A5089380082- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Yersinia bacterium, plague, ectoparasites research
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Pharmacological Effects of Natural Compounds
- Listeria monocytogenes in Food Safety
- Cancer Immunotherapy and Biomarkers
- S100 Proteins and Annexins
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Transgenic Plants and Applications
- Atherosclerosis and Cardiovascular Diseases
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Bacterial Infections and Vaccines
- vaccines and immunoinformatics approaches
- Biochemical Analysis and Sensing Techniques
- Antibiotic Resistance in Bacteria
- Helicobacter pylori-related gastroenterology studies
- Immune responses and vaccinations
- T-cell and Retrovirus Studies
Stony Brook University
2016-2024
NYU Langone Health
2022-2024
Stony Brook School
2020-2024
Immunology from Concept and Experiments to Translation
2015-2020
Centre National de la Recherche Scientifique
2013-2017
Université de Bordeaux
2013-2017
Significance Human γδ T lymphocytes have innate-like and adaptive-like functions can circulate in blood or reside tissues. They are activated by specific antigens recognized their T-cell receptor recognize infected transformed cells, suggesting that cellular stress is involved antigen expression. However, molecular characterization of stress-induced remains elusive, hampering our understanding the role cells cancer infections. In present study we identify annexin A2 as such known a...
CD8 tissue-resident memory T (TRM) cells provide frontline protection at barrier tissues; however, mechanisms regulating TRM cell development are not completely understood. Priming dictates the migration of effector to tissue, while factors in tissue induce situ differentiation. Whether priming also regulates differentiation uncoupled from is unclear. Here, we demonstrate that mesenteric lymph nodes (MLN) CD103+ intestine. In contrast, primed spleen were impaired ability differentiate into...
Hypervirulent Klebsiella pneumoniae (hvKp) strains are predicted to become a major threat in Asia if antibiotic resistance continues spread. Anticapsular antibodies (Abs) were developed because disseminated infections caused by hvKp associated with significant morbidity and mortality, even antibiotic-sensitive strains. K1-serotype polysaccharide capsules (K1-CPS) expressed the majority of In this study, K1-CPS-specific IgG Abs generated conjugation K1-CPS immunogenic anthrax protective...
Cytomegalovirus (CMV) is a leading infectious cause of morbidity in immune-compromised patients. γδ T cells have been involved the response to CMV but their role protection has not firmly established and dependency on other lymphocytes addressed. Using C57BL/6 αβ and/or cell-deficient mice, we here show that are as competent protect mice from CMV-induced death. cell-mediated control viral load prevented organ damage. cell recovery by bone marrow transplant or adoptive transfer experiments...
Genetically engineered mouse models (GEMMs) help us to understand human pathologies and develop new therapies, yet faithfully recapitulating diseases in mice is challenging. Advances genomics have highlighted the importance of non-coding regulatory genome sequences, which control spatiotemporal gene expression patterns splicing many diseases1,2. Including extensive genomic regions, requires large-scale engineering, should enhance quality disease modelling. Existing methods set limits on size...
Abstract The basic leucine zipper transcription factor ATF-like 3 (BATF3) is required for the development of conventional type 1 dendritic cells that are essential cross-presentation and CD8 T cell–mediated immunity against intracellular pathogens tumors. However, whether BATF3 intrinsically regulates cell responses not well studied. In this article, we report a role cell-intrinsic Batf3 expression in regulating establishment circulating resident memory after foodborne Listeria monocytogenes...
Abstract Gammaherpesviruses are oncogenic viruses that establish lifelong infections and significant causes of morbidity mortality. Vaccine strategies to limit gammaherpesvirus infection disease in development, but there no FDA-approved vaccines for Epstein-Barr or Kaposi sarcoma herpesvirus. As a new approach vaccination, we developed tested replication-deficient virus (RDV) platform, using murine 68 (MHV68), well-established mouse model pathogenesis studies preclinical therapeutic...
Murine Ly6Chi inflammatory monocytes (IMs) require CCR2 to leave the bone marrow and enter mesenteric lymph nodes (MLNs) other organs in response Yersinia pseudotuberculosis infection. We are investigating how IMs, which can differentiate into CD11c+ dendritic cells (DCs), contribute innate adaptive immunity Y. Previously, we obtained evidence that IMs important for a dominant CD8+ T cell epitope YopE69-77 host survival using intravenous infections with attenuated Here challenged CCR2+/+ or...
Cancers associated with the oncogenic gammaherpesviruses, Epstein-Barr virus and Kaposi sarcoma herpesvirus, are notable for their constitutive activation of transcription factor signal transducer activator 3 (STAT3). To better understand role STAT3 during gammaherpesvirus latency B cell response to infection, we used model pathogen murine 68 (MHV68). Genetic deletion in cells CD19cre/+Stat3f/f mice reduced peak MHV68 approximately sevenfold. However, infected exhibited disordered germinal...
While immune responses have been rigorously examined after intravenous Listeria monocytogenes (Lm) infection, less is understood about its dissemination from the intestines or induction of adaptive immunity more physiologic models foodborne infection. Consequently, this study focused on early events in intestinal mucosa and draining mesenteric lymph nodes (MLN) using infection mice with Lm modified to invade murine epithelium (InlAM Lm). InlAM trafficked intracellularly MLN were associated...
Neonatal thymectomy in BALB/c mice has been described as a model of gastric mucosa-associated lymphoid tissue (MALT) lymphoma (GML). By using this experimental system, we screened, for the first time to our knowledge, Helicobacter pylori GML-associated strains their capacity promote disease. A cohort underwent at day 3 after birth (d3Tx). Successful thymic ablation was evaluated by degree lymphopenia blood samples collected 4 weeks age. d3Tx and non-thymectomized controls were infected with...
Cancers associated with the oncogenic gammaherpesviruses, Epstein-Barr virus and Kaposi sarcoma herpesvirus, are notable for their constitutive activation of transcription factor STAT3. To better understand role STAT3 during gammaherpesvirus latency immune control, we utilized murine 68 (MHV68) infection. Genetic deletion in B cells CD19cre/+Stat3f/f mice reduced peak approximately 7-fold. However, infected exhibited disordered germinal centers heightened virus-specific CD8 T cell responses...
Human cytomegalovirus (HCMV) components are often found in tumors, but the precise relationship between HCMV and cancer remains a matter of debate. Pro-tumor functions were described several studies, an association seropositivity reduced risk was also evidenced, presumably relying on recognition killing cells by HCMV-induced lymphocytes. This study aimed at deciphering whether CMV influences development immune-independent manner. Using immunodeficient mice, we showed that systemic infection...
Abstract Background It is generally accepted that aging has detrimental effects on conventional T cell responses to systemic infections. However, most pathogens naturally invade the body through mucosal barriers. Although sites are highly enriched in unconventional immune sentinels like γδ cells, little currently known about impact of responses. We previously established foodborne infection with a mouse-adapted internalin A mutant Listeria monocytogenes ( Lm ) generates an adaptive...
Abstract Genetically Engineered Mouse Models (GEMMs) aid in understanding human pathologies and developing new therapeutics, yet recapitulating diseases authentically mice is challenging to design execute. Advances genomics have highlighted the importance of non-coding regulatory genome sequences controlling spatiotemporal gene expression patterns splicing diseases. It thus apparent that including genomic regions during engineering GEMMs highly preferable for disease modeling, with...
Influenza viruses are a major global cause of morbidity and mortality. Vagal TRPV1
Abstract Age-related inflammation or ‘inflammaging’ is a key mechanism that increases disease burden and may control lifespan. How adipose tissue macrophages (ATMs) inflammaging not well understood in part because the molecular identities of niche-specific ATMs are incompletely known. Using intravascular labeling to exclude circulating myeloid cells subsequent single-cell sequencing with orthogonal validation, we define diversity alterations niche resident through Aging led depletion...