A5050152542- Carcinogens and Genotoxicity Assessment
- Pharmacogenetics and Drug Metabolism
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Computational Drug Discovery Methods
- Eicosanoids and Hypertension Pharmacology
- Effects and risks of endocrine disrupting chemicals
- Glutathione Transferases and Polymorphisms
- Toxic Organic Pollutants Impact
- Inflammatory mediators and NSAID effects
- Air Quality and Health Impacts
- Cancer Research and Treatments
- Metal complexes synthesis and properties
- Drug-Induced Hepatotoxicity and Protection
- Amino Acid Enzymes and Metabolism
- Biotin and Related Studies
- Advanced Chemical Sensor Technologies
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Synthesis and Biological Evaluation
University of Arkansas for Medical Sciences
2016-2025
Winthrop Rockefeller Foundation
2016-2025
Harding University Main Campus
2020
Washington University in St. Louis
2020
Hendrix College
2020
University of Minnesota
2003-2020
Conway School of Landscape Design
2020
Institute of Cancer Research
2017
Arkansas Department of Health
2013
University of North Carolina at Chapel Hill
2004-2011
Abstract Traditional epitranscriptomics relies on capturing a single RNA modification by antibody or chemical treatment, combined with short-read sequencing to identify its transcriptomic location. This approach is labor-intensive and may introduce experimental artifacts. Direct of native using Oxford Nanopore Technologies (ONT) can allow for directly detecting the base modifications, although these modifications might appear as errors. The percent Error Specific Bases (%ESB) was higher than...
Purpose: The purpose of this study was to translate our in vitro therapy approach an vivo model. Increased glutamine uptake is known drive cancer cell proliferation, making tumor cells glutamine-dependent. Studying lymph-node aspirates containing malignant lung showed a strong correlation between consumption and glutathione (GSH) excretion. Subsequent experiments with A549 H460 lines provided additional evidence for glutamine's role driving synthesis excretion GSH. Using...
Non-small cell lung cancer (NSCLC) is the major form of cancer, with adenocarcinoma (LUAD) and squamous carcinoma (LUSC) being its subtypes. Smoking alone cannot completely explain etiology. We hypothesize that altered microbiome chronic inflammatory insults in tissues contribute to carcinogenesis. Here we explore composition LUAD samples, compared LUSC normal samples. Extraction DNA formalin-fixed, paraffin-embedded (FFPE) tumor adjacent was meticulously performed. The 16S rRNA product from...
1. Kurti AN, Redner R, Lopez AA, Keith DR, Stanton CA, Gaalema DE, et al. Tobacco and nicotine delivery product use in a national sample of pregnant women. Prev Med. 2017;104:50-56. doi:10.1016/j.ypmed.2017.07.030 CrossRef Google Scholar
Exhaled breath condensate (EBC) analyses promise simple and noninvasive methods to measure airway biomarkers but pose considerable methodological challenges. We utilized mass spectrometry EBC purine adenosine AMP plus urea control for dilutional variability in two studies: 1) a cross-sectional analysis of 28 healthy, 40 cystic fibrosis (CF), 11 asthmatic children; 2) longitudinal 26 CF children before after treatment pulmonary exacerbation. adenosine, AMP, were readily detected quantified by...
Toxicogenomics provides the ability to examine in greater detail underlying molecular events that precede and accompany toxicity, thus allowing prediction of adverse at much earlier times compared classical toxicological end points. Acetaminophen (APAP) is a pharmaceutical has similar metabolic toxic responses rodents humans. Recent gene expression profiling studies with APAP found an oxidative stress signature subtoxic dose we hypothesized can be phenotypically anchored conventional...
Abstract The epidermal growth factor receptor (EGFR) is highly expressed in a variety of tumors, and therefore an important biomarker for cancer diagnosis target therapy. We have developed novel peptide‐based immuno tandem mass spectrometry (iMALDI) diagnostic assay sensitive, specific, quantitative analysis EGFR, which we applied to the detection EGFR peptide three cell lines tumor biopsy sample. This capable detecting bound antibody beads at attomole levels. ability directly obtain amino...
Proteins that discriminate between cisplatin−DNA adducts and oxaliplatin−DNA are thought to be responsible for the differences in tumor range, toxicity, mutagenicity of these two important chemotherapeutic agents. However, structural basis differential protein recognition has not been determined could design more effective platinum anticancer We have high-resolution NMR structures cisplatin−GG undamaged DNA dodecamers AGGC sequence context compared with oxaliplatin−GG structure same...
Coumadin (R/S-warfarin) is a highly efficacious and widely used anticoagulant; however, its variable metabolism remains an important contributor to uncertainties in therapeutic responses. Pharmacogenetic studies report conflicting findings on the clinical relevance of CYP2C19. A resolution this controversy impeded by lack detail potential role CYP2C19 warfarin metabolism. Consequently, we assessed efficiency R- S-warfarin explored possible contributions liver using vitro methods. Recombinant...
Abstract Genetic obesity increases in liver phosphatidylcholine (PC)/phosphatidylethanolamine (PE) ratio, inducing endoplasmic reticulum (ER) stress without concomitant increase of ER chaperones. Here, it is found that exposing mice to a palm oil-based high fat (HF) diet induced obesity, loss PE, and the chaperone Grp78/BiP pericentral hepatocytes. In Hepa1–6 cells treated with elevated concentration palmitate model lipid stress, mRNA was increased, indicating onset stress-induced Unfolded...
Abstract The 2021 WHO Classification of Central Nervous System Tumors recommended evaluation cyclin‐dependent kinase inhibitor 2A/B ( CDKN2A/B ) deletion in addition to codeletion 1p/19q characterize IDH‐ mutant gliomas. Here, we demonstrated the use a nanopore‐based copy‐number variation sequencing (nCNV‐seq) approach simultaneously identify deletions and 1p/19q. nCNV‐seq was initially evaluated on three distinct glioma cell lines then applied 19 IDH ‐mutant gliomas (8 astrocytomas 11...
1,3-Butadiene is an important industrial chemical used in the production of synthetic rubber and also found gasoline combustion products. It a multispecies, multisite carcinogen rodents, with mice being most sensitive species. metabolized to several epoxides that form DNA protein adducts. Previous analysis 1,2,3-trihydroxybutyl-valine globin adducts suggested resulted from 3-butene-1,2-diol metabolism 3,4-epoxy-1,2-butanediol, rather than 1,2;3,4-diepoxybutane. To specifically examine...
Exhaled breath condensate (EBC) collection is a simple and non-invasive method to sample airway secretions, but analysis limited by extensive variable dilution of secretions within the condensate. To overcome this limitation, we developed sensitive specific liquid chromatography/tandem mass spectrometry (LC/MS/MS) simultaneously detect adenyl purines as biomarkers inflammation urea marker in EBC. Separation prior was achieved using C18 column with methanol formic acid mobile phase,...