A5068589576- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Nitric Oxide and Endothelin Effects
- Cardiovascular Disease and Adiposity
- Sperm and Testicular Function
- Mitochondrial Function and Pathology
- Ferroptosis and cancer prognosis
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Diet and metabolism studies
- Advanced Glycation End Products research
- Reproductive Biology and Fertility
- Genomics, phytochemicals, and oxidative stress
- MicroRNA in disease regulation
- Selenium in Biological Systems
- Thermoregulation and physiological responses
- Eicosanoids and Hypertension Pharmacology
- Diet, Metabolism, and Disease
- Fatty Acid Research and Health
- Birth, Development, and Health
- Biochemical effects in animals
- Redox biology and oxidative stress
- RNA modifications and cancer
- ATP Synthase and ATPases Research
- Thermal Regulation in Medicine
University of Belgrade
2016-2025
Institute for Biological Research “Siniša Stanković”
2012-2019
Centar za Promociju Nauke
2015
The main pathological hallmark of diabetes is the loss functional β-cells. Among several types β-cell death in diabetes, involvement ferroptosis remains elusive. Therefore, we investigated potential diabetes-mimicking factors: high glucose (HG), proinflammatory cytokines, hydrogen peroxide (H2O2), or diabetogenic agent streptozotocin (STZ) to induce β-cells vitro. Furthermore, tested contribution injury pancreatic islets an STZ-induced vivo diabetic model. All vitro treatments increased...
Abstract Recently, we characterized the ferroptotic phenotype in liver of diabetic mice and revealed nuclear factor (erythroid‐derived‐2)‐related 2 (Nrf2) inactivation as an integral part hepatic injury. Here, aim to investigate whether sulforaphane, Nrf2 activator antioxidant, prevents diabetes‐induced ferroptosis mechanisms involved. Male C57BL/6 were divided into four groups: control (vehicle‐treated), (streptozotocin‐induced; 40 mg/kg, from Days 1 5), sulforaphane‐treated (2.5 mg/kg 42)...
Key points White to brown adipose tissue conversion and thermogenesis can be ignited by different conditions or agents its sustainability over the long term is still unclear. Browning of rat retroperitoneal white (rpWAT) during cold acclimation involves two temporally apparent components: (1) a predominant non‐selective browning most adipocytes an initial sharp but transient induction uncoupling protein 1, peroxisome proliferator‐activated receptor (PPAR) coactivator‐1α, PPARγ PPARα...
Background . Semen analysis is the cornerstone in evaluation of male (in)fertility. However, there are men with normal semen tests but impaired fertilizing ability, as well fertile poor sperm characteristics. Thus, rising interest to find novel parameters that will help predict and define functional capacity spermatozoa. Methods We examined whether a correlation between (count, progressive motility, morphology) protein expression/activity antioxidative defense enzymes seminal plasma from 10...
Cell death plays an important role in diabetes-induced liver dysfunction. Ferroptosis is a newly defined regulated cell caused by iron-dependent lipid peroxidation. Our previous studies have shown that high glucose and streptozotocin (STZ) cause β-cell through ferroptosis ferrostatin-1 (Fer-1), inhibitor of ferroptosis, improves viability, islet morphology, function. This study was aimed to examine vivo the involvement diabetes-related pathological changes liver. For this purpose, male...
Brown adipose tissue (BAT) has the unique ability of generating heat due to expression mitochondrial uncoupling protein 1 (UCP1). A recent discovery regarding functional BAT in adult humans increased interest molecular pathways development and functionality. An important role for estrogen white was shown, but possible human fetal (fBAT) is unclear.The objective this study determine whether fBAT expresses receptor α (ERα) ERβ. In addition, we examined their localization as well correlation...
Metabolic syndrome arises from abnormal adipose function accompanied by insulin resistance. As early factors reflecting/impacting lipid storage dysfunction of tissues, we sought to determine adipokine levels in subcutaneous and visceral tissues (SAT VAT).Gene protein expression leptin, adiponectin, resistin were analysed SAT VAT normal-weight overweight/obese women, subclassified according resistance index, triglyceride, total, low-density lipoprotein (LDL) high-density (HDL) cholesterol...
Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes redox milieu affect the functional status of mitochondria, that is, functionality. Compared with control, incubation Tyrode's medium for 3 h, under noncapacitating conditions, decreased motility, amount nitric oxide ((•)NO), number MitoTracker(®) Green FM (MT-G) positive expression complexes I IV mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic...
Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane propofol cardioprotection during aortic valve replacement surgery due to stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding anesthetic regime: propofol. Hemodynamic parameters, biomarkers cardiac injury brain natriuretic peptide (BNP) measured preoperatively postoperatively. In tissue samples, taken from interventricular septum, key...
The expression profiles of adiponectin, resistin, 5′-AMP-activated protein kinase α (AMPK ), hypoxia-inducible factor-1 (HIF-1 and key enzymes glucose fatty acid metabolism oxidative phosphorylation in rat retroperitoneal white adipose tissue (RpWAT) during 45-day cold acclimation were examined. After transient suppression on day 1, adiponectin level increased following sustained exposure. In parallel, the HIF-1 was strongly induced AMPK suppressed, while afterwards reverse seen. What is...
Mitochondria are key organelles maintaining cellular bioenergetics and integrity, their regulation of [Ca2+]i homeostasis has been investigated in many cell types. We the short-term Ca-SANDOZ® treatment on brown adipocyte mitochondria, using imaging molecular biology techniques. Two-month-old male Wistar rats were divided into two groups: drinking or tap water (control) for three days. Alizarin Red S staining showed increased Ca2+ level adipocytes treated rats, potassium pyroantimonate...
The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy also, possible mechanisms underlying ECs death. In order induce adult male rats Wistar strain were treated with high dose (4 U/kg, intraperitonealy) for one or three days. Examinations at ultrastructural level showed apoptotic changes ECs, allowing us...