A5055661364- Ion channel regulation and function
- Connexins and lens biology
- Cardiovascular Effects of Exercise
- Neuroscience of respiration and sleep
- Genetic Neurodegenerative Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Adipose Tissue and Metabolism
- Spaceflight effects on biology
- Advanced Memory and Neural Computing
- Neuroscience and Neural Engineering
- Microtubule and mitosis dynamics
- Nanopore and Nanochannel Transport Studies
- Neuroscience and Neuropharmacology Research
- Erythrocyte Function and Pathophysiology
- Cellular transport and secretion
- Physiological and biochemical adaptations
Université Claude Bernard Lyon 1
2020-2023
Inserm
2020-2023
Centre National de la Recherche Scientifique
2020-2023
Institut NeuroMyoGène
2020-2023
University of Chile
2021
Mutations in the BIN1 (Bridging Interactor 1) gene, encoding membrane remodeling protein amphiphysin 2, cause centronuclear myopathy (CNM) associated with severe muscle weakness and myofiber disorganization hypotrophy. There is no available therapy, validation of therapeutic proof concept impaired by lack a faithful easy-to-handle mammalian model. Here, we generated characterized Bin1mck-/- mouse through Bin1 knockout skeletal muscle. mice were viable, unlike constitutive knockout, displayed...
Tight control of skeletal muscle contractile activation is secured by the excitation-contraction (EC) coupling protein complex, a molecular machinery allowing plasma membrane voltage to activity ryanodine receptor Ca2+ release channel in sarcoplasmic reticulum (SR) membrane. This has been shown be intimately linked pannexin-1 (Panx1). We investigated whether prescription drug probenecid, widely used Panx1 blocker, affects signaling, EC coupling, and force. The effect probenecid was tested on...
TRAAK channels are mechano-gated two-pore-domain K + channels. Up to now, activity of these has been reported in neurons but not skeletal muscle, yet an archetype tissue challenged by mechanical stress. Using patch clamp methods on isolated muscle fibers from adult zebrafish, we show here that single sharing properties channels, i.e., selective ions, 56 pS unitary conductance the presence 5 mM external , activated membrane stretch, heat, arachidonic acid, and internal alkaline pH, present...
One of the most important functions skeletal muscle is to respond nerve stimuli by contracting. This function ensures body movement but also participates in other physiological roles, like regulation glucose homeostasis. Muscle activity closely regulated adapt different demands and shows a plasticity that relies on both transcriptional stimuli. These two processes, dependent depolarization plasma membrane, have so far been regarded as separated independent processes due lack evidence common...
Pannexins are plasma membrane heptameric channels mediating ATP release from the cytosol to extracellular space. Skeletal muscle activity is associated with Pannexin 1 (Panx1) activation, out space and subsequent activation of purinergic signaling pathways. In agreement, recent evidence has shown molecular functional interactions between Panx1 excitation-contraction (EC) coupling machinery skeletal muscle. this framework, we tested whether pharmacological effectors affect EC in...