A5068988240- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Synthesis and Biological Evaluation
- DNA and Nucleic Acid Chemistry
- Toxin Mechanisms and Immunotoxins
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Cell death mechanisms and regulation
- Heat shock proteins research
- Insect Pest Control Strategies
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Synthesis and biological activity
- Ferrocene Chemistry and Applications
- Metal complexes synthesis and properties
- Genetic factors in colorectal cancer
- Parasites and Host Interactions
- Blood Coagulation and Thrombosis Mechanisms
- Colorectal Cancer Treatments and Studies
- Fungal and yeast genetics research
- Parasitic Infections and Diagnostics
- thermodynamics and calorimetric analyses
- RNA and protein synthesis mechanisms
- Enzyme function and inhibition
Universidad de Alcalá
2015-2024
Navarre Institute of Health Research
2019
Consejo Superior de Investigaciones Científicas
1988-2015
Hospital Universitario Príncipe de Asturias
1995-2014
Universidad de Guadalajara
2012
Hôtel-Dieu de Paris
2001
Centro de Biología Molecular Severo Ochoa
1988-1999
Universidad Autónoma de Madrid
1988-1998
University of California, Davis
1995
The execution of the apoptotic death program in metazoans is characterized by a sequence morphological and biochemical changes that include cell shrinkage, presentation phosphatidylserine at surface, mitochondrial alterations, chromatin condensation, nuclear fragmentation, membrane blebbing formation bodies. Methodologies for measuring apoptosis are based on these markers. Except bodies, all other events have been observed most protozoan parasites undergoing death. However, while techniques...
Apoptosis is a normal component of the development and health multicellular organisms. However, apoptosis now considered prerogative unicellular organisms, including trypanosomatids genera Trypanosoma spp. Leishmania spp., causative agents some most important neglected human diseases. Trypanosomatids show typical hallmarks apoptosis, although they lack key molecules contributing to this process in metazoans, like caspase genes, Bcl-2 family genes TNF-related receptors. Despite these...
ABSTRACT A series of new selenocyanates and diselenides bearing interesting bioactive scaffolds (quinoline, quinoxaline, acridine, chromene, furane, isosazole, etc.) was synthesized, their in vitro leishmanicidal activities against Leishmania infantum amastigotes along with cytotoxicities human THP-1 cells were determined. Interestingly, most tested compounds active the low micromolar range led us to identify four lead (1h, 2d, 2e, 2f) 50% effective dose (ED 50 ) values ranging from 0.45...
Journal Article A head-to-tail tandem organization of hsp70 genes in Trypanosoma cruzi Get access Jose María Requena, Requena Centro de Biología Molecular, Consejo Superior Investigaciones Cientificas, Universidad Autónoma MadridMadrid 28049, Spain Search for other works by this author on: Oxford Academic PubMed Google Scholar Manuel Carlos López, López Antonio Jimnez-Ruiz, Jimnez-Ruiz Juan la Torre, Torre Alonso * *To whom correspondence should be addressed Nucleic Acids Research, Volume...
Abstract Membrane glycoconjugates on the Leishmania parasites, notably leishmanolysin and lipophosphoglycan, have been implicated in attachment invasion of host macrophages. However, function parasite surface Ag 2 (PSA-2) membrane proteophosphoglycan (PPG) has not elucidated. In this study we demonstrate that native recombinant infantum PSA-2, which consists predominantly 15 leucine-rich repeats (LRR) a LRR domain derived from L. major PPG, bind to The interaction is restricted macrophages...
A novel series of thirty-one N-substituted urea, thiourea, and selenourea derivatives containing diphenyldiselenide entities were synthesized, fully characterized by spectroscopic analytical methods, screened for their in vitro leishmanicidal activities. The cytotoxic activity these was tested against Leishmania infantum axenic amastigotes, selectivity assessed human THP-1 cells.
A series of 9-mer and 13-mer amide-bridged cyclic peptides derived from the linear prototype Ac-PKIIQSVGIS-Nle-K-Nle-NH2 (Toro et al. ChemBioChem 2013) has been designed synthesized by introduction lactam between amino acid side chains that are separated one helical turn (i, i+4). All these compounds were tested in vitro as both dimerization enzyme inhibitors Leishmania infantum trypanothione reductase (Li-TryR). Three peptide derivatives (3, 4 6) inhibited oxidoreductase activity Li-TryR...
Disruption of protein–protein interactions essential oligomeric enzymes by small molecules represents a significant challenge. We recently reported some linear and cyclic peptides derived from an α-helical region present in the homodimeric interface Leishmania infantum trypanothione reductase (Li-TryR) that showed potent effects on both dimerization redox activity this enzyme. Here, we describe our first steps toward design nonpeptidic small-molecule Li-TryR disruptors using proteomimetic...
Inhibition of Leishmania infantum trypanothione disulfide reductase (LiTryR) by disruption its homodimeric interface has proved to be an alternative and unexploited strategy in the search for novel antileishmanial agents. Proof concept was first obtained peptides peptidomimetics. Building on previously reported dimerization disruptors containing imidazole-phenyl-thiazole scaffold, we now report a new 1,2,3-triazole-based chemotype that yields noncompetitive, slow-binding inhibitors LiTryR....
Binding at the interface: We tested inhibitory activity of a set peptide sequences derived from an α-helix dimeric trypanothione reductase Leishmania infantum. Replacement glutamic acid residue with lysine promoted monomer dissociation and enzyme inhibition. As service to our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may be re-organized for online delivery, but not copy-edited or typeset. Technical support issues...
To improve the potential of trifluralin (TFL) in management Leishmania infantum infections through synthesis analogs (TFLA) and incorporation nanoparticulate drug delivery systems (NanoDDS), liposomes solid lipid nanoparticles, for selective targeting to leishmania infection sites.In vitro screening 18 TFLA was performed by flow cytometry. NanoDDS were loaded with active evaluated antileishmanial efficacy mice determination parasite burden liver spleen.The testing revealed most nontoxic...