brain areas, learning, and memory. We have recently discovered that familiar visual stimuli can robustly induce 5-Hz the primary cortex (V1) of awake mice after experience. To gain more mechanistic insight into this phenomenon, we used vivo patch-clamp recordings to monitor subthreshold activity individual neurons during these oscillations. analyzed tuning properties V1 naive experienced assess effect...
Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well electrical activity excitable cells. Despite its usefulness decades development, amplifiers required for patch are expensive bulky. This limited scalability throughput single-ion single-cell analyses. In this work, we have developed custom patch-clamp amplifier microchip can be fabricated using standard commercial...
Visual perceptual experience induces persistent 4-8 Hz oscillations in the mouse primary visual cortex (V1), encoding familiarity. Recent studies suggest that higher-order areas (HVAs) are functionally specialized and segregated into information streams processing distinct features. However, whether memories processed stored within is not understood. We report here V1 lateromedial (LM), but anterolateral, become more phase synchronized after entrainment of stimulus maximally responses LM....