Background: The emergence of the plasmid-mediated mcr colistin-resistance gene in bacteria poses a potential threat for treatment patients, especially when hospitalized. aims this study were to search presence mcr-1 and mcr-2 genes among colistin-resistant Escherichia coli ( E. ) Klebsiella pneumoniae K. isolates from clinical specimens determine fingerprint strains by enterobacterial repetitive intergenic consensus sequences PCR (ERIC-PCR) method. Methods: In study, 712 nonduplicate...

Carbapenem-resistant Acinetobacter baumannii has emerged as an important cause of infection in burn patients. This study aimed to characterize the antimicrobial susceptibility pattern, determine prevalence oxacillinase and metallo-beta-lactamase (MBL) genes, type A. isolates obtained from patients.During a 1-year period, total 40 nonduplicated were patients who hospitalized Taleghani Burn Hospital Ahvaz, southwest Iran. Testing for was carried out by disk diffusion E-test. To screen MBL...

Chlamydia trachomatis, Ureaplasma parvum, and Mycoplasma genitalium are common sexually transmitted microorganisms. Our study aimed to determine the prevalence of C. U. M. in infertile fertile couples effect these microorganisms on semen parameters.

Antibiotic resistant especially to Extended Spectrum Beta-Lactamases (ESBLs) producing bacteria has been known as a healthy problem in treatment of patients with infection. The aim this study is determine the antimicrobial resistance patterns ofEnterobacteriaceae isolated from clinical samples and also detect phenotypic genotypic ESBLs organisms. In study, 420 Enterobacteriaceae strains isolated 8000 specimens were identified by biochemical standard tests. Their primary susceptibility was...

Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen. We sought to determine the frequency of different types SCCmec in MRSA isolates by performing cross-sectional study. A total 72 S. were collected from Imam Khomeini and Golestan hospitals analysed for typing multiplex PCR. The pattern antibiotic resistance among was determined disc diffusion analysis. Of isolates, 29 (40.27%) recognized as MRSA. type III most common type, with 55.17% (16/29), followed II...

Objective: Nosocomial infections due to vancomycin-resistant enterococci (VRE) are known as a source of spreading these bacteria. The aim this prospective study was molecular detection vanA and vanB genes among VRE isolated from patients admitted intensive care units (ICUs) in Ahvaz southwest Iran. Materials methods: Overall, 243 non-duplicate rectal swab specimens were collected ICU-hospitalized teaching hospitals affiliated Jundishapur University Medical Sciences, inoculated on suitable...

Staphylococcus aureus, an important human pathogen is one of the main causative agents nosocomial infection. Virulence genes play a major role in pathogenicity this agent and its infections. Methicillin-Resistant aureus (MRSA) isolates are challenge among infectious that can cause severe infections mortality. Methicillin-resistant S. produces unique type Penicillin Binding Protein 2a (PBP2a) has low affinity for β-lactam antibiotics. Most MRSA bacterial strains also produce leukotoxin as...

Recently Acinetobacter baumannii isolates have emerged as a problematic infectious agent that causes meningitis in neurosurgical patients. Colistin has been used successfully for the treatment of A. but colistin resistant reported worldwide.Two were cultured during five-day period from cerebrospinal fluid (CSF) samples 20-year-old man with gunshot trauma abdomen, which had exited his back. Antimicrobial susceptibility tests performed. Multiplex PCR was performed detection bla OXA-23-like,...

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most pathogens associated with health care. Molecular typing methods are vital for outbreak investigations MRSA. The aim this study was characterization SCCmec, spa types and multidrug resistant methicillin-resistant isolates in Ahvaz, Iran.A total 50 MRSA were determined by using phenotypic method mecA gene. Antibiotic resistance profile SCCmec screened disc diffusion PCR, respectively. For isolates, two molecular including...

Acinetobacter baumannii is one of the most important organisms in nosocomial infections. Antibiotic resistance this bacterium causes many problems treating patients. This study aimed to investigate antibiotic patterns and resistance-related, genes clinical isolates baumannii. descriptive was conducted on 124 collected from samples two teaching hospitals Ahvaz. The pattern determined by disk diffusion. presence coding for using polymerase chain reaction method. Out isolates, highest rate...

Carbapenem resistant Acinetobacter baumannii is an important nosocomial pathogen associated with a variety of infections.The current study aimed to characterize the antimicrobial susceptibility, analyze prevalence oxacillinase and metallo-β-lactamase (MBL) genes molecular typing clinical isolates A. baumannii.A total 124 non-repetitive were collected from various specimens in two teaching hospitals Ahvaz, south-west Iran. Antimicrobial susceptibility test was carried out by disk diffusion...

The emergence of multidrug-resistant Escherichia coli has become a great challenge in treating nosocomial infections. polymyxin antibiotic colistin is used as 'last-line' therapy for such strains, but resistance to increasingly emerging all over the world. In this study, we investigated lipopolysaccharides (LPS) colistin-resistant isolates and examined mutations lpx genes strains not harbouring mcr genes. We 351 clinical E. with 38 showing reduced susceptibility colistin. These were...

Background: Although infection with Helicobacter pylori is a global health problem, its prevalence different among countries. Serologic tests for the diagnosis of H. are limited by low accuracy rates and lack validation. Recent studies indicate that stool antigen test has an acceptable level accuracy. Objectives: Our objective was to investigate in Khuzestan province, south-west Iran evaluate possible risk factors infection. Patients Methods: In this study, from September October 2009, 861...

Background: Legionnaires' disease is an important public health problem that can cause substantial mortality and morbidity. disease-risk estimation may be compromised by uncertainties in Legionella -detection methods. The aim of this study was the detection L. pneumophila respiratory specimens hospitalized patients with symptoms culture, PCR, loop-mediated isothermal amplification (LAMP) Methods: Sputum bronchoalveolar lavage samples were obtained from pneumonia admitted to teaching...

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Antibiotic resistance mechanisms in Enterobacteriaceae are causative agents of global health problems. Bacterial infections due to multidrug (MDR) may be mediated by the overexpression efflux pumps. In this study, we investigated prevalence oqxA and oqxB genes as two encoding pumps determination antibiotic rate clinical isolates Enterobacteriaceae. 100 collected from different specimens infectious patients, such wounds, urine, blood, discharge, abscesses except stool, were examined....

Background: Infections due to Mycoplasmatales members can cause infertility, preterm delivery and neonatal morbidity mortality. Therefore, rapid diagnosis is of great importance control these infections diminish their outcomes. Objectives: The current study aimed develop the multiplex PCR assay detect two genital mollicutes from a single amplification reaction relation with habitual abortion urogenital infection in infected females. Materials Methods: Urine samples symptomatic females (20-54...

Background: SPATE (serine protease autotransporters of enterobacteriaceae) genes are considered as a group the main virulence factors Shigella species This study aimed to investigate for first time distribution among spp. isolated from children with diarrhea infection in Ahvaz, Iran. Methodology: In this study, total 74 isolates were collected between August 2016 and June 2017 feces identified by biochemical molecular methods species. The frequency genes, including pic, pet, sat, sigA sepA ,...