A5085155818- Glycosylation and Glycoproteins Research
- Protein Tyrosine Phosphatases
- Peptidase Inhibition and Analysis
- HER2/EGFR in Cancer Research
- Lung Cancer Treatments and Mutations
- Cancer Research and Treatments
- Chronic Lymphocytic Leukemia Research
- Cancer-related Molecular Pathways
- Lung Cancer Research Studies
- Cancer, Hypoxia, and Metabolism
- Mechanisms of cancer metastasis
- Nanoplatforms for cancer theranostics
- Glioma Diagnosis and Treatment
- Advanced Drug Delivery Systems
- Nanoparticle-Based Drug Delivery
- Microtubule and mitosis dynamics
- Neuroblastoma Research and Treatments
Yale University
2018
Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins. However, targeting cancer therapy has not been feasible due to generalized effects on all glycoproteins. Here, we perform sensitivity screening 94 lung cell lines using NGI-1, small-molecule inhibitor oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate selective loss tumor viability. This screen revealed NGI-1 in...
Abstract Purpose: Parallel signaling reduces the effects of receptor tyrosine kinase (RTK)–targeted therapies in glioma. We hypothesized that inhibition protein N-linked glycosylation, an endoplasmic reticulum co- and posttranslational modification crucial for RTK maturation activation, could provide a new therapeutic approach glioma radiosensitization. Experimental Design: investigated small-molecule inhibitor oligosaccharyltransferase (NGI-1) on EGFR family receptors, MET, PDGFR, FGFR1....
<p>Deep sequencing of lung cancer cell lines to determine the proportion EGFR mutations.</p>
<p>Characterization of the Osimertinib resistant H1975 cells.</p>
<p>Legends for supplementary figures 1 and 2</p>
<p>Results of the Lung Cancer Cell Line Screen for OST Inhibitor Sensitivity</p>
<p>Apoptosis susceptibility of KRAS mutant A549 cell line following treatment with NGI-1, Erlotinib or both.</p>
<div>Abstract<p>Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins. However, targeting cancer therapy has not been feasible due to generalized effects on all glycoproteins. Here, we perform sensitivity screening 94 lung cell lines using NGI-1, small-molecule inhibitor oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate selective loss tumor viability. This...
<div>Abstract<p>Asparagine (N)-linked glycosylation is a posttranslational modification essential for the function of complex transmembrane proteins. However, targeting cancer therapy has not been feasible due to generalized effects on all glycoproteins. Here, we perform sensitivity screening 94 lung cell lines using NGI-1, small-molecule inhibitor oligosaccharyltransferase (OST) that partially disrupts N-linked glycosylation, and demonstrate selective loss tumor viability. This...
<p>Deep sequencing of lung cancer cell lines to determine the proportion EGFR mutations.</p>
<p>Results of the Lung Cancer Cell Line Screen for OST Inhibitor Sensitivity</p>
<p>Legends for supplementary figures 1 and 2</p>
<p>Characterization of the Osimertinib resistant H1975 cells.</p>
<p>Apoptosis susceptibility of KRAS mutant A549 cell line following treatment with NGI-1, Erlotinib or both.</p>
<p>Supplementary figures legends</p>
<p>Dose response of NGI-1, temozolomide and etoposide in glioma cell lines</p>
<p>Time course resolution of γH2AX foci formation in D54 cells</p>
<p>Supplementary figures legends</p>
<p>Time course resolution of γH2AX foci formation in D54 cells</p>
<p>Dose response of NGI-1, temozolomide and etoposide in glioma cell lines</p>
<div>AbstractPurpose:<p>Parallel signaling reduces the effects of receptor tyrosine kinase (RTK)–targeted therapies in glioma. We hypothesized that inhibition protein N-linked glycosylation, an endoplasmic reticulum co- and posttranslational modification crucial for RTK maturation activation, could provide a new therapeutic approach glioma radiosensitization.</p><p><b>Experimental Design:</b> investigated small-molecule inhibitor oligosaccharyltransferase...
<div>AbstractPurpose:<p>Parallel signaling reduces the effects of receptor tyrosine kinase (RTK)–targeted therapies in glioma. We hypothesized that inhibition protein N-linked glycosylation, an endoplasmic reticulum co- and posttranslational modification crucial for RTK maturation activation, could provide a new therapeutic approach glioma radiosensitization.</p><p><b>Experimental Design:</b> investigated small-molecule inhibitor oligosaccharyltransferase...