A5025431883- Microbial Metabolic Engineering and Bioproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Protein purification and stability
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Enzyme Production and Characterization
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Catalysis and Immobilization
- Animal Virus Infections Studies
- Advanced biosensing and bioanalysis techniques
- Herpesvirus Infections and Treatments
- Microbial Natural Products and Biosynthesis
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- Algal biology and biofuel production
- Fungal Biology and Applications
- Animal Disease Management and Epidemiology
- Environmental Toxicology and Ecotoxicology
- Fermentation and Sensory Analysis
- Biotin and Related Studies
- Bacterial Genetics and Biotechnology
Jiangnan University
2016-2025
Zhengzhou University
2025
Henan University
2022
SciTech Development (United States)
2013
GlaxoSmithKline (United Kingdom)
2010
Age UK
2010
Johnson & Johnson (United Kingdom)
2007
Imperial College London
2007
Fraunhofer Institute for Toxicology and Experimental Medicine
2005
University of Strathclyde
2001-2003
Asthma occurs accompanied by the ferroptosis in bronchial epithelial cells, during which Interleukin-6 (IL-6) plays a key role. However, associations between IL-6, and asthma have not been reported. Bronchial cells BEAS-2B were induced different concentrations of IL-6 cell viability was detected MTT assay. The TBARS production rate corresponding kit. expression oxidative stress-related indexes ELISA. Iron Assay Kits total iron levels ferrous ion (Fe2+) levels. Labile pool assay used to...
Lethal necrotizing fasciitis caused by Streptococcus pyogenes is characterized a paucity of neutrophils at the site infection. Interleukin (IL)–8, which important for neutrophil transmigration and activation, can be degraded S. pyogenes. Blood isolates were better able to degrade human IL-8 than throat isolates. Degradation was result single specific cleavage between 59glutamine 60arginine within C-terminal α helix. Cleaved reduced activation migration. IL-8–cleaving activity found in...
Thousands of medical staff have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), hundreds deaths reported. Such loss could be prevented if there were a serologic assay for SARS-CoV-2-specific antibodies serological surveillance its infection at the early stage disease.Using Chinese hamster ovarian (CHO) cell-expressed full-length SARS-CoV-2 S1 protein as capturing antigen, disease 2019 (COVID-19)/SARS-CoV-2 serology enzyme-linked immunosorbent (ELISA) kit was...
Corynebacterium glutamicum (C. glutamicum) has traditionally been used as a microbial cell factory for the industrial production of many amino acids and other industrially important commodities. C. recently established host recombinant protein expression; however, some intrinsic disadvantages could be improved by genetic modification. Gene editing techniques, such deletion, insertion, or replacement, are tools modifying chromosomes. In this research, we report CRISPR/Cas9 system in rapid...
Exploring the molecular difference among breast cancer subtypes is of crucial importance in understanding its heterogeneity and seeking effective clinical treatment. For this, several layers information including immunohistochemical markers a variety high-throughput genomics approaches have been intensively used. Here we explored intrinsic differences subgroups defined by expression (IHC) hormone receptors ER PR as well human epidermal growth factor receptor 2 (HER2) using mRNA miRNA...
Abstract β‐elemene is one of the most commonly used antineoplastic drugs in cancer treatment. As a plant‐derived natural chemical, biologically engineering microorganisms to produce germacrene A be converted harbors great expectations since chemical synthesis and plant isolation methods come with their production deficiencies. In this study, we report design an Escherichia coli cell factory for de novo from simple carbon source. series systematic approaches isoprenoid central pathways,...
This research study presents a precise, accurate, and linear liquid-chromatography method for the determination of pemafibrate in tablet dosage forms, despite presence high volume excipients. Various challenges were addressed through experimental approaches evidence-based solutions. The employs suitable stationary phase, X-Bridge C18 (150 × 4.6 mm, 3.5 μm), an appropriate isocratic program. Key parameters include flow rate 1.0 mL/min, column temperature 40°C, injection 10 μL, runtime 15 min....
Polyketides are a class of natural products with many applications but mainly appealing as pharmaceuticals. Heterologous production polyketides in the yeast Saccharomyces cerevisiae has been widely explored because merits this model eukaryotic microorganism. Although acetyl-CoA and malonyl-CoA, precursors for polyketide synthesis, distributed several subcellular organelles, only cytosolic synthesis pursued previous studies. In study, we investigate by directly using peroxisomes strain...