ABSTRACT Growth heterosis is crucial for Populus deltoides breeding, a key industrial‐timber and ecological‐construction tree species in temperate regions. However, the molecular mechanisms underlying carbon (C)–nitrogen (N) metabolism coordination regulating growth remain unclear. Herein high‐hybrids of P. exhibited high‐parent mid‐parent traits enzymes C–N metabolism. In hybrids, gene expression patterns were mainly biased toward female parent. Parental contribution to differentiation,...

Introduction Enhancing the planning of forest-agricultural composite model and increasing efficiency with which forest land is utilized could benefit from a thorough understanding impacts intercropping between forests agriculture on soil physicochemical properties microbial communities. Methods Populus cathayana × candansis cv. Xinlin No.1 Glycine max intercrop soils, along their corresponding monocrops, were used in this study’s llumina high-throughput sequencing analysis to determine...

REST/NRSF is a transcriptional repressor that acts at the terminal stage of neuronal differentiation pathway and blocks transcription several genes. generally downregulated during induction differentiation. The recombinant factor REST-VP16 binds to same DNA binding site as does but functions an activator instead can directly activate target However, it not known whether expression sufficient cause formation functional neurons from neural stem cells (NSCs). Here we show regulated in...

Methyl jasmonate (MeJA) elicits stomatal closure in many plant species. Stomatal is accompanied by large ion fluxes across the plasma membrane (PM). Here, we recorded transmembrane of H(+) , Ca(2+) and K(+) guard cells wild-type (Col-0) Arabidopsis, CORONATINE INSENSITIVE1 (COI1) mutant coi1-1 PM -ATPase mutants aha1-6 aha1-7, using a non-invasive micro-test technique. We showed that MeJA induced efflux, influx efflux Col-0 cells. However, this transport was abolished cells, suggesting...

Hybrid breeding, a direct and efficient strategy for disease control management in tree species, is currently limited by the selection method of resist clones: "in vitro stem segment inoculation method". This method, constrained availability inoculating materials, cannot rapidly, efficiently, cost-effectively screen resistance all hybrid clones. To overcome these limitations, we introduce novel pathogen assessment clones poplar-Valsa sordida pathosystem. involves canker on host leaf, unique...

Recently, a novel poplar mosaic disease caused by bean common virus (BCMV) was investigated in Populus alba var. pyramidalis China. Symptom characteristics, physiological performance of the host, histopathology, genome sequences and vectors, gene regulation at transcriptional posttranscriptional levels were analyzed RT-qPCR (quantitative reverse transcription PCR) validation expression performed our experiments. In this work, mechanisms which BCMV pathogen impacts molecular response to viral...

Livestock manure and crop residues are significant sources of phosphorus. However, the ineffectiveness current processing technologies often leads to suboptimal recovery this phosphorus, causing considerable resource wastage environmental pollution. Recently, global research has increasingly been focused on organic waste materials using hydrothermal carbonization technology. This study investigated variations in phosphorus forms hydrochar produced from swine rice straw, employing diverse...

Abstract Carbon starvation is the current leading hypothesis of plant mortality mechanisms under drought stress; recently, it also used to explain tree die-off in diseases. However, molecular biology carbon pathway unclear. Here, using a punch inoculation system, we conducted transcriptome and physiological assays investigate pathogen response poplar stems at early stages Botryosphaeria Valsa canker Transcriptome showed that majority differentially expressed genes (DEGs) stem phloem xylem,...

Microbial communities associated with plants represent key determinants of plant health, survival, and growth. However, a good understanding the structural composition bacterial fungal microbiome present in different tissues growing environments, especially transgenic woody plants, is required. In study, we hypothesized that environmental conditions, ecological niches, events could influence community structure plant-associated microorganisms (bacterial endophytes). We sampled root stem...

Leaves of C. porrectum are rich in essential oils containing monoterpenes, sesquiterpenes and aromatic compounds, but the molecular mechanism terpenoid biosynthesis is still unclear. In this paper, differences contents compositions terpenoids among three chemotypes were analyzed using gas chromatography mass spectrometry (GC/MS). Furthermore, differential expression gene transcripts leaf tissues through a comparison full-length transcriptomes profiles. The oil leaves was mainly composed...

Changes in DNA methylation patterns different tissues, at various developmental stages, and under environmental stimuli have been investigated plants. However, the involvement of daily gene expression regulation plant circadian clock not reported. Here, we methylomes mRNA transcriptomes from leaves P. trichocarpa over 24 h by high-throughput sequencing. We found that approximately 15.63-19.50% genomic cytosine positions were methylated mature poplar leaves, with half being form asymmetric...

Transgenic poplar (Populus × euramericana 'Guariento') plants harboring five exogenous, stress-related genes exhibit increased tolerance to multiple stresses including drought, salt, waterlogging, and insect feeding, but the complex mechanisms underlying stress in these have not been elucidated. Here, we analyzed differences transcriptomes of transgenic line D5-20 non-transgenic D5-0 using high-throughput transcriptome sequencing techniques elucidated functions differentially expressed...

DNA methylation is an important mechanism of epigenetic modification. Methylation changes during stress responses and developmental processes have been well studied; however, their role in plant adaptation to the day/night cycle poorly understood. In this study, we detected global patterns leaves black poplar Populus nigra 'N46' at 8:00 24:00 by methylated immunoprecipitation sequencing (MeDIP-seq). We found 10,027 10,242 genes be samples, respectively. The appeared involved multiple...

Afforestation and vegetation restoration are challenging tasks in arid, semi-arid saline-alkali areas. It is now highly demanded agroforestry to generate new stress-resistant trees that adapt these difficult environments. As a high-efficiency, strong precision versatility tool, the CRISPR/Cas9 technology has been utilized broadly major crops model plants (Li et al., 2022; Zhang 2021). However, its application rarely reported, especially drought salt-tolerance fields. Hybrid proline-rich...

A key step in the study of tree pathology is identification an appropriate method for inoculating pathogens diseases branches and trunks. Pathogens trunks are commonly inoculated through punching, burning, toothpick inoculation. However, there a lack comparative analyses inoculation outcomes these three methods. In this work, six-year-old P. alba var. pyramidalis were with V. sordida using techniques to investigate differences effectiveness Results reveal that incidence rate was 93.55%...

Family farm sustainability is an essential guarantee for increasing the resilience of food systems. Based on network embeddedness theory and entrepreneurial bricolage theory, exploratory longitudinal case study was adopted. The value-creating behaviors family farms in different periods were described a process model with constructed. revealed that faced resource constraints such as shortage element resources, insufficient market resources lack knowledge during induction, start-up, growth...

Certain plant genotypes can achieve optimal growth under appropriate environmental conditions. Under high planting density conditions, plants undergo competition for uptake and utilization of light nutrients. However, the relationship between whole-genome expression patterns in perennial woody remains unknown. In this study, RNA sequencing poplar (Populus × euramericana) was carried out at three different sampling heights to determine gene (HD) low (LD) densities. As a result, 4,004...

Circular RNAs (CircRNAs) regulate plant growth and development; however, their role in poplar heterosis is unclear. We identified 3722 circRNAs leaves, most of which were intergenic (57.2%) exonic (40.2%). The expression F1 hybrids with high potential was higher than that those low potential. Non-additive single-parent (SPE-circRNAs) might through microRNA sponging protein translation, respectively. DECs among different potentials the via sponging. Correlation analysis between circRNA its...

Salt stress is one of the main factors that affect both growth and development plants. Maintaining K+/Na+ balance in cytoplasm important for metabolism as well salt resistance In present study, we monitored (height diameter) transgenic Populus alba × P. berolinensis trees (ABJ01) carrying JERF36s gene (a tomato jasmonic/ethylene responsive gene) over 4 years, which showed faster significant tolerance compared with non-transgenic poplar (9#). The expression NHX1 SOS1 genes encode Na+/H+...

ABSTRACT What is the effect of phyllosphere microorganisms on litter decomposition in absence colonization by soil microorganisms? Here, we simulated standing stage field to study differences composition and structure microbial community after mixed Populus × canadensis Pinus sylvestris var. mongolica litter. After 15 months decomposition, discovered that litters were not contact with had an antagonistic (the actual rate was 18.18%, which lower than expected rate) difference between...

Poplar, a model for woody plant research, is the most widely distributed tree species in world. Metabolites are basis of phenotypes, allowing an intuitive and effective understanding biological processes their mechanisms. However, metabolites non-transgenic multi-gene transgenic poplar remains poorly characterized, especially regards influences on quantity analysis relative abundance after introduction multi stress-related genes. In this study, we investigated cambium metabolomes one (D5-0)...

Insect infestations cause substantial changes in the leaves they attack, but effects of insect infestation on photosynthesis and gene expression whole period are rarely reported. In this study, poplar aphid (Chaitophorus populialbae Boyer de Fonscoloube) photosynthetic rate Populus alba var. pyramidalis were studied, from 2 to 36 days after low-density inoculation (DAI). The results revealed a dynamic change infestation: compensatory increased at 8 DAI, was inhibited 17 repaired 21 DAI....