A5056204056- Respiratory viral infections research
- Influenza Virus Research Studies
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- Educational Reforms and Innovations
- Advanced biosensing and bioanalysis techniques
- HIV/AIDS Research and Interventions
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Tracheal and airway disorders
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Pneumonia and Respiratory Infections
- Artificial Intelligence Applications
- Educational Innovations and Challenges
- Biotechnology and Related Fields
- Hepatitis B Virus Studies
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- interferon and immune responses
- Viral Infections and Immunology Research
- Chinese history and philosophy
- Innovative Educational Techniques
Chinese Academy of Sciences
2015-2025
University of Chinese Academy of Sciences
2019-2025
Institut Pasteur of Shanghai
2015-2023
Xi'an University of Science and Technology
2023
SAIC-GM (China)
2019
Education Department of Hunan Province
2018-2019
Hunan University of Humanities, Science and Technology
2017-2019
China National Center for Biotechnology Development
2006-2017
Hunan University of Science and Technology
2009-2011
Second People's Hospital of Yunnan Province
2011
Human coronaviruses HCoV-OC43, HCoV-229E, HCoV-NL63 and HCoV-HKU1 are common respiratory viruses associated with acute infection. They have a global distribution. Rapid accurate diagnosis of HCoV infection is important for the management treatment hospitalized patients Here, we developed melting curve-based multiplex RT-qPCR assay simultaneous detection four HCoVs. In assay, SYTO 9 was used to replace SYBR Green I as fluorescent dye, GC-modified primers were designed improve temperature (Tm)...
Abstract HIV-1 epidemics among MSM are a major public health concern in China, especially large cities. This study sought to better understand the dynamics of HIV molecular epidemiology Shenzhen, rapidly developing city with over 13.8 million people. pol sequences were obtained from 996 (53.5%) 1862 HIV-infected and 403(9.0%) 4498 heterosexuals injection drug users China 2005-2012. Eight subtypes some inter-subtype recombinants identified sampled CRF07_BC (39.1%) CRF01_AE (35.1%) being most...
Abstract Recurrent acute respiratory tract infections (ARTIs) affect a large population, yet the specific decisive factors are largely unknown. Here we study population of 4407 children diagnosed with ARTI, comparing virome and serum cytokine profiles associated multiple ARTIs single ARTI during six-year period. The relative abundance Propionibacterium phages is significantly elevated in compared to group. Serum levels TIMP-1 PDGF-BB markedly increased single-ARTI non-ARTI controls, making...
Loop-mediated isothermal amplification (LAMP) has been widely used in the detection of pathogens causing infectious diseases. However, mismatches between primers (especially 3'-end) and templates significantly reduced efficiency LAMP, limited its application to genetically diverse viruses. Here, we reported a novel mismatch-tolerant LAMP assay dengue viruses (DENV). The method features addition as little 0.15 U high-fidelity DNA polymerase standard 25 μl reaction mixture. This amount was...
Financial access to HIV care and treatment can be difficult for many people in China, where the government provides free antiretroviral drugs but does not cover cost of other medically necessary components, such as lab tests opportunistic infections. This article estimates out-of-pocket costs that a person living with HIV/AIDS China might face over course one year. Data comes from two projects run by Médecins Sans Frontières Nanning, Guangxi Province Xiangfan, Hubei Province. Based on...
Quantitative PCR (qPCR) is widely used to detect viruses. However, mismatches occurring in the 3′-end of primers reduce amplification efficiency qPCR and limit its capacity detection highly variable Here, we reported a mismatch-tolerant RT-qPCR with small amount additional high-fidelity DNA polymerase for simultaneous RSV-A RSV-B. The novel assay had higher various variants forming than conventional RT-qPCR, showed good specificity sensitivity. It demonstrated correlation coefficient...
The on-going generation of HIV-1 intersubtype recombination has led to new circulating recombinant forms (CRFs) and unique (URFs) in Asia. In this study, we evaluated whether previously reported URFs were actually CRFs. All available complete or near full-length URF sequences from Asia retrieved the HIV Los Alamos National Laboratory Sequence database, phylogenetic, transmission cluster, bootscan analyses performed using MEGA 6.0, Cluster Picker 1.2.1, SimPlot3.5.1. According criterion CRFs,...
Polymerase chain reaction (PCR) has extensive bioanalytical applications in molecular diagnostics and genomic research studies for rapid detection precise amplification. Routine integrations analytical workflow indicate certain limitations, including low specificity, efficiency, sensitivity conventional PCR, particularly towards amplifying high guanine-cytosine (GC) content. Further, there are many ways to enhance the reaction, example, using different PCR strategies such as...
Influenza A virus (IAV) causes highly contagious respiratory disease worldwide, so prevention and control of IAV is extremely important. However, overuse neuraminidase inhibitor (NAI) drugs leads to drug resistance. To explore the up-to-date geographical distribution evolution drug-resistant mutations (DRMs) in NA protein IAV, 81,492 near full-length sequences downloaded from NCBI GISAID databases, including 34,481 H1N1 46,622 H3N2, were processed analyzed. Our results showed annual number...
A simple method, primer specific and mispair extension analysis (PSMEA) with pfu DNA polymerase was developed for genotyping. PSMEA is based on the unique properties of 3'-->5' exonuclease proofreading activity. In presence an incomplete set dNTPs, found to be extremely discriminative in nucleotide incorporation at initiation step synthesis, completely preventing when mispair(s) are adjacent 3'-end primer. This has allowed us accurately detect variations, deletions insertions fast
The biggest challenge for accurate diagnosis of viral infectious disease is the high genetic variability involved viruses, which affects amplification efficiency and results in low sensitivity narrow spectrum. Here, we developed a new simple qPCR mediated by high-fidelity (HF) DNA polymerase. method utilizes an HFman probe one primer. Fluorescent signal was generated from 3'-5' hydrolysis HF polymerase before elongation initiation. Mismatches between probe/primer template have less influence...
Influenza viruses are respiratory pathogens that major threats to human health. Due the emergence of drug-resistant strains, use traditional anti-influenza drugs has been hindered. Therefore, development new antiviral is critical. In this article, AgBiS2 nanoparticles were synthesized at room temperature, using bimetallic properties material itself explore its inhibitory effect on influenza virus. By comparing Bi2S3 and Ag2S nanoparticles, it found after adding silver element, have a...
Introduction: Influenza is a severe respiratory viral infection that causes significant morbidity and mortality, due to annual epidemics unpredictable pandemics. With the extensive use of neuraminidase inhibitor (NAI) drugs, influenza B virus has carried different drug-resistant mutations. Thus, this study aimed analyze prevalence mutations virus. Methodology: Near full-length sequences (NA) region all viruses from January 1, 2006, December 31, 2018, were downloaded public databases GISAID...
Introduction: Human respiratory syncytial virus (hRSV) is a common closely related to tract infection (RTI). Rapid and accurate detection of hRSV urgently needed reduce the high morbidity mortality due infection.
 Methodology: Here, we established highly sensitive specific reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid A B group simultaneously. The primer sets groups were designed in M M2-2 gene, respectively. SYTO 9 was used as fluorescent dye...