A5002497695- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Biopolymer Synthesis and Applications
- Enzyme Catalysis and Immobilization
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
Jiangnan University
2019-2022
To establish the biotechnology platforms for production of bio‐based chemicals in various micro‐organisms is considered as a promising target to improve renewable isoprene. In this study, we heterologously expressed mevalonate (MVA) isoprene biosynthesis pathway, and explored three strategies increasing Escherichia coli. We first manipulated expression levels MVA pathway genes through changing gene cassettes promoters. introduce cofactor engineering, then overexpressed NADP‐dependent...
Co-localizing biochemical processes is a great strategy when expressing the heterologous metabolic pathway for product biosynthesis. The RNA scaffold flexible and efficient synthetic compartmentalization method to co-localize enzymes involved in by binding specific RNA, domains fused with engineered enzymes. Herein, we designed two artificial structures─0D scaffolds 2D scaffolds─using reported aptamers PP7 BIV-Tat corresponding RNA-binding (RBDs). We verified interaction of RBD aptamer vitro...
Poly-γ-glutamic acid (γ-PGA) is an excellent water-soluble biosynthesis material. To confirm the rate-limiting steps of γ-PGA pathway, we introduced a heterologous Bacillus strain pathway and employed enzyme-modulated dismemberment strategy in Escherichia coli.In this study, heterologously two laboratory-preserved strains-Bacillus amyloliquefaciens FZB42 subtilis 168 into E. coli, compared their production levels. Next, by changing plasmid copy numbers supplying sodium glutamate, explored...