A5056994259- Ruminant Nutrition and Digestive Physiology
- Gut microbiota and health
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Adipose Tissue and Metabolism
- Effects of Environmental Stressors on Livestock
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Fatty Acid Research and Health
- Amino Acid Enzymes and Metabolism
- Infant Nutrition and Health
- Cancer-related molecular mechanisms research
- Anaerobic Digestion and Biogas Production
- Muscle metabolism and nutrition
- Biochemical Analysis and Sensing Techniques
- Milk Quality and Mastitis in Dairy Cows
- Agroforestry and silvopastoral systems
- Pasture and Agricultural Systems
- Metabolism, Diabetes, and Cancer
- Clinical Nutrition and Gastroenterology
- Meat and Animal Product Quality
- Bacteriophages and microbial interactions
- Folate and B Vitamins Research
- Plant and fungal interactions
- Microbial infections and disease research
Chinese Academy of Sciences
2017-2025
Institute of Subtropical Agriculture
2016-2025
Fuzhou University
2024
University of Chinese Academy of Sciences
2013-2024
Hunan Vocational College of Safety Technology
2016-2017
Icahn School of Medicine at Mount Sinai
2014
Northwest A&F University
2012
Abstract Ruminants are important for global food security but emit the greenhouse gas methane. Rumen microorganisms break down complex carbohydrates to produce volatile fatty acids and molecular hydrogen. This hydrogen is mainly converted into methane by archaea, can also be used hydrogenotrophic acetogenic respiratory bacteria useful metabolites. A better mechanistic understanding needed on how dietary influence metabolism methanogenesis. We profiled composition, metabolic pathways,...
ABSTRACT Understanding of the colonization process epithelial bacteria attached to rumen tissue during development is very limited. Ruminal bacterial great significance for relationship between microbiota and host can influence early health host. MiSeq sequencing 16S rRNA genes quantitative real-time PCR (qPCR) were applied characterize ruminal diversity in this study. Seventeen goat kids selected reflect no-rumination (0 7 days), transition (28 42 rumination (70 days) phases animal...
Goats are globally invaluable ruminants that balance food security and environmental impacts, their commensal microbiome residing in the gastrointestinal tract (GIT) is associated with animal health productivity. However, reference genomes functional repertoires of GIT microbes goat kids have not been fully elucidated. Herein, we performed a comprehensive landscape survey using metagenomic sequencing binning, spanning dense sampling regime covering three compartments spatially five...
The aim of the present study was to describe age-related changes in anatomic, functional and microbial variables during rumen development process, as affected by feeding system (supplemental v . grazing), goats. Goats were slaughtered at seven time points that selected reflect non-rumination (0, 7 14 d), transition (28 42 d) rumination (56 70 phases development. Total volatile fatty acid (TVFA) concentration ( P = 0·002), liquid-associated bacterial archaeal copy numbers < 0·01) greater...
The objective of this present study was to explore the initial establishment metabolically active bacteria and subsequent evolution in four fractions: rumen solid-phase (RS), liquid-phase (RL), protozoa-associated (RP), epithelium-associated (RE) through early weaning supplementing rhubarb root powder 7 different age groups (1, 10, 20, 38, 41, 50, 60 d) during development. Results 16S rRNA sequencing based on RNA isolated from fractions revealed that potentially bacterial microbiota were...
Abstract Ruminants are essential for global food security, but these major sources of the greenhouse gas methane. Methane yield is controlled by cycling molecular hydrogen (H2), which produced during carbohydrate fermentation and consumed methanogenic, acetogenic, respiratory microorganisms. However, we lack a holistic understanding mediators pathways H2 metabolism how this varies between ruminants with different methane-emitting phenotypes. Here, used metagenomic, metatranscriptomic,...
The microbes residing in ruminant gastrointestinal tracts play a crucial role converting plant biomass to volatile fatty acids, which serve as the primary energy source for ruminants. This tract comprises foregut (rumen) and hindgut (cecum colon), differ structures functions, particularly with respect feed digestion fermentation. While rumen microbiome has been extensively studied, cecal remains much less investigated understood, especially concerning assembling microbial communities...
Abstract Background Dietary protein level and amino acid (AA) balance are crucial determinants of animal health productivity. Supplementing rumen-protected AAs in low-protein diets was considered as an efficient strategy to improve the growth performance ruminants. The colon serves a conduit for nutrient metabolism during methionine (RPMet) lysine (RPLys) supplementation, however, it has been challenging clarify which specific microbiota their metabolites play pivotal role this process....
Improving nitrogen utilization efficiency in ruminant livestock is vital for feeding a growing global population and lowering environmental pollution. The rumen intestine harbor distinct epithelial structures biogeographically stratified microbiota, their co-oscillation assemblage patterns fulfill the pivotal role of metabolizing dietary into bioavailable nutrients ruminants. There cursory evidence to suggest that an increased understanding spatial gastrointestinal microbiota‒host...
Abstract This study was performed to investigate the initial colonization of metabolically active methanogens and subsequent changes in four fractions: rumen solid-phase (RS), liquid-phase (RL), protozoa-associated (RP), epithelium-associated (RE) from 1 60 d after birth, manipulate methanogen community by early weaning on 40 supplementing rhubarb black goats. The RNA-based real-time quantitative PCR 16S rRNA amplicon sequencing were employed indicate methanogens. Results showed that...
The establishment of gut microbiota is increasingly recognized as a crucial action in neonatal development, host health and productivity. We hypothesized that the ileal microbiome shifted goats matured, this colonization process would be associated with fermentation capacity. To end, 18 Liuyang black grazing were randomly slaughtered at d 0, 7, 28, 42, 70. Ileal was profiled by Miseq sequencing 16S rRNA gene bacteria, capacity [volatile fatty acid, activities amylase, carboxymethylcellulase...
BACKGROUND Post-ruminal digestion of fiber has received much less attention than its ruminal digestion. Using in vitro incubation techniques, the present study explored whether variations occurred different segments post-ruminal tract and structure could influence digestibility. A split plot design was conducted with gut (jejunum, ileum, cecum colon) as main substrates (neutral detergent (NDF) cellulose (CEL)) subplot. RESULTS With same substrate, final asymptotic gas volume (VF), production...
This study aimed to characterize the rumen microbiota structure of cattle grazing in tropical rangelands throughout seasons and their responses ecology productivity a N-based supplement during dry season. Twenty pregnant heifers season northern Australia were allocated either N-supplemented or un-supplemented diets monitored through seasons. Rumen fluid, blood, feces analyzed before supplementation (mid-dry season), after two months (late-dry post (wet season). Supplementation increased...
Abstract We tested the hypotheses that supplementation of a diet with elemental Mg increases ruminal dissolved H 2 (dH ) in rumen fluid, which turn alters fermentation and microbial community goats. In randomised block design, twenty growing goats were allocated to two treatments fed same basal 1·45 % Mg(OH) or 0·6 Mg. After 28 d adaptation, we collected total faeces measure tract digestibility, contents analyse end products groups, measured methane (CH 4 emission using respiration chambers....
The abuse and misuse of antibiotics in livestock production pose a potential health risk globally. Rhubarb can serve as alternative to antibiotics, several studies have looked into its anticancer, antitumor, anti-inflammatory properties. aim this study was test the effects rhubarb supplementation diet young ruminants on innate immune function epithelial microbiota small intestine. Goat kids were fed with control supplemented or without (1.25% DM) slaughtered at days 50 60 age. Results showed...
Feeding a growing global population and lowering environmental pollution are the two biggest challenges facing ruminant livestock. Considering significance of nitrogen metabolism in these challenges, dietary intervention regarding metabolizable protein profiles with different rumen-undegradable (RUP) ratios (high RUP [HRUP] versus low [LRUP]) was conducted young ruminants weaned goats as model. Fecal samples were collected longitudinally for nine consecutive weeks to dissect timing duration...
Gastrointestinal microbiota may play an important role in regulating host mucosal innate immune function. This study was conducted to test the hypothesis that age (non-rumination, transition and rumination) feeding type [Supplemental (S) vs. Grazing (G)] could alter ruminal microbial diversity maturation of system goat kids. MiSeq sequencing applied investigate composition diversity, RT-PCR used expression immune-related genes mucosa. Results showed higher (P < 0.05) relative abundances...
A high-grain (HG) diet can result in ruminal subacute acidosis, which is detrimental to gut health and lead decreased productivity. This study investigated the ileal epithelial microbiota its relationship with host function goats fed a HG (concentrate/hay, 90:10) control 55:45), aiming elucidate mechanisms involved adaptation acidosis. The challenge increased volatile fatty acid concentration (p = 0.030) altered by increasing (FDR < 0.05) relative abundances of active carbohydrate protein...
The objectives of this study were 1) to investigate the age-related changes in functional achievement (establishment fermentation capacity and enzyme activities) bacterial succession (selected species) cecum colon 2) assess effect feeding system (supplemental vs. grazing, Sup G) on hindgut development small ruminants. A total 44 Liuyang black goat kids randomly slaughtered at 0, 7, 14 d age (nonrumination period), 28 42 (transition 56 70 (rumination period). Intestinal contents sampled...