In this study, we have determined the prognostic value of presence micrometastases and metastases greater than 2 mm in regional lymph nodes for bitches with mammary carcinomas. The study involved 51 dogs diagnosed a single malignant epithelial tumour 4th or 5th gland. All animals underwent mastectomy; glands were removed together inguinal node. examined immunohistochemically using anti‐cytokeratin antibody, clone AE1/AE3. followed up every 6 months years after surgery to determine...

The aim of the present study was to determine proportions individual coagulase-negative Staphylococcus species in clinical and subclinical mastitis. material consisted 100 CNS isolates obtained from 223 milk samples collected cows with Coagulase-negative staphylococci constituted 44.8% all isolated microorganisms. were mammary gland secretions 86 farms Lublin region (Poland). Clinical mastitis found 20 whereas 66 (23.3% 76.7%, respectively). symptoms mild. forms concerned mainly first or...

The aim of the study was to evaluate concentrations Interleukin-6 (IL-6) in milk and serum healthy cows (HE) with mastitis caused by Streptococcus spp. blood samples were obtained from Holstein-Friesian (Lublin region, Poland). A total 43 28 15 selected for study. IL-6 levels HE ranged 6.09–80.24 pg/mL (median 26.6 pg/mL) significantly lower than both clinical subclinical (487.09 vs. CM, p < 0.001; 165.31 SCM, 0.001). concentration not different entire group cows, regardless whether...

Abstract In this study, we have determined serum neopterin levels in female dogs with primary malignant mammary tumours. The study involved 50 which had a tumours removed surgically (32 animals carcinoma, 12 sarcoma and 6 carcinosarcoma) 10 clinically healthy dogs. Serum were using commercial ELISA kit. mean lower the tumour groups than but differences statistically significant only carcinoma groups. decrease of may suggest their decreased cellular immunity. Moreover, it might indicate that...

The objective of this bovine peripheral blood study was a comparative assessment the phagocytic activity neutrophils and monocytes intracellular killing capacity from cows given no probiotic which were administered consisting

The aim of the present study was to determine concentration serum amyloid A (SAA) and activity ceruloplasmin (Cp) in milk from cows with subclinical mastitis caused by different pathogens. Eighty-four samples fourteen healthy were examined. SAA determined using commercial ELISA kit (Tridelta Development Ltd., Greystones, Wicklow, Ireland). Cp assessed spectrophotometrically, Rice method. results reveal that (with exception CNS) cow can be regarded as markers mastitis, irrespective...

Summary The aim of the present study was to describe some oxidative stress parameters in bitches suffering from spontaneusly occurring mammary gland tumours. experiment involved 28 which had tumours removed surgically (15 with malignant tumour and 13 benign tumour) as well 10 clinically healthy bitches. activities glutathione peroxidase (GSH‐Px) superoxide dismutase (SOD) were determined haemolysates erythrocytes derived animals. concentrations thiobarbituric acid reactive substances...

Contents Numerous studies reported an increase of oxidative stress increases in both women and female laboratory animals after ovariectomy. However, there is little information about the evaluation antioxidative/oxidative status ovariectomized dogs. The purpose this study was to examine changes markers ovariohysterectomy ( OHE ) included eighteen healthy mongrel Blood samples were collected immediately before surgery 14 30 days surgery. Following parameters intensity determined: erythrocyte...

Pyometra is a serious bacterial infection of the uterus affecting female dogs and manifests as an accumulation pus in uterine lumen. The aim study was to assess changes serum interleukin (IL)-6 IL-10 concentrations bitches with pyometra undergoing ovariohysterectomy. Blood samples were collected from healthy (controls) before surgery, 3 10 days after Before had significantly higher IL-6 than controls. After concentration decreased significantly. In dogs, showed significant increase surgery...

The aim of the study was to investigate serum and milk concentrations tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYNA), indoleamine 2,3-dioxygenase (IDO) activity in cows suffering from subclinical mastitis caused by coagulase-negative staphylococci (MSCNS). TRP kynurenines were determined high-performance liquid chromatography (HPLC), IDO calculated as KYN/TRP ratio. blood samples collected 40 midlactation Holstein-Fresian two herds Lublin region Poland. In 20 with mastitis,...

Ketosis is a common disease occurring during the first stage of lactation in highly productive dairy cows. The aim present study was comparative assessment selected pro-inflammatory cytokines (including tumor necrosis factor-α [TNF-α] and interleukin 6 [IL-6]), anti-inflammatory IL-10), acute-phase proteins (APPs) haptoglobin [Hp] serum amyloid A [SAA]), sera cows with subclinical ketosis (SCK), those clinical (CK), healthy cows.Thirty Holstein-Friesian breed were investigated. divided into...

The aim of the study was to analyse total protein (TP), casein (CAS), lactose (LAC), and fat content milk from cows with subclinical (SCM) clinical mastitis (CM) caused by Streptococcus spp.A 60 samples diseased 30 healthy were included in study. Milk taken Holstein-Friesian four dairy farms Lublin Province. bacteriological examination performed somatic cells count 1 mL determined using a SomaCount FC automatic cell counter. Determination TP, CAS, LAC, FAT FA levels carried out DairySpec FT...

Abstract Introduction The aim of the study was to compare selected leukocyte subpopulations and serum amyloid A (SAA) concentration in peripheral blood cows at different stages lactation. receiving a probiotic as dietary supplement compared with not it. Material Methods research conducted on 20 pregnant dairy randomly divided into two groups 10 each. experimental group consisted given feed supplement. control that were fed without supplementation. Blood drawn six times for testing: 7 days...

The effect of vitamins E, C and β-carotene supplementation in sows on the parameters antioxidative/oxidative status during postpartum period was investigated. Twenty four primiparous sows, divided into two groups (experimental control), were included study. After half-way point pregnancy until farrowing, each experimental sow received feed supplemented twice a week with 200 mg vitamin E 1000 C, additionally, 70 administered via intramuscular injection, day 14 7 before farrowing. control...