A5045766587- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Child and Adolescent Psychosocial and Emotional Development
- Monoclonal and Polyclonal Antibodies Research
- Mental Health Research Topics
- Immune Cell Function and Interaction
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- Respiratory viral infections research
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- T-cell and B-cell Immunology
- Influenza Virus Research Studies
- Antifungal resistance and susceptibility
- Yersinia bacterium, plague, ectoparasites research
- HIV/AIDS Research and Interventions
- Adolescent and Pediatric Healthcare
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Personality Disorders and Psychopathology
- Resilience and Mental Health
- Animal Virus Infections Studies
- Bacillus and Francisella bacterial research
- Schizophrenia research and treatment
Intralytix (United States)
2021-2024
University of Colorado Boulder
2018-2021
Profectus Biosciences (United States)
2015-2019
University of Baltimore
2015
University of Maryland, Baltimore
2013-2015
Yale University
2007-2011
University of California, Berkeley
2008
Biotechnology Institute
2006
Columbia University
2001-2005
Emory University
1993-2000
Significance Our candidate HIV vaccine, a single-chain gp120-CD4 chimera, elicits protection against acquisition of simian-human immunodeficiency virus (SHIV)/simian (SIV) in rhesus macaques. Antibody-dependent cellular cytotoxicity was an inverse correlate infection risk. However, it is attenuated when antigen-specific T-cell responses exceed threshold, presumably due to the generation CD4+ CCR5+ T cells, preferred targets SHIV/SIV. Multiple studies strongly suggest that HIV/SIV-specific...
Candida albicans , the major invasive fungal pathogen of humans, can cause both debilitating mucosal infections and fatal infections. Understanding complex nature host–pathogen interaction in each these contexts is essential to developing desperately needed therapies treat RNA-seq enables a systems-level understanding infection by facilitating comprehensive analysis transcriptomes from multiple species (e.g., host pathogen) simultaneously. We used characterize C. human endothelial cells or...
Background Vulvovaginal candidiasis is commonly diagnosed and has been associated in prospective studies with the acquisition of HIV. Little data available on how composition vaginal microbiota, other risk factors, are molecular detection Candida albicans —a common cause vulvovaginal candidiasis. Methods In a cross-sectional study, self-collected swabs were obtained from 394 nonpregnant, reproductive-age women. C. was detected using polymerase chain reaction targeting ITS1/2 region. Vaginal...
ABSTRACT The CTC series of cobalt chelates display in vitro and vivo activity against herpes simplex virus types 1 2 (HSV-1 HSV-2). experiments described here identify the stage life cycle where CTC-96 acts demonstrate that drug inhibits infection susceptible cells. at 50 μg/ml has no effect on adsorption virions to Vero cell monolayers. Penetration assays reveal entry independent gC cellular receptors. This observation was supported by failure detect accumulation virus-specified proteins α...
Bacterial transport of many sugars, coupled to their phosphorylation, is carried out by the phosphoenolpyruvate (PEP):sugar phosphotransferase system and involves five phosphoryl group transfer reactions. Sugar translocation initiates with Mg(2+)-dependent phosphorylation enzyme I (EI) PEP. Crystals Escherichia coli EI were obtained mixing protein Mg(2+) PEP, followed oxalate, an inhibitor. The crystal structure reveals a dimeric where each subunit comprises three domains: domain that binds...
Examined the role of attributional style in adolescent's psychological functioning. Specifically, we examined cross-sectional correlates style, as well changes over time. A sample 841 adolescents with either maladaptive or adaptive styles completed a battery self-report measures at 2 points time, 1 year apart. Measures assessed depressive symptoms and suicidality, cognitive functioning (self-esteem, pessimism, coping skills), interpersonal (social competence, conflict parents, social support...
Abstract The recombinant ALVAC vaccine coupled with the monomeric gp120/alum protein have decreased risk of HIV and SIV acquisition. Ab responses to V1/V2 regions correlated a virus acquisition in both humans macaques. We hypothesized that breadth functional profile Abs induced by an ALVAC/envelope regimen could be improved substituting gp120 boost, full-length single-chain (FLSC) protein. FLSC is CD4-gp120 fusion immunogen exposes cryptic epitopes immune system. compared immunogenicity...
ABSTRACT The emergence in 1997 and continuance today of a highly lethal H5N1 avian influenza virus (AIV) causing human disease has raised concern about an impending pandemic the need for vaccine to prepare such occurrence. We previously generated efficacious vesicular stomatitis (VSV)-based AIV expressing H5 hemagglutinin (HA) from fifth genomic position VSV (J. A. Schwartz et al., Virology 366:166-173, 2007). Here we have characterized VSV-based vaccines that express A/Hong Kong/156/1997...
Copper is an essential nutrient that toxic to cells when present in excess. The fungal pathogen Candida albicans employs several mechanisms survive the presence of excess copper, but molecular pathways govern these responses are not completely understood. We report deletion GPA2, which specifies a G-protein α subunit, confers increased resistance copper and propose due combination decreased uptake increase chelation by metallothioneins. This supported our observations gpa2Δ/Δ mutant has...
ABSTRACT Yersinia pestis, one of the deadliest bacterial pathogens ever known, is responsible for three plague pandemics and several epidemics, with over 200 million deaths during recorded history. Due to high genomic plasticity, Y. pestis amenable genetic mutations as well engineering that can lead emergence or intentional development pan-drug-resistant strains. Indeed, antibiotic-resistant strains (e.g., carrying multidrug-resistant MDR plasmids) have been isolated in various countries...
ABSTRACT We analyzed the ability of a vaccine vector based on vesicular stomatitis virus (VSV) to induce neutralizing antibody (NAb) response avian influenza viruses (AIVs) in rhesus macaques. Animals vaccinated with vectors expressing either strain A/Hong Kong/156/1997 or A/Vietnam/1203/2004 H5 hemagglutinin (HA) were able generate robust NAb responses. The NAbs against homologous and heterologous AIVs after single dose was dependent upon HA antigen incorporated into VSV vaccine. superior...
Article2 May 2019Open Access Transparent process Antibody Fab-Fc properties outperform titer in predictive models of SIV vaccine-induced protection Srivamshi Pittala Department Computer Science, Dartmouth, Hanover, NH, USA Search for more papers by this author Kenneth Bagley Profectus BioSciences, Inc., Baltimore, MD, Jennifer A Schwartz Eric P Brown Thayer School Engineering, Joshua Weiner Ilia J Prado Wenlei Zhang Rong Xu Ayuko Ota-Setlik Ranajit Pal Advanced Bioscience Laboratories,...
Nontyphoidal Salmonella bacteria are the causative agent of salmonellosis, which accounts for majority foodborne illness bacterial etiology in humans. Here, we demonstrate safety and efficacy prophylactic administration a bacteriophage preparation termed FOP (foodborne outbreak pill), contains lytic phages targeting (SalmoFresh phage cocktail), Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes, lowering burdens OMM12 gnotobiotic mice. Prophylactic significantly reduced...
A double-blind, placebo-controlled study was conducted to assess the relative efficacy and tolerability of fluoxetine desipramine in depressed, human immunodeficiency virus (HIV)-positive women. Although difficulty recruitment retention participants led insufficient power detect differences between treatment groups, results indicated that experienced improvement their depression. However, for most women, significant depressive symptoms remained after 6 weeks treatment. In addition, although...
The pseudorabies virus (PRV) UL54 homologs are important multifunctional proteins with roles in shutoff of host protein synthesis, transactivation and cellular genes, regulation splicing translation. Here we describe the first genetic characterization UL54. We constructed null mutations a PRV bacterial artificial chromosome using sugar suicide lambdaRed allele exchange systems. Surprisingly, is dispensable for growth tissue culture but exhibits small-plaque phenotype that can be complemented...
ABSTRACT Yersinia pestis , one of the deadliest bacterial pathogens ever known, is responsible for three plague pandemics and several epidemics, with over 200 million deaths during recorded history. Due to high genomic plasticity, Y. amenable genetic mutations as well engineering that can lead emergence or intentional development pan-drug resistant strains. The dissemination such strains could be catastrophic, public health consequences far more daunting than those caused by recent COVID-19...
Vaccination is a proven intervention against human viral diseases; however, success Herpes Simplex Virus 2 (HSV-2) remains elusive. Most HSV-2 vaccines tested in humans to date contained just one or two immunogens, such as the virion attachment receptor glycoprotein D (gD) and/or envelope fusion protein, B (gB). At least three factors may have contributed failures of subunit-based vaccines. First, immune responses directed antigens lack sufficient antigenic breadth for efficacy. Second,...
DNA encoded adjuvants are well known for increasing the magnitude of cellular and/or humoral immune responses directed against vaccine antigens. can also tune antigens to better protect infection target organism. Two potent that have unique abilities catalytic A1 domains Cholera Toxin (CTA1) and Heat-Labile Enterotoxin (LTA1). Here, we characterized adjuvant activities CTA1 LTA1 using HIV SIV genes as model Both these enhanced antigen-specific on par with those induced by well-characterized...