Insulin-like growth factor II (IGF-II) is a major fetal factor. The IGF-II gene generates multiple mRNAs with different 5′ untranslated regions (5′ UTRs) that are translated in differential manner during development. We have identified human family of three mRNA-binding proteins (IMPs) exhibit attachments to the UTR from translationally regulated leader 3 mRNA but unable bind constitutively 4 mRNA. IMPs contain unique combination two RNA recognition motifs and four hnRNP K homology domains...

Insulin-like growth factor II mRNA-binding protein 1 (IMP1) belongs to a family of RNA-binding proteins implicated in mRNA localization, turnover, and translational control.Mouse IMP1 is expressed during early development, an increase expression occurs around embryonic day 12.5 (E12.5).To characterize the physiological role IMP1, we generated IMP1-deficient mice carrying gene trap insertion Imp1 gene.Imp1 ؊/؊ were on average 40% smaller than wild-type heterozygous sex-matched...

Clusters of regularly interspaced short palindromic repeats (CRISPRs) Sulfolobus fall into three main families based on their repeats, leader regions, associated cas genes and putative recognition sequences viruses plasmids. Spacer sequence matches to different plasmids the Sulfolobales revealed some bias particularly for family III CRISPRs. Transcription occurs both strands five repeat-clusters acidocaldarius a repeat-cluster conjugative plasmid pKEF9. Leader strand transcripts cover whole...

H19 RNA is a major oncofetal 2.5-kilobase untranslated of unknown function. The maternally expressedH19 gene located 90 kilobase pairs downstream from the paternally expressed insulin-like growth factor II(IGF-II) on human chromosome 11 and mouse 7; due to their reciprocal imprinting identical spatiotemporal expression, it assumed that two genes are functionally coupled. Here we show contains four attachment sites for IGF-II mRNA-binding protein (IMP) with apparentKd values in 0.4–1.3 nm...

Insulin-like growth factor-II mRNA-binding proteins 1, 2 and 3 (IMP1, IMP2 IMP3) belong to a family of RNA-binding implicated in mRNA localization, turnover translational control. We examined their expression pattern during development murine human testis ovaries. In the mouse, IMPs were expressed male female gonadal cells at embryonic day 12.5 (E12.5). From E16.5, IMP1 IMP3 became restricted developing germ cells, whereas persisted interstitial cells. mature mouse ovaries, IMP1, detected...

Journal Article Antagonistic effects of apomorphine and haloperidol on rat striatal synaptosomal tyrosine hydroxylase Get access Jan Christiansen, Christiansen Research Laboratories, A/S Ferrosan, Sydmarken 1–5, DK-2860 Soeborg, Denmark Search for other works by this author on: Oxford Academic Google Scholar Richard F Squires Pharmacy Pharmacology, Volume 26, Issue 5, May 1974, Pages 367–369, https://doi.org/10.1111/j.2042-7158.1974.tb09293.x Published: 12 April 2011 history Received: 27...

The IMP3 RNA-binding protein is associated with metastasis and poor outcome in human cancer. Using solid cancer transcriptome data, we found that correlates HMGA2 mRNA expression. Cytoplasmic granules contain HMGA2, dose-dependently increases mRNA. regulated by let-7, let-7 antagomiRs make refractory to IMP3. Removal of target sites eliminates IMP3-dependent stabilization, IMP3-containing bodies are depleted Ago1-4 miRNAs. relationship between Hmga2 IMPs also exists the developing limb bud,...

Lack of IGF2 in mice results diminished embryonic growth due to cell proliferation. Here we show that mouse fibroblasts lacking the RNA-binding protein IMP1 (IGF2 mRNA-binding 1) have defective splicing and translation mRNAs, markedly reduced polypeptide production, The proliferation IMP1-null can be restored wild-type levels by vitro or re-expression IMP1, which corrects defects RNA translation. ability correct these is dependent on phosphorylation at Ser181, catalyzed cotranslationally...

The IGF-II mRNA-binding proteins (IMPs), which are composed of two RNA recognition motifs, (RRM) and four hnRNP K homology (KH) domains, have been implicated in subcytoplasmic localization mRNAs during embryogenesis. IMP family originated via gene duplications before the divergence vertebrates, homologues consisting only KH motifs identified Drosophila Caenorhabditis elegans. Here we characterise trafficking GFP-IMP1 fusion determine structural determinants for proper cytoplasmic...

Sucrose gradient and Northern analyses were used to study the translational status of endogenous multiple mRNAs encoding prepropeptide for human insulin-like growth factor II. The results showed that a minor 4.8-kilobase mRNA was exclusively engaged in synthesis on membrane-bound polysomes, whereas major 6.0-kilobase present cytoplasmic particle which stable EDTA. We conclude discrimination between is dictated by their different 5'-untranslated regions.

Background: A major problem in outpatient foot surgery is severe postoperative pain that not sufficiently treated by peroral analgesics. Methods: Sixty‐three patients underwent under spinal anesthesia. Immediately on conclusion of the operation, sciatic and saphenous nerve blocks were performed. The was blocked popliteal fossa with 30 ml ropivacaine 5 mg −1 a perineural catheter passed through cannula. randomized to receive either 2 (n=30) or saline disposable elastomeric pump fixed infusion...

Insulin-like growth factor II (IGF-II) mRNAs are cleaved by an endonucleolytlc event in a conserved part of their 3' untranslated region that is predicted to exhibit complex higher-order RNA structure. In the present study, we have examined putative secondary structures vitro transcripts from human and rat enzymatic chemical probing. The results show cleavage site Is situated between two highly structured domains. upstream domain consists large hairpins, whereas downstream guanoslne-rlch....

The overlapping transcription units constituting the rat insulin-like growth factor II (IGF-II) locus generate multiple mRNAs by using different promoters. Three promoters have been identified, giving rise to 4.6-, 3.8-, and 3.6-kilobase mRNAs. latter, originating from promoter P3, is most abundant IGF-II mRNA in liver cell-line BRL-3A. Moreover, a non-polyadenylated 1.2-kilobase (kb) transcript 1.8-kb tail fragment are prominent transcripts at steady-state. In this study, we show that...

Mouse coding region determinant–binding (mCRD-BP) and human IGF-II mRNA–binding 1 (hIMP-1) proteins are orthologous mRNA-binding that recognize c-myc mRNA, respectively, regulate their expression posttranscriptionally. Here, we confirm CRD-BP/IMP-1 binds to mRNA it is predominantly expressed in fetal tissues. Moreover, hCRD-BP/IMP-1 was detected cell lines of neoplastic origin selected primary tumors. In a series 33 malignant 10 benign mesenchymal tumors, 73% 40%, were found express...

Post-transcriptional RNA regulons ensure coordinated expression of monocistronic mRNAs encoding functionally related proteins. In this study, we employ a combination RIP-seq and short- long-wave individual-nucleotide resolution crosslinking immunoprecipitation (iCLIP) technologies in Drosophila cells to identify transcripts associated with cytoplasmic ribonucleoproteins (RNPs) containing the RNA-binding protein Imp. We find extensive binding Imp 3′ UTRs that are involved F-actin formation. A...