Goats are globally invaluable ruminants that balance food security and environmental impacts, their commensal microbiome residing in the gastrointestinal tract (GIT) is associated with animal health productivity. However, reference genomes functional repertoires of GIT microbes goat kids have not been fully elucidated. Herein, we performed a comprehensive landscape survey using metagenomic sequencing binning, spanning dense sampling regime covering three compartments spatially five...

In ruminants, a symbiotic rumen microbiota is responsible for supporting the digestion of dietary fiber and contributes to health traits closely associated with meat milk quality. A holistic view physicochemical profiles mixed (MRM) not well-illustrated. The experiment was performed 3 × 4 factorial arrangement specific surface area (SSA: 3.37, 3.73, 4.44 m2/g) NDF extracted from rice straw tension (ST: 54, 46, 43, 36 dyn/cm) fermented medium in fermentation time series 6, 12, 24, 48 h three...

Previous studies have discussed the association between serum metabolism and lactation performance among Sanhe Holstein cows of different parities found that metabolic profiles these two breeds vary differently with parity. Since rumen is central organ for nutrient absorption production transformation in dairy cows, it remains unknown whether differences observed under same dietary conditions are related to structure microbiome. This study measured apparent digestibility fermentation...

The performance of dairy cows is closely tied to the metabolic state, and this varies depending on number times have given birth. However, there still a lack research relationship between state Holstein lactation across multiple parities. In study, biochemical analyses metabolomics studies were performed serum milk from parities 1–4 (H1, N = 10; H2, 7; H3, 9; H4, 9) in mid-lactation (DIM 141 ± 4 days) investigate link changes. results quality analysis showed that lactose levels highest H1 (p...

The production performance of dairy cattle is closely related to their metabolic state. This study aims provide a comprehensive understanding the and features Sanhe across different parities, with specific focus on evaluating variations in milk traits metabolites both serum. from parities 1 4 (S1, n = 10; S2, 9; S3, S4, 10) at mid-lactation were maintained under same feeding management conditions. traits, hydrolyzed amino acid levels, serum biochemical parameters, free levels determined....

Skeletal muscle cells (SMCs) of goats were stress induced with 1 mM H(2)O(2) in the absence or presence 0.5, 5, and 50 μg/mL tea catechins (TCs) incubation. Cells harvested at 48 h postincubation TCs to investigate effects on cell proliferation, membrane integrity, antioxidant enzyme activities, genes protein expression levels. Results showed that induction inhibited proliferation without TC incubation; moreover, inhibition effect was enhanced (P < 0.001). H(2)O(2)-induced increased lactate...

Feeding a growing global population and lowering environmental pollution are the two biggest challenges facing ruminant livestock. Considering significance of nitrogen metabolism in these challenges, dietary intervention regarding metabolizable protein profiles with different rumen-undegradable (RUP) ratios (high RUP [HRUP] versus low [LRUP]) was conducted young ruminants weaned goats as model. Fecal samples were collected longitudinally for nine consecutive weeks to dissect timing duration...

A high-grain (HG) diet can result in ruminal subacute acidosis, which is detrimental to gut health and lead decreased productivity. This study investigated the ileal epithelial microbiota its relationship with host function goats fed a HG (concentrate/hay, 90:10) control 55:45), aiming elucidate mechanisms involved adaptation acidosis. The challenge increased volatile fatty acid concentration (p = 0.030) altered by increasing (FDR < 0.05) relative abundances of active carbohydrate protein...

The gut microbiome plays important roles in the regulation of gastrointestinal tract functional development and host mucosal immune maturation. This study was conducted to test hypothesis that age feeding system (supplemental [Sup] vs. grazing [G]) could alter colonic bacterial diversity Thirty Liuyang black goat kids ( = 4) were slaughtered on d 0, 7 (nonrumination), 28, 42 (transition), 70 (rumination). microbiota profiled by Miseq sequencing 16S rRNA gene. Host maturation examined using...

In high-altitude area, hypoxia is a serious stress for humans and other animals, disrupting oxygen homeostasis thus affecting tissue metabolism. Up to now, there are few reports on the metabolic changes of dairy cows at different altitudes. this experiment, metabonomics technology blood biochemical indexes were used study in The results showed that metabolites mainly enriched amino acid metabolism sphingolipid metabolism, negative correlation with increased altitude. will enrich...

Abstract This study measured gaseous emissions, growth performance and pork quality in a deep‐litter system concrete‐floor system. Three hundred twenty weaned piglets with an average body weight ( BW ) of 6.0 ± 0.3 kg were assigned randomly into three treatments. Treatments 1 2 included four pens 20 pigs for each pen respectively the system, ratio sawdust to chaff was 5:5 3:7 treatments respectively, probiotics inoculated fermentation bedding both composed Saccharomycetes , Bacillus subtilis...

Changes in the environment such as high-altitude hypoxia (HAH) can lead to adaptive changes blood system of mammals. However, there is limited information about adaptation Holstein dairy cows introduced areas. This study used 12 multiparous (600 ± 55 kg, average three years old) exposed HAH conditions Nyingchi Tibet (altitude 3000 m) and HAH-free Shenyang 50 m). The miRNA microarray analysis iTRAQ proteomics approach (accepted more suitable for accurate comprehensive prediction targets) were...

The mammary gland of dairy cows is a formidable lipid-synthesizing machine for lactation. This unique function depends on the activities plasma membrane (PM) proteins in cells. Little information known about expression profiles PM and their functions during lactating process. study investigated proteome map fractions using 1D-Gel-LC-MS/MS identified 872 nonredundant with 141 unknown proteins, wherein 215 were PM-associated proteins. Most binding, transport, catalytic such as annexin heat...

At present, feeding a high-corn diet to goats is used provide enough protein and energy supply meet their higher dietary requirements. In fact, because corn grain commonly scarce in the traditional rice cropping region of southern Asia, paddy thereby as an alternative feed applied goat diets. However, effects high proportion on microbiota metabolites intestine are unclear. Here, we investigate bacterial community, potential function, metabolic reaction cecum goats. Sixteen Liuyang black were...

Thirty-six Xiangdong black goats were used to investigate age-related mRNA and protein expression levels of some genes related skeletal muscle structural proteins, MRFs MEF2 family, fiber type composition during growth under grazing (G) barn-fed (BF) feeding systems. Goats slaughtered at six time points selected reflect developmental changes nonrumination (days 0, 7, 14), transition (day 42), rumination phases 56 70). It was observed that the number IIx in longissimus dorsi increased quickly...

Ramie (Boehmeria nivea) is noted for the production of a large biomass that has high protein content and rich in antioxidants. It may thus serve as high-quality forage material to replace alfalfa improve meat quality farmed animals. In this study, we evaluated carcass characteristics goats when 0, 35%, 75%, 100% dietary was replaced with ramie. Crude (linear, P < 0.0001) key muscle color values at 24 h after slaughter decreased increasing ramie levels. The most individual amino acids,...

Quorum sensing (QS) is a type of microbe-microbe communication system that widespread among the microbial world, particularly microorganisms are symbiotic with plants and animals. Thereby, cell-cell signalling likely to occur in an anaerobic rumen environment, which complex ecosystem. In this study, using six ruminally fistulated Liuyang black goats as experimental animals, we aimed detect activity quorum autoinducers (AI) both vivo vitro clone luxS gene encoded autoinducer-2 (AI-2) synthase...

1. Conjugated linoleic acid supplementation during late gestation and lactation of sows affects myofiber type in their litters Renli Qi, Ying Chen, Han Peng, Shan Jiang, Rong Xiao, Jinxiu Huang Livestock Science CrossRef

The dual stress of reduced feed intake and increased milk yield in dairy cows early postpartum results a negative energy balance. Rumen-protected glucose (RPG) has been reported to replenish energy, increase yield, improve gut health. However, often develop an insulin resistance, implying that RPG may not be well utilized production the liver's fat oxidization burden. This study aimed investigate effects on hepatic oxidative/antioxidative status protein profile. Starting 7 d before expected...