A5078949545- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- Neuroendocrine regulation and behavior
University of Toronto
2023-2024
Cell migration is crucial for development and tissue homeostasis, while its dysregulation leads to severe pathologies. driven by the extension of actin–based lamellipodia protrusions, powered actin polymerization, which tightly regulated signaling pathways, including Rho GTPases Ca 2+ signaling. While importance in protrusions has been established, molecular mechanisms linking assembly are unknown. Here, we identify a novel axis involving mechano-gated channel TRPV4, regulates various cell...
The nematode Caenorhabditis elegans is widely employed as a model organism to study basic biological mechanisms. However, transgenic C. are generated by manual injection, which remains low-throughput and labor-intensive, limiting the scope of approaches benefitting from large-scale transgenesis. Here, we report robotic microinjection system, integrating microfluidic device capable reliable worm immobilization, transfer, rotation, for high-speed injection elegans. system provides an speed 2-3...