A5055084957- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- Inflammatory Biomarkers in Disease Prognosis
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Advanced Biosensing Techniques and Applications
- Caveolin-1 and cellular processes
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Peptidase Inhibition and Analysis
- vaccines and immunoinformatics approaches
- Cancer Cells and Metastasis
- Salivary Gland Tumors Diagnosis and Treatment
- Cancer-related gene regulation
- S100 Proteins and Annexins
- Biosensors and Analytical Detection
Centro de Investigaciones Biológicas Margarita Salas
2012-2016
Municipal Institute for Medical Research
2013
Consejo Superior de Investigaciones Científicas
2013
Abstract Purpose: Cancer-associated fibroblasts (CAF) are essential components of the stroma that play a critical role in cancer progression. This study aimed to identify novel CAFs markers might contribute invasion and prognosis colorectal cancer. Experimental Design: The azoxymethane/dextran sodium sulfate mouse model sporadic colon represents an adequate source for isolation normal fibroblasts. By using explants technique, we purified from tissues. Whole-cell extracts supernatants were...
Liver metastasis in colorectal cancer is the major cause of cancer-related deaths. To identify and characterize proteins associated with colon metastasis, we have compared conditioned serum-free medium highly metastatic KM12SM cells parental, poorly KM12C using quantitative stable isotope labeling by amino acids cell culture (SILAC) analyses on a linear ion trap-Orbitrap Velos mass spectrometer. In total, 1337 were simultaneously identified SILAC forward reverse experiments. For...
Humoral response in cancer patients can be used for early detection. By screening high‐density protein microarrays with sera from colorectal (CRC) and controls, we identified 16 tumor‐associated antigens (TAAs) exhibiting high diagnostic value. This number of TAAs requires the development multiplex assays combining different a faster more accurate prediction CRC. Here, have developed optimized bead‐based assay using nine selected two controls to provide test CRC diagnosis. We screened...
Although autoantibody detection has been proposed for diagnosis of colorectal cancer, little is known about their initial production and development correlation with cancer progression. Azoxymethane/dextran sodium sulfate (AOM/DSS)-treated mice developed colon adenocarcinoma in the distal similar to human sporadic cancer. We assessed this model together AOM DSS-only models applicability early All AOM/DSS-treated produced autoantibodies tumor-associated antigens analogous those observed...
<p>PDF file - 88K</p>
<p>PDF file - 338K, Supplementary Figures S1 (representation of iTRAQ workflow), S2 (data normalization for analysis), S3 (expression CAF markers in colorectal tumors mice), S4 and S5 (correlations marker expression with clinical data)</p>
<p>PDF file - 88K</p>
<p>PDF file - 338K, Supplementary Figures S1 (representation of iTRAQ workflow), S2 (data normalization for analysis), S3 (expression CAF markers in colorectal tumors mice), S4 and S5 (correlations marker expression with clinical data)</p>
<div>Abstract<p><b>Purpose:</b> Cancer-associated fibroblasts (CAF) are essential components of the stroma that play a critical role in cancer progression. This study aimed to identify novel CAFs markers might contribute invasion and prognosis colorectal cancer.</p><p><b>Experimental Design:</b> The azoxymethane/dextran sodium sulfate mouse model sporadic colon represents an adequate source for isolation normal fibroblasts. By using explants...
<div>Abstract<p><b>Purpose:</b> Cancer-associated fibroblasts (CAF) are essential components of the stroma that play a critical role in cancer progression. This study aimed to identify novel CAFs markers might contribute invasion and prognosis colorectal cancer.</p><p><b>Experimental Design:</b> The azoxymethane/dextran sodium sulfate mouse model sporadic colon represents an adequate source for isolation normal fibroblasts. By using explants...