Chamomile (Chamaemelum nobile) is widely used throughout the world, and has anti-inflammatory, deodorant, bacteriostatic, antimicrobial, carminative, sedative, antiseptic, anti-catarrhal, spasmolytic properties. Because of increasing incidence drug-resistant bacteria, development natural antibacterial sources such as medical herbs for treatment infectious diseases necessary. Extracts from different plant parts leaves, flowers, fruit, bark Combretum albiflorum, Laurus nobilis , Sonchus...

The emergence and dissemination of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates their involvement in several nosocomial outbreaks are high concern. This study was conducted to investigate the genetic relatedness molecular determinants carbapenem resistance 100 CRKP isolates. Susceptibility carbapenems as well other antibiotics determined by using disk diffusion method. Modified Hodge test performed for detection carbapenemase production. minimum inhibitory concentrations...

Accurate and timely detection of drug resistance can minimize the risk further development lead to effective treatment. The aim this study was determine first/second-line anti-tuberculosis drugs in rifampicin/multidrug-resistant Mycobacterium tuberculosis (RR/MDR-MTB) isolates. Molecular epidemiology strains determined using whole genome sequencing (WGS)-based genotyping. A total 35 RR/MDR-MTB isolates were subjected susceptibility testing against 7H9 Middlebrook broth microdilution method....

Drug resistance among Mycobacterium tuberculosis (MTB) strains is a growing concern in developing countries. We conducted comprehensive search for relevant studies Iran on PubMed, Scopus, and Embase until June 12, 2020. Our study focused determining the prevalence of antibiotic MTB isolates, with subgroup analyses based year, location, drug susceptibility testing (DST) methods. Statistical were performed using STATA software. meta-analysis included total 47 articles. Among new TB cases, we...

Microbial resistance to antibiotics and their adverse effects related these are a matter of global public health in the 21th century. The emergence drug-resistant strains, has gained interest scientists discover new antimicrobial agents from essential oil medicinal plants.Anti-mycobacterial Trachyspermum copticum Pelargonium graveolens oils were determined against multi-drug resistant clinical strains Mycobacterium tuberculosis, kansasii, fortuitum standard strain tuberculosis H37Rv by Broth...

Successful treatment of tuberculosis depends on early diagnosis and use appropriate drug susceptibility testing in a timely manner. In the present study, LPA efficacy was assayed detection pulmonary comparison to available methods Iran phylogenetic analyses isolated cases carried out by MIRU-VNTR.This study conducted at Tehran Regional Reference Laboratory for Tuberculosis. All sputum specimens were subjected smear, culture, (DST), GeneXpert, LPA. Finally, 15-locus-based MIRU-VNTR used...

The potentially pathogenic Non-Tuberculosis Mycobacteria (NTM) are emerging nowadays which result in pulmonary and non-pulmonary infections human. This group of bacteria consists at least 200 different species. While the disease is most common form NTM infections, can cause diffused as well extrapulmonary every organ, such bone marrow, skin, eye, brain. tuberculosis-like therefore, correct identification these necessary to avoid faulty treatment. Different species isolates were identified...

Here, we aimed to determine the susceptibility of 70 Mycobacterium tuberculosis isolates obtained from different regions country 8 anti-tuberculosis (anti-TB) drugs and possible underlying mechanisms causing resistance rifampin, isoniazid, pyrazinamide. The M. anti-TB was tested using proportion method. Strains showing first line were subjected PCR amplification sequencing rpoB, katG, ahpC, pncA genes, inhA promoter oxyR-ahpC intergenic detect conferring mutations. Overall, 77.1% resistant...

Background: Mycobacterium tuberculosis as an intracellular pathogen causes Tuberculosis (TB). Due to the long time required for treatment, hepatotoxicity of drugs and also emergence Multidrug-Resistant (MDR) Extremely Drug Resistant (XDR) strains, TB is currently a major public health problem. Some medicinal plants possess remarkable activity against Mycobacterium. Among them, Lamiaceae family are pharmaceutical interest because their potential antimicrobial properties. The aim study was...

Introduction: Mycobacterium tuberculosis (MTB), the causative agent of (TB), is a significant global public health threat. Besides extensive multidrug resistance, MTB possesses several properties for long-term viability in host as well stress adaptation and resistance harsh conditions. The role toxin-antitoxin (TA) systems disseminating maintaining antimicrobial bacterial populations has also been demonstrated. This study aimed to evaluate differences expression MazEF (a well-known TA...

Abstract Objective Staphylococcus aureus with the ability of biofilm formation and drug resistance acquisition is one most frequently isolated pathogens from chronic rhinosinusitis patients. Ultrasound as an alternative therapy effectively able to kill bacteria by cavitation in or on bacterial cells peroxide generation hence improving antibiotic treatment efficacy. Results was detected 4 6 out 14 patients phenotypic qPCR assays, respectively. Four were completely resolved after pulsed...

Background: Helicobacter pylori is the most common cause of chronic infection in human stomach. The has universe prevalence all age groups. Probably, this bacterium bacterial beings and infects approximately half world population. H. produces urease, an enzyme that degrades urea stomach’s mucous to ammonia resulting biochemical reaction leads increase pH stomach lumen. This allows pathogenic intestinal protozoa take opportunity cross through increased disease. aim study was evaluate...

Salmonella enterica serovar Typhimurium (S. Typhimurium) causes gastroenteritis in humans and paratyphoid disease some animals. Given the emergence of antibiotic resistance, vaccines are more effective than chemotherapy control.The aim this experimental study was to evaluate immunogenicity diphtheria toxoid (DT) conjugated with S. -derived OPS (O side chain isolation) mice determine its potential as a vaccine candidate against salmonellosis.Lipopolysaccharide (LPS) extracted from bacterial...

Background: Nocardia, Streptomyces and Rhodococcus are life threatening opportunistic pathogens under immunodeficiency conditions, particularly among patients infected with HIV. Rapid accurate detection of these infections can improve immune health quality, patient management appropriate treatment. The aim this study was to design a novel multiplex-PCR assay for rapid diagnosis three organisms directly from bronchoalveolar lavage (BAL) specimens HIV.Methods: genus specific primers were...

Nontuberculous mycobacterial (NTM) infections have radically increased worldwide due to the increase in HIV infections. The disease activity increases with progressive immunodeficiency.A total of 216 seropositive patients suspected having infection were recruited for this study. Clinical samples collected from each patient and cultured on Lowenstein-Jensen media. Detection species identification simultaneously done using Reverse Blot Hybridization Assay System. Also, minimum inhibitory...

Stenotrophomonas maltophilia isolates are responsible for various hospital‐acquired infections and particularly increasing in the immunocompromised patients. The aim of this study was to determine clonal relatedness between S. originating from clinic environment. A total 150 patients 1108 environmental samples obtained three hospitals Tehran. Following molecular identification targeting 23S rRNA gene, clinical determined using pulsed field gel electrophoresis (PFGE). Of 18 identified...