A5038240731- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Infectious Diseases and Tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antifungal resistance and susceptibility
- Actinomycetales infections and treatment
- Escherichia coli research studies
- Quinazolinone synthesis and applications
- Antibiotic Use and Resistance
- Bacteriophages and microbial interactions
- Garlic and Onion Studies
- Antimicrobial agents and applications
- Urinary Tract Infections Management
- Vibrio bacteria research studies
- Fungal Infections and Studies
- Legionella and Acanthamoeba research
- Amoebic Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Nephrotoxicity and Medicinal Plants
Ahvaz Jundishapur University of Medical Sciences
2015-2024
Tropical Diseases Research Centre
2020
Pasteur Institute of Iran
2013
This study investigated the molecular epidemiology of carbapenem-resistant classic
The aim of this multicenter study was to determine the genetic diversity and antibiotic susceptibility clinically isolated Nocardia species. One hundred twenty-seven patients with nocardiosis were randomly selected from 5 provinces Iran. Molecular diagnosis species performed using multilocus sequence analysis gyrase B β subunit DNA topoisomerase (gyrB), 16S rRNA A SecA preproteintranslocase (secA1). Antimicrobial testing following Clinical Laboratory Standards Institute recommendations....
Many species of nontuberculous mycobacteria (NTM) have long been identified as important causes human disease, the incidence which is rising. Several reports suggested increasing trend both in vitro and vivo resistance to available treatment regimes. The aim this study was evaluate antibiotic susceptibility clinically relevant NTM isolates using standard microbroth dilution test. Antimicrobial testing performed following National Committee for Clinical Laboratory Standards methods isolates,...
Enteroaggregative Escherichia coli (EAEC) has been implicated as an emerging cause of traveler's diarrhea, persistent diarrhea among children, and immunocompromised patients. The present study aimed to investigate the prevalence antibiotic resistance, extendedspectrum β-lactamase (ESBL) production, virulence factors EAEC isolates obtained from Iranian children suffered diarrhea.In this cross-sectional study, March 2015 February 2016, 32 were collected fecal samples aged <12 years with in...
Tuberculosis (TB) remains as one of the most serious infectious diseases in world. Pulmonary tuberculosis can occur with other pulmonary caused by opportunistic organisms such Nocardia spp. particularly immunocompromised patients. Therefore, diagnosis co-infection at early stage disease could be lifesaving.The goal this study was to detect Mycobacterium and sputum specimens order assess concomitant nocardiosis patients suspected tuberculosis.From March 2011 April 2012, 189 were obtained from...
Pyogenic spinal infection continues to represent a worldwide problem. In approximately one-third of patients with pyogenic spondylodiscitis, the infectious agent is never identified. Of cases that lead organismal identification, bacteria are more commonly isolated from spine rather than fungi and parasites. This study applied universal prokaryotic 16S rRNA PCR as rapid diagnostic tool for detection bacterial agents in specimens suspected spondylodiscitis. Gram Ziehl-Neelsen staining were...
Nontuberculous mycobacteria (NTM) are among the emerging pathogens in immunocompromised individuals including hospitalized patients. So, it is important to consider hospitals water supplies as a source for infection. The aim of this study was determine prevalence NTM hospital aquatic systems Khuzestan, South west Iran. In total, 258 samples were collected and examined. After initial sample processing, sediment each inoculated into two Lowenstein-Jensen medium. positive cultures studied with...
Oral candidiasis (OC) is one of the most common opportunistic fungal infections among people living with HIV/AIDS (PLWHA). The prevalence OC and Candida profiles HIV-infected patients might be changing in era Highly Active Antiretroviral Therapy (HAART). This study aimed to identify spp., determine associated risk factors for PLWHA.Oral was explored oral swabs 276 who referred consultation at Behavioral Diseases Counseling Center (BDCC). Clinical symptoms, culture molecular assays were used...
Staphylococcus saprophyticus is the second most frequent community-acquired causative agent of acute urinary tract infection (UTI). Some strains S. can create biofilms, increasing their virulence. Once biofilms have been produced, antibiotic resistance exacerbated. Hence, aims present study were biofilm formation, structure and in causing UTIs women Ahvaz, Iran. Overall, 43 isolates recovered from UTIs. Antibiotic pattern production determined using phenotypic methods. Most resistant to...
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most pathogens associated with health care. Molecular typing methods are vital for outbreak investigations MRSA. The aim this study was characterization SCCmec, spa types and multidrug resistant methicillin-resistant isolates in Ahvaz, Iran.A total 50 MRSA were determined by using phenotypic method mecA gene. Antibiotic resistance profile SCCmec screened disc diffusion PCR, respectively. For isolates, two molecular including...
Six strains of a rapidly growing scotochromogenic mycobacterium were isolated from pulmonary specimens independent patients. Biochemical and cultural tests not suitable for their identification. The mycolic acid pattern analysed by HPLC was different that any other mycobacterium. Genotypic characterization, targeting seven housekeeping genes, revealed the presence microheterogeneity in all them. Different species more closely related to test various regions: type strain Mycobacterium...
Four strains isolated in Iran from pulmonary specimens of unrelated patients are proposed as representative a novel Mycobacterium species. Similarity, at the phenotypic level, with kansasii is remarkable photochromogenic yellow pigmentation colonies being salient feature. They differ, however, genotypically this species and present unique sequences 16S rRNA, hsp65 rpoB genes. The average nucleotide identity genome-to-genome distance fully support status an independent name for persicum sp....
The global rise in drug-resistant Mycobacterium tuberculosis (M.tb), and especially the significant prevalence of isoniazid (INH)-resistance constitute a challenge to health. Therefore, present study aimed investigate mutations prevalent gene loci-involved INH-resistance phenotype-among M.tb clinical isolates from southwestern Iran.Drug susceptibility testing (DST) was performed using conventional proportional method on confirmed 6620 isolates, total, 15 INH-resistant 18 INH-susceptible were...
There are limited studies on the antibiotic resistance patterns of slowly growing mycobacteria (SGM) species and their related gene mutations in Iran. This study aimed to elucidate susceptibility profiles some genes that associated with among SGM isolates from The strains were isolated sputum samples suspected tuberculosis (TB) patients. identified by standard phenotypic tests assigned level amplification sequencing dnaK gene. minimum inhibitory concentration (MIC) eight antibiotics was...
Nontuberculous mycobacterial (NTM) infections are growing worldwide especially in immunocompromised individuals. Since treatment of NTM is species-specific, the precise identification to species level critical for an optimal treatment. This study was aimed identify different by sequencing rpoB gene and evaluating effectiveness argH cya markers. In total 64 clinical isolates suspected were collected. The done standard conventional methods PCR-based sequence analysis. PCR genes performed...
Abstract Tuberculosis (TB) is considered as one of the most important infectious diseases in world, and recent rise spread multidrug‐resistant (MDR) Mycobacterium tuberculosis (MTB) strains, have made matter worsened. Due to importance TB prevalence Iran, this study was designed investigate genetic diversity among MDR strains MTB by MIRU‐VNTR typing scheme. A total 88 drug resistant M. isolates belong pulmonary cases were collected from several reference centers Iran. Drug susceptibility...
Infections caused by nontuberculous mycobacteria (NTM) is increasing wordwide. Due to the difference in treatment of NTM infections and tuberculosis, rapid species identification mycobacterial clinical isolates necessary for effective management diseases their control strategy. In this study, a cost-effective technique, real-time PCR coupled with high-resolution melting (HRM) analysis, was developed differentiation Mycobacterial using novel rpoBC sequence. A total 107 (Nine reference 98...
The Non-tuberculous mycobacterial (NTM) isolates should be distinguished from tuberculosis and identified at the species level for choosing an appropriate treatment plan. In this study, two molecular methods were used to differentiate NTM species, including a new designed High Resolution Melting (HRM) Multilocus Sequence Analysis (MLSA). Seventy-five evaluated by sequencing four genes ( MLSA) HRM assay specifically targeting atpE was rapidly accurately identify mycobacterium species. Out of...
The characterization of five Iranian isolates, four from hospital haemodialysis water and one the sputum a patient, led to detection novel mycobacterium species. strains were characterized by mucoid colonies developing in 3-5 days at temperatures ranging 25 37 °C. biochemical test pattern was unremarkable while HPLC profile mycolic acids resembled that Mycobacterium fortuitum. sequences three major housekeeping genes (16S rRNA, hsp65 rpoB) unique differed those any other mycobacterium....
Tuberculosis (TB) is one of the leading causes mortality among infectious diseases and accounts for a serious health hazard wordwide. Apart from TB, members non-tuberculous mycobacteria (NTM), which includes around 170 species, may also cause different in humans. Therefore this study aimed to investigate distribution NTM strains isolated extrapulmonary (EP) samples by Real-Time PCR PCR-sequencing methods Southwest Iran. Three hundred twenty-five suspected EP were collected patients referred...