A5003913893- Gut microbiota and health
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- IL-33, ST2, and ILC Pathways
- Parasites and Host Interactions
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Digestive system and related health
- Pancreatic and Hepatic Oncology Research
- Fibroblast Growth Factor Research
- Cancer Immunotherapy and Biomarkers
- Listeria monocytogenes in Food Safety
- Therapeutic Uses of Natural Elements
- CRISPR and Genetic Engineering
- Complementary and Alternative Medicine Studies
- Parasitic infections in humans and animals
- Gastrointestinal motility and disorders
- Mast cells and histamine
- CAR-T cell therapy research
- Immune cells in cancer
- Asthma and respiratory diseases
- Clostridium difficile and Clostridium perfringens research
University of Glasgow
2018-2024
Institute of Infection and Immunity
2021
Macrophages in the healthy intestine are highly specialized and usually respond to gut microbiota without provoking an inflammatory response. A breakdown this tolerance leads bowel disease (IBD), but mechanisms by which intestinal macrophages normally become conditioned promote microbial unclear. Strong epidemiological evidence linking disruption of antibiotic use early life IBD indicates important role for modulating immunity. Here, we show that causes hyperresponsive bacterial stimulation,...
The gut microbiota's function in regulating health has seen it linked to disease progression several cancers. However, there is limited research detailing its influence breast cancer (BrCa). This study found that antibiotic-induced perturbation of the microbiota significantly increases tumor multiple BrCa mouse models. Metagenomics highlights common loss bacterial species following antibiotic administration. One such bacteria,
The intestinal lamina propria contains a diverse network of fibroblasts that provide key support functions to cells within their local environment. Despite this, our understanding the diversity, location and ontogeny along length intestine remains incomplete. Here we show small large contain similar fibroblast subsets locate in specific anatomical niches. Nevertheless, find transcriptional profile differs markedly between colon suggesting region functions. We perform vivo transplantation...
Type 2 immunity is activated in response to both allergens and helminth infection. It can be detrimental or beneficial, there a pressing need better understand its regulation. The immunosuppressive cytokine IL-10 known as T helper (Th2) effector molecule, but it currently unclear whether dampens promotes Th2 differentiation during Here we show that infection mice elicits expression the intestinal lamina propria draining mesenteric lymph node, with higher infected tissue. In vitro, exogenous...
Abstract Background Breast cancer is the second most prevalent worldwide with around 1.7 million new cases diagnosed every year. Whilst prognosis generally favourable in early stages, this worsens significantly advanced disease. Therefore, it pertinent to focus on mitigating factors that may slow growth or progression. Recently, gut microbiome has been implicated a wide-range of roles tumour biology. Through modulation immunity, microbiota can improve efficacy several immunotherapies....
Colorectal cancer (CRC) is the third most prevalent worldwide with a high mortality rate (20-30%), especially due to metastasis adjacent organs. Clinical responses chemotherapy, radiation, targeted and immunotherapies are limited subset of patients making metastatic CRC (mCRC) difficult treat. To understand therapeutic modulation immune response in mCRC, we have used genetically engineered mouse model (GEMM), "KPN", which resembles human 'CMS4'-like subtype. We show here that transforming...
Conventional dendritic cells (cDC) are central to maintaining the balance between protective immune responses and tolerance harmless antigens, especially in intestine. Short chain fatty acids (SCFAs) such as butyrate play critical roles regulating intestinal immunity, but underlying mechanisms remain unclear. Here we demonstrate that microbiota-derived alters cDC populations vivo resulting decreased numbers of cDC2 lineage. By establishing a novel vitro culture model, show has direct...
While immune responses have been rigorously examined after intravenous Listeria monocytogenes (Lm) infection, less is understood about its dissemination from the intestines or induction of adaptive immunity more physiologic models foodborne infection. Consequently, this study focused on early events in intestinal mucosa and draining mesenteric lymph nodes (MLN) using infection mice with Lm modified to invade murine epithelium (InlAM Lm). InlAM trafficked intracellularly MLN were associated...
The use of helminth infections as tools to understand the type 2 immune response is a well-established technique and important many areas immunological research. phenotype function cell populations at site infection key determinant pathogen clearance. However, with helminths such murine nematode Heligomosmoides polygryrus cause increased mucus production thickening intestinal wall, which can result in extensive death when isolating analysing cells from lamina propria (LP). Populations larger...
Abstract The diverse community of commensal microbes that comprise the gut microbiota is known to play an integral role in human health, not least through its ability regulate host immune responses and metabolic pathways. Alterations homeostasis this community, including use broad-spectrum antibiotics, have already been associated with progression several cancers, namely melanoma liver. aggressive nature breast cancer (BrCa), largely due metastasize early, has ranked disease second highest...
Abstract The intestinal immune system maintains a balance between active immunity needed for protection and tolerance towards harmless antigens. Dendritic cells (DCs) found in the mucosa are key to adaptive arm of these immunoregulatory events. DCs sample antigens tissue then migrate draining lymph nodes, where they prime T that back as effector or regulatory cells. Intestinal DC highly heterogeneous, it remains unclear exactly which subsets induces different kinds response, what signalling...
The experiments presented here are based on the reconfiguration of an ancient medicine, Lemnian Earth (LE) ( terra sigillata , stamped earth sphragis ), acclaimed therapeutic clay with a 2500-year history use. Based our hypothesis that LE was not natural material but artificially modified one involving clay-fungus interaction, we present results from co-culture common fungus, Penicillium purpurogenum Pp two separate slurries, smectite and kaolin, which principal constituents LE. Our show:...
Type 2 immunity is activated in response to both allergens and helminth infection. It can be detrimental or beneficial, there a pressing need better understand its regulation. The immunosuppressive cytokine IL-10 known as T helper (Th2) effector molecule, but it currently unclear whether dampens promotes Th2 differentiation during Here we show that infection mice elicits expression the intestinal lamina propria draining mesenteric lymph node, with higher infected tissue. In vitro, exogenous...
Summary Intestinal fibroblasts (FB) play essential roles in intestinal homeostasis. Here we show that the small and large lamina propria (LP) contain similar FB subsets locate specific anatomical niches express distinct arrays of epithelial support genes. However, there were tissue differences transcriptional profile two sites. All adult LP mesenchymal stromal cells (MSC), including FB, smooth muscle (SMC) pericytes derive from Gli1 -expressing embryonic precursors which identify as...
Abstract The use of helminth infections as tools to understand the type 2 immune response is a well-established technique and important many areas immunological research. phenotype function cell populations at site infection key determinant pathogen clearance. However, with helminths such murine nematode Heligomosmoides polygryrus cause increased mucus production thickening intestinal wall, which can result in extensive death when isolating analysing cells from lamina propria (LP)....
Abstract Intestinal fibroblasts (FB) play essential roles in intestinal homeostasis. Here we show that the small and large lamina propria (LP) contain similar FB subsets locate specific anatomical niches express distinct arrays of epithelial support genes. However, there were tissue differences transcriptional profile two sites. All adult LP mesenchymal stromal cells (MSC), including FB, smooth muscle (SMC) pericytes derive from Gli1 -expressing embryonic precursors which identify as...