A5067692129- Skin and Cellular Biology Research
- Cardiovascular Effects of Exercise
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Sports injuries and prevention
- Glycosylation and Glycoproteins Research
- Plant Reproductive Biology
- Cardiomyopathy and Myosin Studies
- Pharmacological Effects of Natural Compounds
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Autoimmune Bullous Skin Diseases
- Microbial Metabolism and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Spaceflight effects on biology
- Polyamine Metabolism and Applications
- Urticaria and Related Conditions
- Gut microbiota and health
- Cardiac electrophysiology and arrhythmias
University of Basel
2019-2024
Ludwig-Maximilians-Universität München
2017-2020
Friedrich-Alexander-Universität Erlangen-Nürnberg
2015
Arrhythmogenic cardiomyopathy (ACM) is characterized by progressive loss of cardiomyocytes with fibrofatty tissue replacement, systolic dysfunction, and life-threatening arrhythmias. A substantial proportion ACM caused mutations in genes the desmosomal cell-cell adhesion complex, but underlying mechanisms are not well understood. In current study, we investigated relevance defective for development progression.We mutated binding site DSG2 (desmoglein-2), a crucial molecule cardiomyocytes....
Glycosylation is essential to facilitate cell–cell adhesion and differentiation. We determined the role of dolichol phosphate mannosyltransferase (DPM) complex, a central regulator for glycosylation, desmosomal adhesive function epidermal Deletion key molecule DPM DPM1, in human keratinocytes resulted weakened adhesion, impaired localization components desmoplakin desmoglein-2, led cytoskeletal organization defects keratinocytes. In 3D organotypic epidermis model, loss DPM1 caused...
Pemphigus is an autoimmune dermatosis in which mucocutaneous blisters are induced primarily by autoantibodies against Desmoglein (Dsg) 1 and 3. vulgaris (PV) usually associated with Dsg3 whereas pemphigus foliaceus (PF) patients present Dsg1. Several signaling pathways were proposed to cause loss of keratinocyte adhesion. However, relevance different role Dsg1 3 trigger not fully understood. Here, we show that Ca2+ chelation reduced PV-IgG- PF-IgG-mediated HaCaT cohesion EGFR inhibition did...
Caenorhabditis elegans is one of the major model systems in biology based on advantageous properties such as short life span, transparency, genetic tractability and ease culture using an Escherichia coli diet. In its natural habitat, compost rotting plant material, this nematode lives bacteria. However, C. a predator bacteria, but can also be infected by nematopathogenic coryneform bacteria Microbacterium Leucobacter species, which display intriguing diverse modes pathogenicity. Depending...
Desmoplakin (Dp) is a crucial component of the desmosome, supramolecular cell junction complex anchoring intermediate filaments. The mechanisms how Dp modulates cell-cell adhesion are only partially understood. Here, we studied impact on function desmosomal molecules, desmosome turnover and intercellular adhesion.CRISPR/Cas9 was used for gene editing human keratinocytes which were characterized by Western blot immunostaining. Desmosomal ultrastructure assessed electron microscopy assays....
ABSTRACT Desmoplakin (Dp) localizes to desmosomes, linking clusters of desmosomal adhesion molecules the intermediate filament cytoskeleton. Here, we generated Dp knockout (ko) cell lines human keratinocytes study impact on and desmosome turnover using atomic force microscopy superresolution imaging. In comparison ko another component, plakoglobin (Pg), loss resulted in absence desmosomes drastically impaired cohesion. ko, desmoglein 2 (Dsg2) desmocollin 3 (Dsc3) were redistributed into...
Desmosomes are cell‐cell junctions which provide strong intercellular adhesion and couple the intermediate filament cytoskeleton to sites of contact. We have previously shown that actin‐binding protein α‐adducin is required for desmosome‐mediated adhesion. here tested in more detail mechanisms underlying α‐adducin‐dependent regulation desmosomal turnover Experiments were conducted human keratinocytes with siRNA‐mediated knock‐down α‐adducin, knock‐out cell lines obtained CRISPR/Cas9 system...
ABSTRACT Glycosylation is essential to facilitate cell-cell adhesion and differentiation. We determined the role of dolichol phosphate mannosyltransferase (DPM) complex, a central regulator for glycosylation, desmosomal adhesive function epidermal Deletion key molecule DPM DPM1, in human keratinocytes resulted weakened adhesion, impaired localization components desmoplakin desmoglein-2, led cytoskeletal organization defects keratinocytes. In 3D organotypic epidermis model, loss DPM1 caused...
Abstract Background Arrhythmogenic Cardiomyopathy (ACM) is characterized by progressive loss of cardiomyocytes with fibrofatty replacement, systolic dysfunction and life-threatening arrhythmias. A substantial proportion ACM caused mutations in genes the desmosomal cell-cell adhesion complex, but underlying mechanisms are not well understood. So far, treatment options only symptomatic. Here, we investigate relevance defective for development progression. Methods We mutated binding site...