Magnesium (Mg) is an essential mineral element for plants and nontoxic to organisms. In this study, we took advantage of nanotechnologies systematically investigate the antibacterial mechanisms magnesium oxide nanoparticles (MgONPs) against phytopathogen Ralstonia solanacearum (R. solanacearum) in vitro vivo first time. R. has contributed catastrophic bacterial wilt, which resulted world-wide reduction tobacco production. The results demonstrated that MgONPs possessed statistically...

Engineered nanoparticles have provided a basis for innovative agricultural applications, specifically in plant disease management. In this interdisciplinary study, by conducting comparison studies using macroscale magnesium oxide (mMgO), we evaluated the fungicidal activity of MgO (nMgO) against soilborne Phytophthora nicotianae and Thielaviopsis basicola first time under laboratory greenhouse conditions. vitro revealed that nMgO could inhibit fungal growth spore germination impede...

The substantial antimicrobial efficacy of nanoparticles against phytopathogens has been extensively investigated for advanced agricultural applications. However, few reports have focused on soilborne pathogenic bacteria. aim this study was to obtain sustainably synthesized copper oxide (CuONPs) using papaya leaf extracts and investigate the bactericidal activity these CuONPs Ralstonia solanacearum, cause bacterial wilt, under laboratory greenhouse conditions. results showed that possessed...

Coumarins are important plant-derived natural products with wide-ranging bioactivities and extensive applications. In this study, we evaluated for the first time antibacterial activity mechanisms of action coumarins against phytopathogen Ralstonia solanacearum, investigated effect functional group substitution. We tested 18 different substitution patterns, found that daphnetin, esculetin, xanthotol, umbelliferone significantly inhibited growth R. solanacearum. Daphnetin showed strongest...

Soil acidification is a major problem in modern agricultural systems and an important factor affecting the soil microbial community health. However, little known about effect of on soil-borne plant diseases. We performed 4-year investigation South China to evaluate correlation between occurrence bacterial wilt. The results showed that average pH fields infected by wilt disease was much lower than non-disease fields. Moreover, proportion soils with 5.5 higher non-infected soils, this...

Although bacterial communities play important roles in the suppression of pathogenic diseases and crop production, little is known about associated with wilt. Based on 16S rRNA gene sequencing, statistical analyses microbial disease-suppressive disease-conducive soils from three districts during vegetation period tobacco showed that Proteobacteria was dominant phylum, followed by Acidobacteria. Only samples September were significantly correlated to disease factors. Fifteen indicators taxa...

Application of soil amendments has been wildly used to increase pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community crops, especially when field naturally harbors disease wilt tobacco for many years due long-term continuous cropping acidification. In this study, biochar (CP), lime (LM), oyster shell powder (OS) no amendment additions (Control; CK) were assessed their abilities improve acidification, change composition communities...

In this study, we documented the impact of magnesium oxide nanoparticles (MgONPs) on various morpho-physiological changes by root irrigation in tobacco plants matrix media, as well uptake and accumulation NPs over a range concentrations (50⁻250 μg/mL). Our results showed that seed germination rate was not affected following exposure to MgONPs for 5 days. Enhanced plant growth together with increased peroxidase activity (39.63 U mg-1 protein 250 μg/mL treatment, 36.63 control), superoxide...

In this paper, the enhanced antibacterial activity of silver nanoparticles (AgNPs) against phytopathogenic bacterium Ralstonia solanacearum after stabilization using selected surfactants (SDS, SDBS, TX-100, and Tween 80) was examined, in comparison with ion. 80 found to be most preferable stabilizer AgNPs due beneficial synergistic effects surfactant. However, all nearly had no on Ag + . vitro , 80-stabilized showed highest bactericidal R. Further measurements TEM, fluorescence microscopy,...

Protocatechualdehyde (PCA) is an important plant-derived natural product that has been associated with a wide variety of biological activities and widely used in medicine as antioxidant, anti-aging anti-inflammatory agent. However, fewer reports concerning its antibacterial effects on plant-pathogenic bacteria exist. Therefore, this study, protocatechualdehyde was evaluated for activity against plant pathogens along the mechanism action. PCA at 40 μg/mL highly active R. solanacearum...

Investigations into the potential application of nanoparticles acting as nanofungicides in sustainable agriculture are rapidly expanding due to high antimicrobial properties these compounds, which do not risk inducing pathogen resistance fungicides. A detailed understanding impact copper oxide (CuO NPs) on soil-borne phytopathogenic fungi is yet be obtained. This study aimed explore vitro antifungal activity and control efficacy CuO NPs applied via irrigation with respect tobacco black shank...

Bacterial wilt is accompanied by microbial communities shift and soil acidification. However, the relationship between changes of bacterial under influence different acidification levels has not been fully elucidated. Here, we analyzed abundance Ralstonia solanacearum, rhizosphere carbon metabolism at differently acidic (pH 6.45, pH 5.60, 5.35, 4.90 4.45) amendment treatment (CaO). The results indicated that both R. solanacearum incidence showed a significant trend first increasing then...

An increasing number of investigations have been performed on the phytotoxicity carbon-based nanomaterials duo to their extensive use in various fields. In present study, we investigated unfunctionalized graphene oxide (GO) and amine-functionalized (G-NH₂) wheat (Triticum aestivum) concentration range from 125 2000 μg/mL after 9 days hydroponic culture. Our results found that incubation with both did not affect final seed germination rate, despite some influence initial stage. Transmission...

WRKY transcription factors (TFs) modulate plant responses to biotic and abiotic stresses. Here, we characterized a IIc TF, NtWRKY50, isolated from tobacco (Nicotiana tabacum) plants. The results showed that NtWRKY50 is nuclear-localized protein its gene transcript induced in when inoculated with the pathogenic bacterium Ralstonia solanacearum. Overexpression of enhanced bacterial resistance, which correlated SA JA/ET signaling genes. However, silencing had no obvious effects on disease...

Bacterial wilt is a destructive disease caused by the phytopathogen Ralstonia solanacearum (R. solanacearum), which widely found in various tobacco-growing areas all over world. Botanical bactericidal substances have gradually emerged as hot topic modern pesticide research. In this study, antibacterial activities of two phytochemicals (resveratrol and coumarin) against R. their vivo vitro efficacy for controlling tobacco bacterial were evaluated. We rule out significant biological effects...

Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease resulting in tremendous losses of economic crops such as plants the Solanaceae. Recent studies showed that R. solanacearum spreading from lowlands to highlands China. We studied 97 Chinese strains were isolated four tobacco-growing zones over wide range elevations using phylotype specific multiplex polymerase chain reaction (Pmx-PCR) and phylogenetic relationships (egl mutS). The results all isolates belonged I, which...

In rhizospheres, chemical barrier-forming natural compounds play a key role in preventing pathogenic bacteria from infecting plant roots. Here, we sought to identify specific phenolic exudates tobacco ( Nicotiana tobaccum ) plants infected by the soil-borne pathogen Ralstonia solanacearum that may exhibit antibacterial activity and promote resistance against pathogens. Among detected acids, only caffeic acid was significantly induced R. relative healthy plants, concentration of reached 1.95...

Introduction Native endophytic microorganisms in tobacco seeds are closely related to their resistance Ralstonia solanacearum ( R. ) infections. However, the role of native seed core microbiome suppression bacterial wilt disease (BWD) remains underexplored. Methods The characteristics communities both resistant and susceptible varieties were characterized using high-throughput sequencing technology. Results This study found Paenibacillus as a potential microbial antagonist against BWD based...

ABSTRACT The root-knot nematode (RKN) Meloidogyne incognita is one of the most destructive plant-parasitic nematodes (PPNs) affecting crop production worldwide. Our earlier study revealed that calcinated oyster shell powder (OSP) effectively suppressed tobacco RKN disease. However, suppression mechanism OSP against RKNs remain unknown. reduced root–knot index by more than 38% inhibiting migration second-stage juveniles (J2) in soil. Furthermore, J2 density rhizosphere 43.69% and...

After continuous cropping for many years, crops are often subject to growth inhibition, which seriously affects their yields.In agricultural production, soil fumigation can effectively alleviate the biological stress on plants. However, relationship between microbial groups that respond changes and plants, as well whether existence makes a beneficial contribution remains unclear. We explored mechanism of promoting plant by affecting microorganisms. The results showed dazomet treatment...

The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR 35 distinct amplimers aligning at a 91% similarity level, reflecting high degree genotypic among bacteria. A total 25 16S-RFLP patterns were identified representing over 33 species 17 different genera....