Human endogenous retroviruses (HERVs) constitute about 8% of the human genome. The overexpression HERVs has been detected in various inflammatory disorders like neuro-inflammation and cancer. Interestingly, it reported that stress conditions facilitate HERV expression. Space travel exposes astronauts to microgravity environments (a condition), which may result activation might influence pathogenic outcomes during after space flight. This study aimed elucidate transcriptional activity three...

Enterotoxigenic Bacteroides fragilis (ETBF) produces toxin (BFT), which is associated with acute diarrheal, inflammatory bowel disease, and colorectal cancer (CRC). In experimental models, ETBF has been shown to contribute colon carcinogenesis. The present study was conducted investigate mucosal colonization of in the find a possible association between presence precancerous cancerous lesions. biopsies involved sites were obtained from 68 patients lesions 52 healthy controls (HC). samples...

In this work, we observed an increased presence of antibodies (Abs) against type I interferon (IFN-I) in coronavirus disease 2019 (COVID-19) patients admitted to the intensive care unit (ICU) compared non-ICU COVID-19 and healthy control (HC) subjects. Human endogenous retrovirus W (HERV-W) can reactivate after viral infection; therefore, also investigated HERV-W envelope (HERV-W-env)-derived epitopes. A total 113 subjects (41 female 72 male subjects) were analyzed. significant difference...

Abstract Microgravity (µg) during spaceflight affects cellular and molecular functions of both human cells microbial pathogens, influencing viral replication the host immune system. This study aimed to investigate effects simulated µg on Herpes Simplex Virus-1 (HSV-1) replication, pro-inflammatory cytokine, endogenous retrovirus (HERV) activation in neuroblastoma SH-SY5Y cells. Our results show that has a negative impact HSV-1 leading significantly reduced titers lower expression levels...

Abstract Background Bacteroides fragilis is a part of the normal gastrointestinal flora, but it also most common anaerobic bacteria causing infection. It highly resistant to antibiotics and contains abundant antibiotic resistance mechanisms. Methods The pattern 78 isolates B. (22 strains from clinical samples 56 colorectal tissue) was investigated using agar dilution method. gene encoding fargilis toxin bft , genes were targeted by PCR assay. Results highest rate observed for penicillin G...

Alterations of the gut microbiome in cases colorectal cancer (CRC) hint at involvement host-microbe interactions onset and progression CRC also, possibly, provide novel ways to detect prevent early. The aim present study was evaluate whether oral fecal microbiomes an individual can be suitable for screening. Oral samples (n = 80) were gathered Taleghani hospital, affiliated with Shahid Beheshti University Medical Sciences, Tehran-Iran, from stage 0 I patients healthy controls (HCs), who...

Enterotoxigenic Bacteroides fragilis (ETBF) associated with the initiation and progression of colorectal cancer (CRC) has been alarmingly reported all over world. In this study, simultaneous investigation toxigenic non-toxigenic patterns I, II III biofilm formation ability isolated from patients was performed.Thirty-one diagnosed CRC thirty-one control subjects were recruited in study. Specimens cultured on BBE BBA culture media. Classical phenotypic identification tests PCR performed to...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause serious illness in older adults and people with chronic underlying medical conditions; however, children young are often asymptomatic or mild symptoms. We evaluated the presence of specific antibodies (Abs) response against Human NL63 (HCoV-NL63) S protein epitopes (NL63-RBM1, NL63-RBM2_1, NL63-RBM2_2, NL63-RBM3, NL63-SPIKE541–554, NL63-DISC-like) SARS-CoV-2 (COV2-SPIKE421–434 COV2-SPIKE742–759) plasma samples...

Chronic humoral immune response against multiple microbial antigens may play a crucial role in the etiopathogenesis of rheumatoid arthritis (RA). We aimed to assess prevalence and magnitude antibody various bacterial viral immunogen peptides sera RA patients compared with general population.Polyclonal IgG antibodies (Abs) specific for derived from Porphyromonas gingivalis (RgpA, Kpg), Aggregatibacter actinomycetemcomitans (LtxA1, LtxA2), Mycobacterium avium subsp. paratuberculosis (MAP4027),...

Comprehensive data on drug-resistant patterns of Acinetobacter baumannii isolates in developing countries is limited. We conducted a multihospital study to assess the rate and trend phenotypes Ac. using standardized definitions determine remaining therapeutic options against resistant phenotypes. The 401 nonduplicate were collected from six hospitals which are geographically distributed across Tehran, Iran over 3-year period. Following PCR blaOXA-51-like gene, susceptibility testing was...

Parkinson’s disease (PD) is a neurodegenerative disorder involving the accumulation of alpha-synuclein (α-syn)/Lewy bodies in brain and -enteric nervous system. The etiology not well understood, but bacterial viral infections may contribute to pathogenesis PD. It has been suggested that gastrointestinal (GI) complications observed PD patients arise from dysbiosis, leading curli/α-syn deposits enteric Enteric bacteria secrete curli, functional amyloid peptide involved adhesion surfaces, cell...

Systemic rheumatic diseases, including conditions such as rheumatoid arthritis, Sjögren’s syndrome, systemic sclerosis, and lupus erythematosus, represent a complex array of autoimmune disorders characterized by chronic inflammation diverse clinical manifestations. This study focuses on unraveling the genetic underpinnings these diseases examining polymorphisms in key genes related to their pathology. Utilizing comprehensive analysis, we have documented involvement variations pathogenesis...

Human endogenous retroviruses (HERVs) have been thought of as silent passengers within our genomes, but their reactivation has linked with several autoimmune diseases, including type 1 diabetes (T1DM). In order to evaluate the potential role HERVs, in addition recognized HERV-W, we focused on debated HERV-K family T1DM. Therefore, performed a serological evaluation IgG antibodies against Env epitope (HERV-K Env19−37) comparison an important β-cellular autoimmunity biomarker, ZnT8, from...

Recent studies have recognized the ATPase-encoding <i>comM</i> gene as a hot spot for integration of <i>Acinetobacter baumannii</i> resistance islands (RIs). Despite circulation high numbers multidrug-resistant <i>A. baumannii </i>(MDR-AB) isolates in Middle East countries, no information is available about interruption and subsequent transposition into belonging to global clones (GC) GC1, GC2, or GC3. In this study 401 from hospitals Tehran, Iran, were...

Background: Nocardia, Streptomyces and Rhodococcus are life threatening opportunistic pathogens under immunodeficiency conditions, particularly among patients infected with HIV. Rapid accurate detection of these infections can improve immune health quality, patient management appropriate treatment. The aim this study was to design a novel multiplex-PCR assay for rapid diagnosis three organisms directly from bronchoalveolar lavage (BAL) specimens HIV.Methods: genus specific primers were...

<title>Abstract</title> Background Human Endogenous retroviruses (HERVs) constitute about 8% of the human genome. Overexpression HERVs has been detected in various inflammatory disorders like neuroinflammation and cancer. Interestingly, it reported that stress conditions facilitate expression. Space travel will expose astronauts to microgravity environments (a condition), which may result activation might influence pathogenic outcomes during after space flight. This study aimed elucidate...

Abstract Human endogenous retroviruses (HERVs) involvement in neurological diseases has been extensively documented, although the etiology of HERV reactivation remains unclear. MicroRNAs represent one potential regulatory mechanisms reactivation. We identified fourteen microRNAs predicted to bind HERV-K transcript, and subsequently analyzed for their gene expression levels alongside those HERV-K. documented an increased four patients with Parkinson’s disease compared healthy controls, which...

Brucellosis, a major health problem in developing countries, is multisystem infection with broad spectrum of clinical manifestations. Hematological complications, ranging from an intravascular coagulopathy to mild homeostasis disorders (such as gammopathy), have been reported brucella infection. These signs and symptoms may lead misdiagnosis brucellosis other hematological diseases.A 65-year-old male whose occupation was shepherding referred our hospital known case multiple myeloma...

Background: Urinary tract infection (UTI) is one of the most common type bacterial infectious diseases which occurs in all age groups. The aim this study was to determine anti-microbial resistance pattern pathogens causing UTIs hospitalized patients at Imam Khomeini Hospital, Tehran, Iran. Material and Methods: Urine samples were collected from 11157 different wards Hospital between January 2015 December 2015.The cultured plates assessed for significant growth. Anti-microbial susceptibility...