A5026788398- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Digital Imaging for Blood Diseases
- Cell Image Analysis Techniques
- Neurobiology and Insect Physiology Research
- Olfactory and Sensory Function Studies
- Photoreceptor and optogenetics research
- Spaceflight effects on biology
- AI in cancer detection
- Medical Image Segmentation Techniques
- Neuroendocrine regulation and behavior
- Memory and Neural Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Memory Processes and Influences
- Neuroinflammation and Neurodegeneration Mechanisms
- Light effects on plants
- Brain Tumor Detection and Classification
- Single-cell and spatial transcriptomics
- Microtubule and mitosis dynamics
- Sleep and Wakefulness Research
Harvard University
2017-2025
Harvard University Press
2023-2025
Hunan University
2024
Georgia Institute of Technology
2023
Beijing Normal University
2023
Center for Pain and the Brain
2023
Huazhong University of Science and Technology
2015-2020
Evolutionary Genomics (United States)
2018-2019
Institute of Biophysics
2016
Sensory modulation is essential for animal sensations, behaviours and survival. Peripheral modulations of nociceptive sensations aversive are poorly understood. Here we identify a biased cross-inhibitory neural circuit between ASH ASI sensory neurons. This inhibition to drive normal adaptive avoidance CuSO4 (Cu(2+)) challenge in Caenorhabditis elegans. In the circuit, ASHs respond Cu(2+) robustly suppress ASIs via electro-synaptically exciting octopaminergic RIC interneurons, which release...
Forgetting is defined as a time-dependent decline of memory. However, it not clear whether forgetting reverses the learning process to return brain naive state. Here, using aversive olfactory pathogenic bacteria in C. elegans , we show that generates novel state nervous system distinct from or learned A transient exposure training condition odorants reactivates this elicit previously behavior. An AMPA receptor and type II serotonin act central neuron circuit decrease increase speed reach...
Cell identification is an important yet difficult process in data analysis of biological images. Previously, we developed automated cell method called CRF_ID and demonstrated its high performance Caenorhabditis elegans whole-brain images (Chaudhary et al., 2021). However, because the was optimized for imaging, comparable could not be guaranteed application commonly used C. multi-cell that display a subpopulation cells. Here, present advancement, 2.0, expands generalizability to imaging...
Abstract Modulation of gap junction-mediated electrical synapses is a common form neural plasticity. However, the behavioral consequence modulation and underlying molecular cellular mechanisms are not understood. Here, using C. elegans circuit interneurons that connected by junctions, we show junctions facilitates olfactory learning. Learning experience weakens induces repulsive sensory response to training odorants, which together decouple responses odorants generate learned behavior. The...
Olfactory integration is important for survival in a natural habitat. However, how the nervous system processes signals of two odorants present simultaneously to generate coherent behavioral response poorly understood. Here, we characterize circuit basis form olfactory Caenorhabditis elegans . We find that presence repulsive odorant, 2-nonanone, threat strongly blocks attraction other odorants, such as isoamyl alcohol (IAA) or benzaldehyde, signal food. Using forward genetic screen, found...
In the natural environment, animals often encounter multiple sensory cues that are simultaneously present. The nervous system integrates relevant information to generate behavioral responses have adaptive values. However, neuronal basis and modulators regulate integrated response not well defined. Here, we address this question using a decision in C. elegans when animal is presented with an attractive food source together repulsive odorant. We identify specific neurons, interneurons...
Animals have developed the ability to sense water content in their habitats, including hygrosensation (sensing humidity air) and hydrosensation other microenvironments), they display preferences for specific contents that influence mating, reproduction geographic distribution. We employed four quantitative behavioural test paradigms investigate molecular cellular mechanisms underlying sensing an agar substrate (hydrosensation) hydrotaxis Caenorhabditis elegans. By combining a reverse genetic...
Pentameric ligand-gated ion channels (LGICs) play conserved, critical roles in both excitatory and inhibitory synaptic transmission can be activated by diverse neurochemical ligands. We have performed a characterization of orphan from the nematode C. elegans, identifying five new monoamine-gated LGICs with functional properties expression postsynaptic to aminergic neurons. These include polymodal anion dopamine tyramine, which may mediate molecules vivo. Intriguingly, we also find that novel...
Specific recording, labeling, and spatiotemporal manipulating neurons are essential for neuroscience research. In this study, we developed a tripartite gene induction system in C. elegans, which is based on the knockout of two transcriptional terminators (stops short) by different recombinases FLP CRE. The recombinase sites (loxP FRT) flanked stops after ubiquitous promoter terminate transcription target genes. CRE, induced promoters overlapping expression, remove (subsequent FLP/CRE-out)....
Cell identification is an important yet difficult process in data analysis of biological images. Previously, we developed automated cell method called CRF_ID and demonstrated its high performance C. elegans whole-brain images (Chaudhary et al, 2021). However, because the was optimized for imaging, comparable could not be guaranteed application commonly used multi-cell that display a subpopulation cells. Here, present advance 2.0 expands generalizability to imaging beyond imaging. To...
Cell identification is an important yet difficult process in data analysis of biological images. Previously, we developed automated cell method called CRF_ID and demonstrated its high performance C. elegans whole-brain images (Chaudhary et al, 2021). However, because the was optimized for imaging, comparable could not be guaranteed application commonly used multi-cell that display a subpopulation cells. Here, present advance 2.0 expands generalizability to imaging beyond imaging. To...
<title>Abstract</title> Learning and memory are essential for animals’ well-being surviving. The underlying mechanisms a major task of neuroscience studies. In this study, we identified circuit consisting ASER, RIC, RIS, AIY, required short-term salt chemotaxis learning (SCL) in <italic>C. elegans</italic>. ASER NaCl sensation possesses ON- OFF-responses is remodeled by conditioning. RIC integrates sensory information stimulation food deprivation generates suppression its calcium response...
Cell identification is an important yet difficult process in data analysis of biological images. Previously, we developed automated cell method called CRF_ID and demonstrated its high performance C. elegans whole-brain images (Chaudhary et al, 2021). However, because the was optimized for imaging, comparable could not be guaranteed application commonly used multi-cell that display a subpopulation cells. Here, present advance 2.0 expands generalizability to imaging beyond imaging. To...
Summary Pentameric ligand-gated ion channels (LGCs) play conserved, critical roles in fast synaptic transmission, and changes LGC expression localisation are thought to underlie many forms of learning memory. The C. elegans genome encodes a large number LGCs without known ligand or function. Here, we deorphanize five members family Cys-loop by characterizing their diverse functional properties that activated biogenic amine neurotransmitters. To analyse the neuronal function these LGCs, show...
ABSTRACT Olfactory masking is a complex olfactory response found in humans. However, the mechanisms whereby presence of one odorant masks sensory and behavioral responses elicited by another are poorly understood. Here, we report that Caenorhabditis elegans displays repulsive odorant, 2-nonanone, signals threat strongly attraction other odorants, such as isoamyl alcohol (IAA) or benzaldehyde food. Using forward genetic screen, several genes, osm-5, osm-1 , dyf-7 known to regulate structure...
Abstract Cell identification is an important yet difficult process in data analysis of biological images. Previously, we developed automated cell method called CRF_ID and demonstrated its high performance C. elegans whole-brain images (Chaudhary et al, 2021). However, because the was optimized for imaging, comparable could not be guaranteed application commonly used multi-cell that display a subpopulation cells. Here, present advance 2.0 expands generalizability to imaging beyond imaging. To...
Cell identification is an important yet difficult process in data analysis of biological images. Previously, we developed automated cell method called CRF_ID and demonstrated its high performance Caenorhabditis elegans whole-brain images (Chaudhary et al., 2021). However, because the was optimized for imaging, comparable could not be guaranteed application commonly used C. multi-cell that display a subpopulation cells. Here, present advancement, 2.0, expands generalizability to imaging...
Abstract In the natural environment, animals often encounter multiple sensory cues that are simultaneously present. The nervous system integrates relevant information to generate behavioral responses have adaptive values. However, signal transduction pathways and molecules regulate integrated response not well defined. Here, we characterize a collective modulatory basis for decision in C. elegans when animal is presented with an attractive food source together repulsive odorant. We show...