A5089096604- High Temperature Alloys and Creep
- Esophageal Cancer Research and Treatment
- Gastric Cancer Management and Outcomes
- Fatigue and fracture mechanics
- Esophageal and GI Pathology
- Microstructure and Mechanical Properties of Steels
- Cardiomyopathy and Myosin Studies
- Fire effects on concrete materials
- Lung Cancer Diagnosis and Treatment
- Material Properties and Failure Mechanisms
- Nutrition and Health in Aging
- Epigenetics and DNA Methylation
- Metastasis and carcinoma case studies
- Nuclear Materials and Properties
- Gastrointestinal Tumor Research and Treatment
- Genetic factors in colorectal cancer
- Advanced Materials Characterization Techniques
- Protein Kinase Regulation and GTPase Signaling
- Muscle Physiology and Disorders
- Enzyme Structure and Function
- Cancer-related gene regulation
- Metallurgy and Material Forming
- Cancer Genomics and Diagnostics
- Molecular Junctions and Nanostructures
- Cancer Immunotherapy and Biomarkers
The University of Tokyo
2016-2025
University of Science and Technology Beijing
2014-2023
University of Tokyo Hospital
2020-2022
Japan Society for the Promotion of Science
2020
Chiba University
2015
Showa General Hospital
2011
Saitama Cancer Center
2009
National Institute for Materials Science
1998-2007
Tokyo Metropolitan Neurological Hospital
2002-2003
Institute of Metal Research
1992-2001
Myosin light chain kinase which phosphorylates g, of skeletal muscle myosin requires an activator for the activity (Yazawa, M., and Yagi, K (1977) J. Biochem.(Tokyo) 82, 287-289).This has now been identified as modulator protein known to be a Ca*+-dependent regulator phosphodiesterase, adenylate cyclase, ATPases.The identification is based on quantitative cross-reactivity brain in activating phosphodiesterase identical properties both proteins regard sensitivities Ca"+, UV absorption...
Invertebrate calmodulins of the sea anemone and scallop muscle were isolated their properties compared with those vertebrate from rabbit pig brain. The molecular weights estimated by SDS-polyacrylamide gel electrophoresis similar to weight (16,500) calmodulins. Every calmodulin contained 1 mol each trimethyllysine histidine, high contents acidic amino acids. marine invertebrate only one tyrosine in contrast two tyrosines ones. As a result, UV absorption spectra clearly different....
Whereas the CpG island methylator phenotype (CIMP) in colorectal cancer associates with microsatellite instability (MSI)-high and BRAF-mutation(+), existence of an intermediate-methylation subgroup associated KRAS-mutation(+) is controversial, suitable markers for have yet to be developed. Our aim clarify DNA methylation epigenotypes more comprehensively.To select new on a genome-wide scale, we did methylated immunoprecipitation-on-chip analysis cell lines re-expression array by...
Res. Commun. 90,261-268). This protein has now been identified as the modulator protein.The identification was based on capability of this to activate brain modulator-deficient phosphodiesterase and mobility upon polyacrylamide gel electrophoresis.The activation guanylate cyclase specifically attributable Tetrahymena since other proteins examined (bovine brain, sea anemone, scallop) were ineffective.Under conditions where occurred, activities from sources, that include rat lung, human...
Chronic infections by hepatitis B virus (HBV) and C (HCV) appear to be the most significant causes of hepatocellular carcinoma (HCC). Aberrant promoter methylation is known deeply involved in cancer, including HCC. In this study, we analyzed aberrant methylated DNA immunoprecipitation‐on‐chip analysis on a genome‐wide scale six HCCs three HBV‐related HCV‐related HCCs, matched noncancerous liver tissues, normal tissues. Candidate genes with were detected more frequently preferentially or...
Molecular characterisation using gene-expression profiling will undoubtedly improve the prediction of treatment responses, and ultimately, clinical outcome cancer patients.To establish procedures to identify responders FOLFOX therapy, 83 colorectal (CRC) patients including 42 41 non-responders were divided into training (54 patients) test (29 sets. Using Random Forests (RF) algorithm in set, predictor genes for therapy identified, which applied samples sensitivity, specificity, out-of-bag...
Abstract Early detection of esophageal squamous cell carcinoma (ESCC) is an important prognosticator, but difficult to achieve by conventional endoscopy. Conventional lugol chromoendoscopy and equipment-based image-enhanced endoscopy, such as narrow-band imaging (NBI), have various practical limitations. Since fluorescence-based visualization considered a promising approach, we aimed develop activatable fluorescence probe visualize ESCCs. First, based on the fact that aminopeptidase...
Transthoracic esophagectomy (TTE) is believed to have advantages for mediastinal lymphadenectomy in the treatment of resectable esophageal cancer despite its association with a greater incidence pulmonary complications and postoperative mortality. Transhiatal regarded as less invasive, though insufficient terms lymph node dissection. With aim achieving dissection equivalent that TTE, we developed nontransthoracic (NTTE) procedure combining video-assisted cervical approach upper mediastinum...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance studies on calmodulin: calcium-induced conformational changeMitsuhiko Ikura, Toshifumi Hiraoki, Kunio Hikichi, Toshiaki Mikuni, Michio Yazawa, and Koichi YagiCite this: Biochemistry 1983, 22, 10, 2573–2579Publication Date (Print):May 1983Publication History Published online1 May 2002Published inissue 10 1983https://pubs.acs.org/doi/10.1021/bi00279a039https://doi.org/10.1021/bi00279a039research-articleACS...
Ca2+ binding to calmodulin was measured in the presence of mastoparan or caldesmon fragment.Mastoparan and fragment were used as model compounds enzymes cytoskeleton proteins, respectively, working target calmodulin.Although bindings two globular domains occur independently absence peptide (or proteins), increased affinity Ca2* and, at same time, produced positive cooperative Ca" between domains.The result Cas+ compared with 'H NMR spectra equimolar concentration mastoparan.It is known that...
Modulator-deficient myosin light-chain kinase from rabbit skeletal muscle was purified by modulator protein-Sepharose 4B affinity chromatography. The protein showed a single band (MW 80,000) on polyacrylamide gel electrophoresis in sodium dodecyl sulfate, and it exists as monomer the native state determined filtration. modulator-deficient 80,000), 16,500) Ca2+ were essential for activity. half-maximal activity of presence excess with 10 mM MgCl2 at pCa 5.1, where full actomyosin-ATPase is...
This study is a new assessment of damaged roads after the Kumamoto earthquake in southern Japan (2016) using remotely sensed synthetic aperture radar (SAR) data, field data and deep learning. Three SAR images from descending orbits Sentinel-1 VV (vertical-vertical) polarizations are considered for radiometric calibration, geocoding interferometric analyses. Field terms IRI (international roughness index) were gathered over more than 530 km smartphone accelerometer BumpRecorder application....
Abstract Background Gastric cancer with peritoneal dissemination (PD) has a dismal prognosis, and current treatments have shown little efficacy. CLDN18.2-targeted therapies promising efficacy against gastric cancers that express high levels of CLDN18. Because the limited information regarding CLDN18.2 status in PD, we analyzed PD-positive for CLDN18 both primary along HER2 PD-L1 combined positive score (CPS). Methods Immunohistochemical analyses were performed on 84 cases using paired PD...
We developed a robot-assisted transmediastinal esophagectomy (RATME) to reduce the surgical invasiveness of open transthoracic (oTTE). However, long-term survival outcomes patients who undergo RATME remain unclear. Patients underwent for esophageal squamous cell carcinoma (ESCC) between January 2012 and April 2020 were enrolled. Overall (OS), relapse-free (RFS), ESCC cause-specific (CSS) analyzed according clinical stage. Survivals compared oTTE using propensity score matching analysis. One...
Abstract To investigate the usefulness of low‐dose FK506 for treatment myasthenia gravis (MG), we treated 19 patients with generalized MG in a 16‐week open clinical trial (3–5 mg/day). At end trial, total scores (range: 0–27 points) improved by 3 points or more 7 (37%), and activities daily living (ADL) 0–6 also 1 point 8 (42%). Nine (47%) showed improvement either ADL scores. Significant reduction anti‐acetylcholine receptor antibody titers interleukin 2 production were observed at this...
Fragments of scallop testis calmodulin were prepared by tryptic digestion. One peptide consisted 75 amino acid residues from N-acetylalanine to lysine at position (F12) and the other 71 aspartic 78 C-terminal (F34). Flow dialysis equilibrium experiments revealed existance two Ca2+ binding sites in each fragment. Half-saturating concentrations titration curves 11 μM for F12 3.2 F34, Hill coefficients obtained as 1.14 1.84, respectively. The results indicate that high-affinity are located on...
The complete amino acid sequence of scallop calmodulin was determined by isolating and sequencing the peptides obtained after cyanogen bromide cleavage tryptic digestion. protein consisted 148 residues its amino(N)-terminus blocked with an acetyl group. Scallop lacked tryptophan cysteine contained one mol each N-trimethyllysine (Tml) histidine per protein. only tyrosine residue, while vertebrate calmodulins contain two. On comparing that bovine calmodulin, three substitutions were found at...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance studies on calmodulin: calcium-dependent spectral change of proteolytic fragmentsMitsuhiko Ikura, Toshifumi Hiraoki, Kunio Hikichi, Osamu Minowa, Haruhiko Yamaguchi, Michio Yazawa, and Koichi YagiCite this: Biochemistry 1984, 23, 14, 3124–3128Publication Date (Print):July 3, 1984Publication History Published online1 May 2002Published inissue 3 July 1984https://doi.org/10.1021/bi00309a003RIGHTS & PERMISSIONSArticle...
Myosin light chain kinase from frozen rabbit skeletal muscle was separated into two protein components by DEAE-cellulose column chromatography. One component (MW 20,000) a Ca2+-binding protein. Both and Ca2+ were essential for the enzyme activity.
A subset of colorectal cancer shows significant accumulation aberrant promoter methylation. Previously, we developed two groups methylation markers that classified into three epigenotypes: i) high-, ii) intermediate-, and iii) low-methylation epigenotypes. High-methylation epigenotype, with both group 1 2 markers, correlates to BRAF-mutation(+). Intermediate-methylation but not 1, KRAS-mutation(+). To gain insight epigenotype development in carcinogenesis, especially intermediate-methylation...